• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Rac-specific nucleotide exchange factor Asef mediates lung endothelial barrier protection by hepatocyte growth factor
This study tested the role of Asef in the HGF-induced activation of endothelial cell (EC) cytoskeletal dynamics and vascular endothelial barrier protective response in vitro and in two-hit animal model of ALI. HGF induced membrane translocation of Asef and stimulated its Rac-specific nucleotide exchange activity. Expression of constitutively activated Asef mutant mimicked effects of HGF on enhancement of peripheral actin cytoskeleton. By contrast, siRNA-induced Asef knockdown or expression of dominant negative Asef attenuated HGF-induced Rac activation which was evaluated using Rac-GTP pulldown assay and FRET analysis with...
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Birukova, A., Tian, Y., Meng, F. Tags: 3.3 Mechanisms of Lung Injury and Repair Source Type: research

Microtubules mediate anti-inflammatory and lung vascular barrier protective effects of atrial natriuretic peptide
Independently on control of circulating fluid, atrial natriuretic peptide (ANP) exhibits anti-inflammatory effects in the lung. We tested a role of microtubule (MT) cytoskeleton in the ANP-induced protection against endothelial cell (EC) barrier dysfunction and acute lung injury induced by Staphylococcus aureus-derived peptidoglican-G (PepG). ANP attenuated PepG-induced Rho signaling via suppression of PepG-induced MT disassembly and inhibition of MT-associated Rho-specific guanine nucleotide exchange factor GEF-H1. ANP-induced EC barrier preservation and MT stabilization was due to phosphorylation and inactivation of MT-a...
Source: European Respiratory Journal - December 23, 2014 Category: Respiratory Medicine Authors: Birukova, A., Shah, A., Tian, Y., Meng, F. Tags: 3.3 Mechanisms of Lung Injury and Repair Source Type: research