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UV ‐induced DNA Damage in Skin is Reduced by CaSR Inhibition
In this study we investigated whether the CaSR is involved more directly in protection from UV damage in studies of human keratinocytes in primary culture and in mouse skin studiedin vivo. siRNA-directed reductions in CaSR protein levels in human keratinocytes significantly reduced UV-induced direct cyclobutane pyrimidine dimers (CPD) by around 80% and oxidative DNA damage (8-OHdG) by around 65% compared to control transfected cells. Similarly, in untransfected cells, the CaSR negative modulator, NPS-2143 (500 nM), reduced UV-induced CPD and 8-OHdG by around 70%. NPS-2143 also enhanced DNA repair and reduced reactive oxyge...
Source: Photochemistry and Photobiology - March 15, 2022 Category: Science Authors: Chen Yang, Mark Stephen Rybchyn, Warusavithana Gunawardena Manori De Silva, Jim Matthews, Andrew J.A. Holland, Arthur David Conigrave, Rebecca Sara Mason Tags: RESEARCH ARTICLE Source Type: research

Melanopsin Signaling Pathway in HEK293 Cell Line with Stable Expression of Human Melanopsin: Possible Participation of Phospholipase C beta 4 and Diacylglycerol
AbstractMelanopsin, a member of the G-protein coupled receptors family, is involved in non-image-forming functions including circadian rhythm, sleep regulation, pupil response. In spite of significant research efforts, the signaling cascade involving melanopsin photoactivation remains poorly characterized. Here, we analyzed the effects of photoactivation of melanopsin on phospholipase C (PLC) and diacylglycerol. As anin vitro model, HEK293 cells with stable expression of human melanopsin were used. Although both the PLCβ1 and PLCβ4 subtypes were activated by the cell exposure to blue light, only PLCβ4 appeared to play a...
Source: Photochemistry and Photobiology - May 12, 2021 Category: Science Authors: Olga I. Krzysztynska ‐Kuleta, Magdalena M. Olchawa, Tadeusz J. Sarna Tags: RESEARCH ARTICLE Source Type: research