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Preliminary evaluation of a small interfering RNA molecular probe targeting murine double minute 2 in breast cancer
Conclusion The [99mTc]HYNIC-siRNA 1489 was taken up by the tumor, which had a high level of MDM2. The probe exhibited a sufficient retention time in the tumor. This probe may be an effective strategy for evaluating MDM2 expression and achieving early diagnosis in breast cancer.
Source: Nuclear Medicine Communications - July 21, 2022 Category: Nuclear Medicine Tags: Original Articles Source Type: research

Synthesis of a stabilized 177Lu–siRNA complex and evaluation of its stability and RNAi activity
In this study, the effects of PS and 2′-O-Me modifications at the backbone of siRNA on serum stability and RNA interference activity were investigated. Materials and methods: Fully PS and 2′-O-Me-modified type 1 insulin-like growth factor receptor (IGF-1R) siRNA was radiolabeled with lutetium-177 (177Lu) through p-SCN-Bn-DTPA as a chelator. After purification with Vivaspin and PD-10 columns, the radiolabs were examined for stability in serum by instant thin-layer chromatography and polyacrylamide gel electrophoresis. The level of IGF-1R in response to the modified and labeled IGF-1R siRNA was examined using RT-PCR and ...
Source: Nuclear Medicine Communications - April 28, 2015 Category: Nuclear Medicine Tags: Original Articles Source Type: research