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17 β-estradiol inhibits H2O2-induced senescence in HUVEC cells through upregulating SIRT3 expression and promoting autophagy.
In this study, we examined the roles of SIRT3 in 17β-E2-induced autophagy and 17β-E2-mediated inhibition of hydrogen peroxide (H2O2)-induced senescence in Human umbilical vein endothelial cells (HUVEC). Cellular senescence was measured by immunoblot analysis with antibodies against phosphorylated Rb and senescence-associated β-galactosidase staining. Immunoblot analysis with antibodies against LC3 and p62 was performed to determine autophagy flux. Our findings show that 17β-E2 activates SIRT3 promoter and upregulates SIRT3 gene expression in HUVEC cells. siRNA-mediated silencing of SIRT3 gene expression inhibits 17β-E...
Source: Biogerontology - March 13, 2020 Category: Geriatrics Authors: Xiang X, Huang J, Song S, Wang Y, Zeng Y, Wu S, Ruan Y Tags: Biogerontology Source Type: research