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GATA4 knockdown in MA-10 Leydig cells identifies multiple target genes in the steroidogenic pathway
GATA4 is an essential transcription factor required for the initiation of genital ridge formation, for normal testicular and ovarian differentiation at the time of sex determination, and for male and female fertility in adulthood. In spite of its crucial roles, the genes and/or gene networks that are ultimately regulated by GATA4 in gonadal tissues remain to be fully understood. This is particularly true for the steroidogenic lineages such as Leydig cells of the testis where many in vitro (promoter) studies have provided good circumstantial evidence that GATA4 is a key regulator of Leydig cell gene expression and steroidog...
Source: Reproduction - February 6, 2015 Category: Reproduction Medicine Authors: Bergeron, F., Nadeau, G., S Viger, R. Tags: Research Source Type: research

Regulation of ovulatory genes in bovine granulosa cells: lessons from siRNA silencing of PTGS2
In this study, we compared the induction of these ovulatory genes in bovine granulosa cells (GCs) in vivo (after LH surge) and in vitro (forskolin (FRS) treatment). These genes were markedly stimulated in GCs isolated from cows 21 h after LH-surge. In isolated GCs, FRS induced a distinct temporal profile for each gene. Generally, there was a good agreement between the in vivo and in vitro inductions of these genes except for PTX3. Lack of PTX3 induction in isolated GCs culture suggests that other follicular compartments may mediate its induction by LH. Next, to study the role of PTGS2 and prostaglandins (PGs) in the cascad...
Source: Reproduction - November 28, 2014 Category: Reproduction Medicine Authors: Shrestha, K., Lukasik, K., Baufeld, A., Vanselow, J., Moallem, U., Meidan, R. Tags: Research Source Type: research

REGULATION OF OVULATORY GENES IN BOVINE GRANULOSA CELLS: LESSONS FROM siRNA SILENCING OF PTGS2.
Abstract Prostaglandin endoperoxide synthase 2 (PTGS2), tumor necrosis factor-alpha-induced protein-6 (TNFAIP6), pentraxin-3 (PTX3), epidermal growth factor like factors: amphiregulin (AREG) and epiregulin (EREG) are essential for successful ovulation. We compared here the induction of these ovulatory genes in bovine granulosa cells (GCs) in vivo (after LH surge) and in vitro (forskolin treatment). These genes were markedly stimulated in GCs isolated from cows 21hr after LH- surge. In isolated GCs, forskolin induced a distinct temporal profile for each gene. Generally, there was a good agreement between the in viv...
Source: Reproduction - October 16, 2014 Category: Reproduction Medicine Authors: Shrestha K, Lukasik K, Baufeld A, Vanselow J, Moallem U, Meidan R Tags: Reproduction Source Type: research

The Nuclear Receptor NR2F2 Activates Star Expression and Steroidogenesis in Mouse MA-10 and MLTC-1 Leydig Cells.
In this study, we found that NR2F2 is expressed in the nucleus of some peritubular myoid cells and in interstitial cells, mainly in steroidogenically active adult Leydig cells. In MA-10 and MLTC-1 Leydig cells, siRNA-mediated NR2F2 knockdown reduces basal steroid production without affecting hormone responsiveness. Consistent with this, we found that STAR mRNA and protein levels were reduced in NR2F2-depleted MA-10 and MLTC-1 cells. Transient transfections of Leydig cells revealed that a -986 bp mouse Star promoter construct was activated 3 fold by NR2F2. Using 5' progressive deletion constructs, we mapped the NR2F2-respon...
Source: Biology of Reproduction - June 4, 2014 Category: Reproduction Medicine Authors: Mendoza-Villarroel RE, Robert NM, Martin LJ, Brousseau C, Tremblay JJ Tags: Biol Reprod Source Type: research