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Source: Redox Biology
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Total 62 results found since Jan 2013.
Specific Delivery of Delta-5-Desaturase siRNA via RNA Nanoparticles Supplemented with Dihomo-γ-Linolenic Acid for Colon Cancer Suppression
In this study, we employed RNA nanotechnology for specific delivery of D5D-siRNA to xenograft colon tumors using 3WJ RNA nanoparticles. When a targeting module, i.e., the EpCAM aptamer, was incorporated, the 3WJ pRNA nanoparticles were able specifically deliver D5D siRNA to human colon cancer HCA-7 cells both in vitro and in vivo, resulting in significant downregulation of D5D expression. Co-treatment with DGLA in combination with 3WJ-EpCAM-siRNA induced a higher DGLA/AA ratio and enhanced formation of 8-HOA at a threshold level, and in HCA-7 tumor-bearing mice, induced significant tumor suppression. We further confirmed t...
Source: Redox Biology - December 19, 2018 Category: Biology Source Type: research
The Mitochondria-targeted Antioxidant MitoQ Ameliorated Tubular Injury Mediated by Mitophagy in Diabetic Kidney Disease via Nrf2/PINK1
Publication date: Available online 21 December 2016 Source:Redox Biology Author(s): Li Xiao, Xiaoxuan Xu, Fan Zhang, Ming Wang, Yan Xu, Dan Tang, Jiahui Wang, Yan Qin, Yu Liu, Chengyuan Tang, Liyu He, Anna Greka, Zhiguang Zhou, Fuyou Liu, Dong Zeng, Lin Sun Mitochondria play a crucial role in tubular injury in diabetic kidney disease (DKD). MitoQ is a mitochondria-targeted antioxidant that exerts protective effects in diabetic mice, but the mechanism underlying these effects is not clear. We demonstrated that mitochondrial abnormalities, such as defective mitophagy, mitochondrial reactive oxygen species (ROS) overexpressi...
Source: Redox Biology - December 20, 2016 Category: Biology Source Type: research
Induction of heme oxygenase-1 contributes to survival of Mycobacterium abscessus in human macrophages-like THP-1 cells
Publication date: Available online 20 January 2015 Source:Redox Biology Author(s): Maher Y. Abdalla , Iman M. Ahmad , Barbara Switzer , Bradley E. Britigan Mycobacterium abscessus (M.abs) is a rapidly growing mycobacterial species that infects macrophages, and is an important pathogen in patients with cystic fibrosis. We studied the early stages of M.abs infection of macrophages, with emphasis on the role of heme-oxygenase-1 (HO-1) in this infection. THP-1 cells were activated using TPA into macrophage-like cells and infected with M.abs for different time points. M.abs infection robustly induced HO-1 expression in the TH...
Source: Redox Biology - January 23, 2015 Category: Biology Source Type: research
AVE 0991 attenuates oxidative stress and neuronal apoptosis via Mas/PKA/CREB/UCP-2 pathway after subarachnoid hemorrhage in rats
In conclusion, our data provides evidence that Mas activation with AVE reduces oxidative stress injury and neuronal apoptosis through Mas/PKA/p-CREB/UCP-2 pathway after SAH. Furthermore, our study indicates that Mas may be a novel therapeutic treatment target in early brain injury of SAH.Graphical abstract
Source: Redox Biology - October 5, 2018 Category: Biology Source Type: research
Redox balance influences differentiation status of neuroblastoma in the presence of all-trans retinoic acid
In this study, the expression of a common neuronal differentiation marker [neurofilament M (NF-M)] in human SK-N-SH neuroblastoma cells treated with 10μM all-trans retinoic acid (ATRA) showed significantly increased expression in accordance with reduced cell number. This was accompanied by an increase in MitoSOX and DCFH2 oxidation that could be indicative of increased steady-state levels of reactive oxygen species (ROS) such as O2 •− and H2O2, which correlated with increased levels of MnSOD activity and immuno-reactive protein. Furthermore PEG-catalase inhibited the DCFH2 oxidation signal to a greater extent in the A...
