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The pivotal role of micro-environmental cells in a human blood –brain barrier in vitro model of cerebral ischemia: functional and transcriptomic analysis
ConclusionsThe co-cultivation with micro-environmental cells is necessary to achieve a functional breakdown of the BBB in the cerebral ischemia model within an in vivo relevant time window. Comprehensive studies by qPCR revealed that distinct expression clusters of barrier markers exist and that these are regulated by different treatments (even by growth medium change) indicating that controls for single cell culture manipulation steps are crucial to understand the observed effects properly.
Source: Fluids and Barriers of the CNS - March 4, 2020 Category: Neuroscience Source Type: research
