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siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic sequences
Virology. 2022 Dec 31;579:38-45. doi: 10.1016/j.virol.2022.12.013. Online ahead of print.ABSTRACTDue to the recurrent pandemic outbreaks that occurred during the last century, Influenza A viruses are considered a serious potential danger to human health. Among the innate immune pathways in eukaryotes, RNA interference plays a significant role in the interaction between viruses and host cells. RNA interference is addressed by small dsRNA molecules produced by the host itself (miRNAs, i.e. "micro-RNAs") but can be triggered also by the administration of exogenous short RNAs (siRNAs, "short interfering RNAs"). In this work, a...
Source: Virology - January 4, 2023 Category: Virology Authors: Riccardo Villa Sabrina Renzi Silvia Dotti Franco Lucchini Source Type: research

Viruses, Vol. 14, Pages 359: Encephalomyocarditis Virus 2A Protein Inhibited Apoptosis by Interaction with Annexin A2 through JNK/c-Jun Pathway
In this study, the 2A protein was expressed in Escherichia coli in order to find interacting cell proteins. A pull down assay, coupled with mass spectrometry, revealed that the 2A protein possibly interacted with annexin A2. Co-immunoprecipitation assays and confocal imaging analysis further demonstrated that the 2A protein interacted with annexin A2 in cells. In reducing the expression of annexin A2 by siRNA, the ability of the 2A protein to inhibit apoptosis was weakened and the proliferation of EMCV was slowed down. These results suggest that annexin A2 is closely related to the inhibition of apoptosis by 2A. Furthermor...
Source: Viruses - February 9, 2022 Category: Virology Authors: Ruochan Han Lin Liang Tong Qin Sa Xiao Ruiying Liang Tags: Article Source Type: research

Development of transgenic okra (Abelmoschus esculentus L. Moench) lines having RNA mediated resistance to Yellow vein mosaic virus (Geminiviridae)
J Virol Methods. 2022 Jan 6;301:114457. doi: 10.1016/j.jviromet.2022.114457. Online ahead of print.ABSTRACTBegomovirus Yellow vein mosaic virus causes severe yield losses in okra and even the resistant lines developed through conventional breeding show susceptibility at various levels. This paper describes the development of YVMV resistant lines through RNAi strategy. A universal ihpRNA construct harbouring βC1 ORF from the β-satellite of the begomovirus was designed using pRNAi-LIC plasmid. Complementarity checks in sequence databases had shown no off-target effects by the target region and the success of siRNA in inter...
Source: Journal of Virological Methods - January 9, 2022 Category: Virology Authors: Kelkar Vipul Ganesh Deepu Mathew Rehna Augustine Soni K B Swapna Alex Shylaja M R Anita Cherian K Source Type: research