• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Synthesis of a stabilized 177Lu–siRNA complex and evaluation of its stability and RNAi activity
In this study, the effects of PS and 2′-O-Me modifications at the backbone of siRNA on serum stability and RNA interference activity were investigated. Materials and methods: Fully PS and 2′-O-Me-modified type 1 insulin-like growth factor receptor (IGF-1R) siRNA was radiolabeled with lutetium-177 (177Lu) through p-SCN-Bn-DTPA as a chelator. After purification with Vivaspin and PD-10 columns, the radiolabs were examined for stability in serum by instant thin-layer chromatography and polyacrylamide gel electrophoresis. The level of IGF-1R in response to the modified and labeled IGF-1R siRNA was examined using RT-PCR and ...
Source: Nuclear Medicine Communications - April 28, 2015 Category: Nuclear Medicine Tags: Original Articles Source Type: research