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Differential effects of follistatin on porcine oocyte competence and cumulus cell gene expression in vitro
This study investigated the paracrine and autocrine activity of follistatin (FS), which is secreted by oocytes and cumulus cells as part of porcine embryogenesis. Immunohistochemical (IHC) localization of follistatin was conducted on 100 randomly selected early‐ and late‐cleaving two‐cell embryos. Dissociated cumulus cells were treated with various doses of follistatin for determination of the follistatin gene (FST) mRNA expression levels by quantitative real‐time PCR analysis. Microinjection of siRNA induced a downregulation of FST mRNA during embryonic development, thereby decreasing the proportion embryos develo...
Source: Reproduction in Domestic Animals - November 14, 2017 Category: Reproduction Medicine Authors: Z Guo, MS Islam, D Liu, G Liu, L Lv, Y Yang, B Fu, L Wang, Z Liu, H He, H Wu Tags: ORIGINAL ARTICLE Source Type: research

miR ‐182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro
In conclusion, miR‐182 targeted HOXA10 selectively in EECs in vitro, and HOXA10 played an important role in maintaining the function of EECs in dairy goats.
Source: Reproduction in Domestic Animals - July 30, 2017 Category: Reproduction Medicine Authors: L Zhang, X ‐R Liu, J‐Z Liu, Y‐X Song, Z‐Q Zhou, B‐Y Cao Tags: ORIGINAL ARTICLE Source Type: research