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Interleukin 10 suppresses lysosome-mediated killing of Brucella abortus in cultured macrophages Immunology
In this study, we investigated the regulatory role of IL10 in the immune response to B. abortus infection. B. abortus–infected macrophages were treated with either IL10 siRNA or recombinant IL10 (rIL10), and the expression of phagolysosome- or inflammation-related genes was evaluated by qRT-PCR and Western blotting. Phagolysosome fusion was monitored by fluorescence microscopy. We found that the synthesis of several membrane-trafficking regulators and lysosomal enzymes was suppressed by IL10 during infection, resulting in a significant increase in the recruitment of hydrolytic enzymes by Brucella-containing phagosomes (B...
Source: Journal of Biological Chemistry - March 2, 2018 Category: Chemistry Authors: Huynh Tan Hop, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim Tags: Microbiology Source Type: research