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Source: Molecular Immunology
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Total 48 results found since Jan 2013.
MYC inhibition increases PD-L1 expression induced by IFN-γ in hepatocellular carcinoma cells
This study implicates that a combination therapy targeting MYC function and PD-L1/PD-1 pathway might be effective for treatment of HCC.
Source: Molecular Immunology - July 10, 2018 Category: Allergy & Immunology Source Type: research
Targeted delivery of CD44s-siRNA by ScFv overcomes de novo resistance to cetuximab in triple negative breast cancer
In this study, we show that impaired EGFR degradation is crucial for resistance to cetuximab, which depends on the cell surface molecule CD44. To further investigate the role of CD44 in EGFR signaling and its treatment potential, we developed a targeting fusion protein composed of an anti-EGFR scFv generated from cetuximab and truncated protamine, called Ce-tP. CD44 siRNA can be specifically delivered into EGFR-positive TNBC cells by Ce-tP. Efficient knockdown of CD44 and suppression of both EGFR and downstream signaling by the Ce-tP/siRNA complex were observed in EGFR-positive TNBC cells. More importantly, our results als...
Source: Molecular Immunology - May 17, 2018 Category: Allergy & Immunology Source Type: research
Sub-lethal ultraviolet B irradiation and Poly I:C treatment synergistically induced apoptosis of HaCaT cells through NF- κB pathway
Publication date: July 2018 Source:Molecular Immunology, Volume 99 Author(s): Wuxiyar Otkur, Weiwei Liu, Jinda Wang, Xingfan Jia, Dianchao Huang, Fang Wang, Toshihiko Hayashi, Shin-ichi Tashiro, Satoshi Onodera, Takashi Ikejima Ultraviolet B (UVB) irradiation exerts multiple effects on skin cells, inducing apoptosis, senescence and carcinogenesis. Toll-like receptor 3, a member of pattern recognition receptors, is reported to initiate inflammation by recognizing double-strand RNA (dsRNA) released from UVB-irradiated cells. It has not been studied, however, whether apoptosis induction in UVB irradiation is attributed to TL...
Source: Molecular Immunology - April 18, 2018 Category: Allergy & Immunology Source Type: research
IRF1 up-regulates isg15 gene expression in dsRNA stimulation or CSFV infection by targeting nucleotides −487 to −325 in the 5′ flanking region
In this study, a sequence in the promoter region of the optineurin gene was compared to the 5′ flanking region of the porcine isg15 gene. Porcine IRF1 also possesses antiviral activity against several swine viruses (Li et al., 2015), but the mechanism is not well understood. Herein, we report that porcine IRF1 and ISG15 were up-regulated in porcine kidney (PK-15) cells following stimulation with double-stranded RNA (dsRNA) or classical swine fever virus (CSFV) infection. We also found that siRNA-mediated knockdown of IRF1 expression resulted in lower ISG15 expression in response to polyinosinic:polycytidylic acid [poly(I...
Source: Molecular Immunology - January 9, 2018 Category: Allergy & Immunology Source Type: research
Increased TRPC6 expression is associated with tubular epithelial cell proliferation and inflammation in diabetic nephropathy
Publication date: February 2018 Source:Molecular Immunology, Volume 94 Author(s): Yanqin Fu, Chongxian Wang, Dongming Zhang, Yaping Xin, Jun Li, Yuanyuan Zhang, Xiaojing Chu Although TRPC6 expression is shown to be significantly elevated in a rat model diabetic nephropathy (DN), its expression and role in human DN are unclear. We thus explored the role of TRPC6 in the pathophysiology of tubular epithelial cell injury following DN. HK-2 cells were cultured in a high-glucose medium to induce a DN cell model. Ad-TRPC6 and TRP6 siRNA were transfected to overexpress and knock down TRPC6. We found that TRPC6 expression was sign...
Source: Molecular Immunology - January 5, 2018 Category: Allergy & Immunology Source Type: research
Fatty acid oxidation contributes to IL-1 β secretion in M2 macrophages and promotes macrophage-mediated tumor cell migration
Publication date: February 2018 Source:Molecular Immunology, Volume 94 Author(s): Qi Zhang, Herui Wang, Chengyuan Mao, Mitchell Sun, Gifty Dominah, Liyuan Chen, Zhengping Zhuang Tumor-associated macrophages (TAMs) are predominantly M2 phenotype in solid cancers including hepatocellular carcinoma (HCC). Though differentiation of M2 macrophages has been recently linked to fatty acid oxidation (FAO), whether FAO plays a role in functional maintenance of M2 macrophages is still unclear. Here, we used an in vitro model to mimic TAM-HCC interaction in tumor microenvironment. We found that M2 monocyte-derived macrophages (MDMs) ...
Source: Molecular Immunology - December 15, 2017 Category: Allergy & Immunology Source Type: research
The protective effect of dexmedetomidine on LPS-induced acute lung injury through the HMGB1-mediated TLR4/NF- κB and PI3K/Akt/mTOR pathways
In conclusion, the present study showed that DEX exerted a protective effect on LPS-induced ALI rats likely through the HMGB1-mediated TLR4/NF-κB and PI3K/Akt/mTOR pathways.
