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UBXN1 interacts with the S1 protein of transmissible gastroenteritis coronavirus and plays a role in viral replication
AbstractTransmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs and is associated with high morbidity and mortality in sucking piglets. S1 is one of two protein domains in the spike (S) glycoprotein and is responsible for enteric tropism, sialic acid recognition, and host receptor binding. Although there has been extensive research on the S1 protein of TGEV, little is known about the intracellular role of TGEV-S1. In the present study, we used yeast two-hybrid screening of a cDNA library from porcine intestinal cells to identify proteins that interact with TGEV-S1. A...
Source: Veterinary Research - April 26, 2019 Category: Veterinary Research Source Type: research

EIF4A2 interacts with the membrane protein of transmissible gastroenteritis coronavirus and plays a role in virus replication
Publication date: Available online 17 December 2018Source: Research in Veterinary ScienceAuthor(s): Zhenhui Song, Yang Yang, Li Wang, Kai Wang, Ling Ran, Yilu Xie, LeiShi Huang, Zhou Yang, Peng Yuan, Qiuhan YuAbstractTransmissible gastroenteritis coronavirus (TGEV) is enteropathogenic coronavirus that causes diarrhea in pigs, and is associated with high morbidity and mortality in sucking piglets. The TGEV membrane (M) protein is a decisive protein for the proliferation of viral proteins, and is associated with virus assembly and budding. To identify the cellular proteins that interact with the TGEV M protein, yeast two-hyb...
Source: Research in Veterinary Science - December 18, 2018 Category: Veterinary Research Source Type: research

Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II
AbstractCaliciviruses in the genusSapovirus are a significant cause of viral gastroenteritis in humans and animals. However, the mechanism of their entry into cells is not well characterized. Here, we determined the entry mechanism of porcine sapovirus (PSaV) strain Cowden into permissive LLC-PK cells. The inhibition of clathrin-mediated endocytosis using chlorpromazine, siRNAs, and a dominant negative (DN) mutant blocked entry and infection of PSaV Cowden strain, confirming a role for clathrin-mediated internalization. Entry and infection were also inhibited by the cholesterol-sequestering drug methyl- β-cyclodextrin and...
Source: Veterinary Research - September 17, 2018 Category: Veterinary Research Source Type: research