Source: Redox Biology - December 8, 2015 Category: Biology Source Type: research
Advanced Oxidation Protein Products Sensitized the Transient Receptor Potential Vanilloid 1 via NADPH Oxidase 1 and 4 to Cause Mechanical Hyperalgesia
In conclusion, AOPPs increased significantly in CFA-induced hyperalgesia rats and they activated Nox1/Nox4-ROS to sensitize TRPV1-dependent Ca2+ influx and CGRP release which led to inducing mechanical hyperalgesia. Graphical abstract
Source: Redox Biology - September 17, 2016 Category: Biology Source Type: research
Autophagy regulates turnover of lipid droplets via ROS-dependent Rab25 activation in hepatic stellate cell
In this study, we sought to investigate the role of autophagy in the process of LD disappearance, and to further examine the underlying mechanisms in this molecular context. We found that LD disappearance during HSC activation was associated with a coordinate increase in autophagy. Inhibition or depletion of autophagy by Atg5 siRNA impaired LD disappearance of quiescent HSCs, and also restored lipocyte phenotype of activated HSCs. In contrast, induction of autophagy by Atg5 plasmid accelerated LD loss of quiescent HSCs. Importantly, our study also identified a crucial role for reactive oxygen species (ROS) in the facilitat...
Source: Redox Biology - December 21, 2016 Category: Biology Source Type: research
Acute telomerase components depletion triggers oxidative stress as an early event previous to telomeric shortening
Publication date: Available online 7 October 2017 Source:Redox Biology Author(s): José Santiago Ibáñez-Cabellos, Gisselle Pérez-Machado, Marta Seco-Cervera, Ester Berenguer-Pascual, José Luis García-Giménez, Federico V. Pallardó Loss of function of dyskerin (DKC1), NOP10 and TIN2 are responsible for different inheritance patterns of Dyskeratosis congenita (DC; ORPHA1775). They are key components of telomerase (DKC1 and NOP10) and shelterin (TIN2), and play an important role in telomere homeostasis. They participate in several fundamental cellular processes by contributing to Dyskeratosis congenita through mechanis...
Source: Redox Biology - October 7, 2017 Category: Biology Source Type: research
Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis
Publication date: June 2018 Source:Redox Biology, Volume 16 Author(s): Yachun Han, Xiaoxuan Xu, Chengyuan Tang, Peng Gao, Xianghui Chen, Xiaofen Xiong, Ming Yang, Shikun Yang, Xuejing Zhu, Shuguang Yuan, Fuyou Liu, Li Xiao, Yashpal S. Kanwar, Lin Sun NLRP3/IL-1β activation via thioredoxin (TRX)/thioredoxin-interacting protein (TXNIP) following mitochondria ROS (mtROS) overproduction plays a key role in inflammation. However, the involvement of this process in tubular damage in the kidneys of patients with diabetic nephropathy (DN) is unclear. Here, we demonstrated that mtROS overproduction is accompanied by decreases in ...
Source: Redox Biology - February 20, 2018 Category: Biology Source Type: research
Thioredoxin and glutaredoxin regulate metabolism through different multiplex thiol switches
Publication date: Available online 16 November 2018Source: Redox BiologyAuthor(s): MJ López-Grueso, González-Ojeda, R Requejo, McDonagh, CA Fuentes-Almagro, J Muntané, JA Bárcena, CA PadillaAbstractThe aim of the present study was to define the role of Trx and Grx on metabolic thiol redox regulation and identify their protein and metabolite targets. The hepatocarcinoma-derived HepG2 cell line under both normal and oxidative/nitrosative conditions by overexpression of NO synthase (NOS3) was used as experimental model. Grx1 or Trx1 silencing caused conspicuous changes in the redox proteome reflected by significant change...