Source: Molecular Immunology - December 12, 2017 Category: Allergy & Immunology Source Type: research
Irisin protects against neuronal injury induced by oxygen-glucose deprivation in part depends on the inhibition of ROS-NLRP3 inflammatory signaling pathway
Publication date: November 2017 Source:Molecular Immunology, Volume 91 Author(s): Juan Peng, Xian Deng, Wei Huang, Ji-hua Yu, Jian-xiong Wang, Jie-ping Wang, Shi-bin Yang, Xi Liu, Li Wang, Yun Zhang, Xiang-Yu Zhou, Hui Yang, Yan-Zheng He, Fang-yuan Xu Recent studies found that irisin, a newly discovered skeletal muscle-derived myokine during exercise, is also synthesized in various tissues of different species and protects against neuronal injury in cerebral ischemia. The NOD-like receptor pyrin 3 (NLRP3) inflammasome play an important role in detecting cellular damage and mediating inflammatory responses to aseptic tissu...
Source: Molecular Immunology - October 1, 2017 Category: Allergy & Immunology Source Type: research
Microsomal glutathione transferase 2 modulates LTC4 synthesis and ROS production in Apostichopus japonicus
Publication date: November 2017 Source:Molecular Immunology, Volume 91 Author(s): Zhen Zhang, Zhimeng Lv, Zhixin Wei, Chenghua Li, Yina Shao, Weiwei Zhang, Xuelin Zhao, Jinbo Xiong Microsomal glutathione transferase 2 (mGST2) is an integral membrane protein involved in detoxication of xenobiotics, and has also been suggested to catalyze the biosynthesis of pro-inflammatory mediator leukotriene C4 (LTC4) as homologous to LTC4 synthase (LTC4S) in mammals. In the present study, a novel mGST2 homology was identified from Apostichopus japonicus (designated as AjmGST2) by RACE approaches. The full-length cDNA of AjmGST2 was of ...
Source: Molecular Immunology - September 9, 2017 Category: Allergy & Immunology Source Type: research
Effect of lentivirus-mediated gene silencing, targeting toll-like receptor 2, on corneal allograft transplantation in rats
Conclusions LV mediated TLR2 siRNA could effectively down regulate the TLR2 expression via RNA interference and prolong the survival of corneal grafts, although not necessarily able to prevent the rejection.
Source: Molecular Immunology - September 8, 2017 Category: Allergy & Immunology Source Type: research
Hypoxia-induced ADAM 17 expression is mediated by RSK1-dependent C/EBP β activation in human lung fibroblasts
In this study, we investigated the roles that ribosomal S-6 kinase 1 (RSK1)/CCAAT/enhancer-binding protein β (C/EBPβ)-dependent ADAM 17 expression plays in hypoxia-induced CTGF expression in human lung fibroblasts. We observed that hypoxia caused increases in ADAM 17 expression and ADAM 17-luciferase activity in WI-38 cells. Hypoxia-induced CTGF-luciferase activity and CTGF expression were reduced in cells transfected with small interfering (si)RNA of ADAM 17 in WI-38 cells. Moreover, hypoxia-induced ADAM 17 expression was reduced by RSK1 siRNA and C/EBPβ siRNA. Hypoxia caused time-dependent increases in RSK1 phosphoryl...
Source: Molecular Immunology - June 21, 2017 Category: Allergy & Immunology Source Type: research
TLR2 mediates autophagy through ERK signaling pathway in Mycoplasma gallisepticum-infected RAW264.7 cells
In this study, we examined the typical hallmarks of autophagy and multiple signaling pathways by western blot, immunofluorescence microscopy and electron microscopy. The results indicated that infection of mouse macrophage cell line RAW264.7 with MG activated autophagy and mitogen-activated protein kinases (MAPKs). Silencing of TLR2 by siRNA substantially down-regulated MG-triggered autophagy in macrophages, and markedly reduced MG-induced extracellular regulated protein kinase (ERK) in macrophages but did not down-regulate c-Jun N-terminal kinase (JNK) and p38. Importantly, in macrophages, inhibition of ERK by PD98059 (ER...
Source: Molecular Immunology - May 4, 2017 Category: Allergy & Immunology Source Type: research
Interleukin-1 receptor associated kinase 1 is a potential therapeutic target of anti-inflammatory therapy for systemic lupus erythematosus
Publication date: July 2017 Source:Molecular Immunology, Volume 87 Author(s): Mingfang Li, Datang Yu, Bing Ni, Fei Hao Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disease and currently has no effective therapy. The genome-wide analyses indicate that interleukin-1 receptor associated kinase 1 (IRAK1) is associated with the susceptibility of SLE in humans. In the present study, we identified that IRAK1 was overexpressed and hyper-activated in splenic mononuclear cells from B6.MRL-Faslpr/Nju (B6.lpr) mice and peripheral blood mononuclear cells (PBMCs) from SLE patients. Intraperitoneal treatment w...
Source: Molecular Immunology - April 18, 2017 Category: Allergy & Immunology Source Type: research
TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF- κB/STAT3 pathways
In this study, we explored the role of TWEAK/Fn14 in activated human HSCs. The LX-2 cells were treated with TWEAK, and the expression of pro-inflammatory cytokines was assayed by enzyme-linked immunosorbent assay (ELISA) and real-time PCR (RT-PCR). Western blotting and RT-PCR were performed to evaluate the expression of Fn14 after TWEAK stimulation. Total and phosphorylated of inhibitor-κB (I-κB), nuclear factor kappa B (NF-κB), Janus kinase 2 (JAK2), and signal transducers and activators of transcription 3 (STAT3) were examined by western blotting after TWEAK stimulation and small interfering RNA (siRNA) transfection. ...
Source: Molecular Immunology - April 12, 2017 Category: Allergy & Immunology Source Type: research
