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Alternative splicing of a carboxyl/choline esterase gene enhances the fenpropathrin tolerance of Tetranychus cinnabarinus
This article is protected by copyright. All rights reserved
Source: Insect Science - December 22, 2022 Category: Biology Authors: Peng Wei, Xinying Zeng, Haonan Han, Yiqing Yang, Youjun Zhang, Lin He Tags: ORIGINAL ARTICLE Source Type: research

The clock gene, period, influences migratory flight and reproduction of the oriental armyworm, Mythimna separata
This article is protected by copyright. All rights reserved
Source: Insect Science - October 29, 2022 Category: Biology Authors: Jiayue Ji, Yueqiu Liu, Lei Zhang, Yunxia Cheng, David Stanley, Xingfu Jiang Tags: ORIGINAL ARTICLE Source Type: research

Mblk ‐1 regulates sugar responsiveness in honey bee (Apis mellifera) foragers
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Source: Insect Science - September 28, 2021 Category: Biology Authors: Fang Liu, Lixian Wu, Yuan Zhang, Qiang Li, Liangbin Li, Zachary Y. Huang, Hongxia Zhao Tags: ORIGINAL ARTICLE Source Type: research

BmSd gene regulates the silkworm wing size by affecting the Hippo pathway
This article is protected by copyright. All rights reserved
Source: Insect Science - June 20, 2019 Category: Biology Authors: Jin Yin, Jing Zhang, Tao Li, Xia Sun, Sheng Qin, Cheng ‐Xiang Hou, Guo‐Zheng Zhang, Mu‐Wang Li Tags: ORIGINAL ARTICLE Source Type: research

Systemic RNAi in western corn rootworm, Diabrotica virgifera virgifera, does not involve transitive pathways
This study visualized the spread of the RNAi‐mediated knockdown of Dv v‐ATPase C mRNA throughout the WCR gut and other tissues using high‐sensitivity branched DNA in situ hybridization. Furthermore, we did not detect either secondary siRNA production or transitive RNAi in WCR through siRNA sequence profile analysis. Nucleotide mismatched sequences introduced into either the sense or antisense strand of v‐ATPase C dsRNAs were maintained in siRNAs derived from WCR fed with the mismatched dsRNAs in a strand specific manner. The distribution of all siRNAs was restricted to within the original target sequence regions, w...
Source: Insect Science - November 16, 2016 Category: Biology Authors: Huarong Li, Andrew J. Bowling, Premchand Gandra, Murugesan Rangasamy, Heather E. Pence, Robert E. McEwan, Chitvan Khajuria, Blair D. Siegfried, Kenneth E. Narva Tags: ORIGINAL ARTICLE Source Type: research

Systemic RNAi in western corn rootworm, Diabrotica virgifera virgifera LeConte, does not involve transitive pathways
This article is protected by copyright. All rights reserved
Source: Insect Science - July 31, 2016 Category: Biology Authors: Huarong Li, Andrew J. Bowling, Premchand Gandra, Murugesan Rangasamy, Heather E. Pence, Robert McEwan, Chitvan Khajuria, Blair Siegfried, Kenneth E. Narva Tags: ORIGINAL ARTICLE Source Type: research

Silencing of odorant binding protein gene AlinOBP4 by RNAi induces declining electrophysiological responses of Adelphocoris lineolatus to six semiochemicals
Abstract Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. Here, we injected AlinOBP4‐siRNA into the conjunctivum between prothorax and mesothorax of the lucerne plant bug, Adelphocoris lineolatus and evaluated the silencing of AlinOBP4 by reverse transcription polymerase chain reaction (RT‐PCR) analysis, quantitative real‐time PCR (qPCR) test and electroantennogram (EAG) assay. The combination of RT‐PCR and qPCR analyses revealed that the levels of messenger RNA transc...
Source: Insect Science - July 25, 2016 Category: Biology Authors: Xue ‐Ying Zhang, Xiao‐Qiang Zhu, Shao‐Hua Gu, Yan‐Le Zhou, Song‐Ying Wang, Yong‐Jun Zhang, Yu‐Yuan Guo Tags: ORIGINAL ARTICLE Source Type: research

Silencing of odorant binding protein gene AlinOBP4 by RNAi induce electrophysiological responses declining of Adelphocoris lineolatus to six semiochemicals
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Source: Insect Science - June 5, 2016 Category: Biology Authors: Xue‐Ying Zhang, Xiao‐Qiang Zhu, Shao‐Hua Gu, Yan‐Le Zhou, Song‐Ying Wang, Yong‐Jun Zhang, Yu‐Yuan Guo Tags: ORIGINAL ARTICLE Source Type: research

Influence of various stressors on the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis
Abstract RNA interference (RNAi)‐based technology has emerged as a potential tool for controlling insect pests, however, previous studies found that the efficiency of RNAi in Bactrocera dorsalis was variable. In nature, insects often meet various challenges, such as pathogen infections, extreme temperatures, lack of nutrition and heavy metals. To better understand the association of the stressors with efficiency of RNAi, in the current study we tested the expression of three core genes, dicer2 (Bddcr2), r2d2 (Bdr2d2) and argonaute2 (Bdago2), of the small interfering RNA (siRNA) pathway of B. dorsalis upon various stresso...
Source: Insect Science - May 24, 2016 Category: Biology Authors: Yi‐Fei Xie, Jin‐Zhi Niu, Xuan‐Zhao Jiang, Wen‐Jia Yang, Guang‐Mao Shen, Dong Wei, Guy Smagghe, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research

Influence of various stressors to the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis (Hendel)
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Source: Insect Science - January 6, 2016 Category: Biology Authors: Yi‐Fei Xie, Jin‐Zhi Niu, Xuan‐Zhao Jiang, Wen‐Jia Yang, Guang‐Mao Shen, Dong Wei, Guy Smagghe, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research

Advance of RNA interference technique in Hemipteran insects
ABSTRACTRNA interference (RNAi) suppressed the expression of the target genes by post transcriptional regulation and the double‐stranded RNA (dsRNA) mediated gene silencing has been a conserved mechanism in many eukaryotes, which prompted RNAi to become a valuable tool for unveiling the gene function in many model insects. Recent research attested that RNAi technique can be also effective in downregulation target genes in Hemipteran insects. In this review, we collected the researches of utilizing RNAi technique in gene functional analysis in Hemipteran insects, highlighted the methods of dsRNA/siRNA uptake by insects an...
Source: Insect Science - July 24, 2012 Category: Biology Authors: Jie Li, Xiaoping Wang, Manqun Wang, Weihua Ma, Hongxia Hua Tags: REVIEW Source Type: research