Source: Redox Biology - November 17, 2018 Category: Biology Source Type: research
LRP1 activation attenuates white matter injury by modulating microglial polarization through Shc1/PI3K/Akt pathway after subarachnoid hemorrhage in rats
Publication date: Available online 23 January 2019Source: Redox BiologyAuthor(s): Jianhua Peng, Jinwei Pang, Lei Huang, Budbazar Enkhjargal, Tongyu Zhang, Jun Mo, Pei Wu, Weilin Xu, Yuchun Zuo, Jun Peng, Gang Zuo, Ligang Chen, Jiping Tang, John H. Zhang, Yong JiangAbstractWhite matter injury (WMI) is associated with motor deficits and cognitive dysfunctions in subarachnoid hemorrhage (SAH) patients. Therapeutic strategy targeting WMI would likely improve the neurological outcomes after SAH. Low-density lipoprotein receptor-related protein-1 (LRP1), a scavenger receptor of apolipoprotein E (apoE), is able to modulate microg...
Source: Redox Biology - January 24, 2019 Category: Biology Source Type: research
FoxO6-mediated IL-1β induces hepatic insulin resistance and age-related inflammation via the TF/PAR2 pathway in aging and diabetic mice
Publication date: Available online 3 April 2019Source: Redox BiologyAuthor(s): Dae Hyun Kim, Bonggi Lee, Jaewon Lee, Mi Eun Kim, Jun Sik Lee, Jae Heun Chung, Byung Pal Yu, H. Henry Dong, Hae Young ChungAbstractFoxO has been proposed to play a role in the promotion of insulin resistance, and inflammation. FoxO is a pro-inflammatory transcription factor that is a key mediator of generation of inflammatory cytokines such as IL-1β in the liver. However, the detailed association of FoxO6 with insulin resistance and age-related inflammation has not been fully documented. Here, we showed that FoxO6 was elevated in the livers of ...
Source: Redox Biology - April 4, 2019 Category: Biology Source Type: research
Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer
We examined the role of dihydrolipoamide dehydrogenase (DLD) in ferroptosis induction in head and neck cancer (HNC). The effects of cystine deprivation or sulfasalazine treatment and of DLD gene silencing/overexpression were tested on HNC cell lines and mouse tumor xenograft models. These effects were analyzed with regard to cell death, lipid reactive oxygen species (ROS) and mitochondrial iron production, mitochondrial membrane potential, mRNA/protein expression, and α-ketoglutarate dehydrogenase (KGDH)/succinate/aconitase activities. Cystine deprivation induced ferroptosis via glutaminolysis. Cystine deprivation or impo...
Source: Redox Biology - January 8, 2020 Category: Biology Source Type: research
Epalrestat increases intracellular glutathione levels in Schwann cells through transcription regulation
Publication date: 2014 Source:Redox Biology, Volume 2 Author(s): Keisuke Sato , Kaori Yama , Yu Murao , Ryosuke Tatsunami , Yoshiko Tampo Epalrestat (EPS), approved in Japan, is the only aldose reductase inhibitor that is currently available for the treatment of diabetic neuropathy. Here we report that EPS at near-plasma concentration increases the intracellular levels of glutathione (GSH), which is important for protection against oxidative injury, through transcription regulation. Treatment of Schwann cells with EPS caused a dramatic increase in intracellular GSH levels. EPS increased the mRNA levels of γ-glutamylcyst...
Source: Redox Biology - October 12, 2014 Category: Biology Source Type: research
Epalrestat increases glutathione, thioredoxin, and heme oxygenase-1 by stimulating Nrf2 pathway in endothelial cells
Publication date: Available online 10 December 2014 Source:Redox Biology Author(s): Kaori Yama , Keisuke Sato , Natsuki Abe , Yu Murao , Ryosuke Tatsunami , Yoshiko Tampo Epalrestat (EPS) is the only aldose reductase inhibitor that is currently available for the treatment of diabetic neuropathy. Recently, we found that EPS at near-plasma concentration increases the intracellular levels of glutathione (GSH) in rat Schwann cells. GSH plays a crucial role in protecting endothelial cells from oxidative stress, thereby preventing vascular diseases. Here we show that EPS increases GSH levels in not only Schwann cells but also ...
Source: Redox Biology - December 14, 2014 Category: Biology Source Type: research
