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Study on the Role of Mitophagy Receptor PHB2 in Doubly Uniparental Inheritance of Hyriopsis cumingii
Mar Biotechnol (NY). 2023 Aug 18. doi: 10.1007/s10126-023-10240-5. Online ahead of print.ABSTRACTIn bivalves, the heterogeneity of mitochondrial DNA and its unique mode of transmission have been the focus of attention, which is called doubly uniparental inheritance (DUI). Prohibitin-2 (phb2) is a mitochondrial inner membrane protein that is a key mitophagy receptor for parental mitochondrial removal. Hyriopsis cumingii is a freshwater bivalve in China, the full-length cDNA of H. cumingii phb2 (named Hcphb2) is 2917 bp and encodes a total of 300 amino acids, a highly conserved sequence. Hcphb2 was highly expressed in the ov...
Source: Marine Biotechnology - August 18, 2023 Category: Biotechnology Authors: Yayu Wang Xiaoyue Zhu Yang Gu Zongyu Liu Yingrui Mao Xiaojun Liu Zhiyi Bai Guiling Wang Jiale Li Source Type: research

Molecular Characterization of U6 Promoters from Orange-Spotted Grouper (Epinephelus coioides) and Its Application in DNA Vector-Based RNAi Technology
Mar Biotechnol (NY). 2023 May 8. doi: 10.1007/s10126-023-10212-9. Online ahead of print.ABSTRACTThe U6 promoter, a typical RNA polymerase III promoter, is widely used to transcribe small RNAs in vector-based siRNA systems. The RNAi efficiency is mainly dependent on the transcriptional activity of the U6 promoter. However, studies have found that U6 promoters isolated from some fishes do not work well in distantly related species. To isolate a U6 promoter with high transcriptional efficiency from fish, in this study, we cloned five U6 promoters in orange-spotted grouper, of which only the grouper U6-1 (GU6-1) promoter conta...
Source: Marine Biotechnology - May 8, 2023 Category: Biotechnology Authors: Fengying Yan Xinxun Xiao Chen Long Lin Tang Chongwei Wang Mingqing Zhang Jin Zhang Haoran Lin Hai Huang Yong Zhang Shuisheng Li Source Type: research

Comparative Transcriptome Reveals the Potential Modulation Mechanisms of Spfoxl-2 Affecting Ovarian Development of Scylla paramamosain
Mar Biotechnol (NY). 2022 Feb 2. doi: 10.1007/s10126-022-10091-6. Online ahead of print.ABSTRACTPreviously, we reported the identification, tissue distribution and confirmed the roles of Spfoxl-2 in regulating vitellogenin (vtg) expression in Scylla paramamosain. Here, we primally analyzed its potential target genes in the ovary with RNAi and RNA-Seq technology. By comparing the transcriptome data of two groups (ovaries that injected with EGFP and Foxl-2 siRNA, respectively), we found 645 DEGs (differentially expressed genes), including several conserved crucial genes involved in ovarian development, such as vtg, vitelloge...
Source: Marine Biotechnology - February 2, 2022 Category: Biotechnology Authors: Haifu Wan Jinying Zhong Ziping Zhang Pengfei Zou Yilei Wang Source Type: research

siRNA-Mediated MrIAG Silencing Induces Sex Reversal in Macrobrachium rosenbergii.
Abstract The insulin-like androgenic gland (IAG) gene is well known in male crustacean, and it is a key regulator in male sexual differentiation and maintaining the male sexual characteristic. The neo-female can be produced by silencing the MrIAG (Macrobrachium rosenbergii Insulin-like Androgenic Gland) in male Macrobrachium rosenbergii. This is the first time to use siRNA approach to silenced MrIAG in male M. rosenbergii. In the current study, the optimal injection dosage to achieve sex reversal is 0.5 μg/g body weight. After MrIAG silencing, the expression level of Dmrt11e, Dmrt99b, MRPINK, Mrr, Sxl1, and Sxl2...
Source: Marine Biotechnology - April 26, 2020 Category: Biotechnology Authors: Tan K, Zhou M, Jiang H, Jiang D, Li Y, Wang W Tags: Mar Biotechnol (NY) Source Type: research

Chitosan-Mediated shRNA Knockdown of Cytosolic Alanine Aminotransferase Improves Hepatic Carbohydrate Metabolism.
In this study, we examined the effect of silencing cytosolic ALT (cALT) expression on the hepatic metabolism in Sparus aurata. A number of siRNA and shRNA designed to down-regulate cALT expression were validated in HEK-293 cells transfected with plasmids expressing S. aurata cALT or mitochondrial ALT (mALT) isoforms: ALT silencing significantly decreased the expression levels of S. aurata mRNA cALT1 to 62 % (siRNA) and 48 % (shRNA) of the values observed in control cells. The effect of cALT silencing was analysed in the liver of S. aurata 72 h after intraperitoneal injection of chitosan-tripolyphosphate (TPP) nanopartic...
Source: Marine Biotechnology - October 16, 2015 Category: Biotechnology Authors: González JD, Silva-Marrero JI, Metón I, Caballero-Solares A, Viegas I, Fernández F, Miñarro M, Fàbregas A, Ticó JR, Jones JG, Baanante IV Tags: Mar Biotechnol (NY) Source Type: research

Microarray Analysis of Immunity Against WSSV in Response to Injection of Non-specific Long dsRNA in Kuruma Shrimp, Marsupenaeus japonicus.
Abstract Injection of shrimp with non-specific double-stranded RNA (dsRNA) of diverse lengths, sequences, and base compositions is known to induce non-specific immunity and protect against lethal disease, although the mechanisms are unclear. Previous shrimp studies examined the effects of non-specific RNA on particular pathways, while their global effects have not been examined. To understand the global effects of non-specific RNA in shrimp, we injected kuruma shrimp (Marsupenaeus japonicus) with a dsRNA and a small interfering RNA (siRNA) that is not specific to any gene in the shrimp genome and then examined glo...
Source: Marine Biotechnology - May 8, 2015 Category: Biotechnology Authors: Maralit BA, Komatsu M, Hipolito SG, Hirono I, Kondo H Tags: Mar Biotechnol (NY) Source Type: research