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Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research
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Total 69 results found since Jan 2013.
Ki-1/57 and CGI-55 ectopic expression impact cellular pathways involved in proliferation and stress response regulation
Publication date: December 2014 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1843, Issue 12 Author(s): Fernanda C. Costa , Ângela Saito , Kaliandra A. Gonçalves , Pedro M. Vidigal , Gabriela V. Meirelles , Gustavo C. Bressan , Jörg Kobarg Ki-1/57 (HABP4) and CGI-55 (SERBP1) are regulatory proteins and paralogs with 40.7% amino acid sequence identity and 67.4% similarity. Functionally, they have been implicated in the regulation of gene expression on both the transcriptional and mRNA metabolism levels. A link with tumorigenesis is suggested, since both paralogs show altered expression le...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 11, 2014 Category: Molecular Biology Source Type: research
Functional regulation of hypoxia inducible factor-1α by SET9 lysine methyltransferase
Publication date: Available online 27 January 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Qiong Liu , Hao Geng , Changhui Xue , Tomasz M. Beer , David Z. Qian HIF-1α is degraded by oxygen-dependent mechanisms but stabilized in hypoxia to form transcriptional complex HIF-1, which transactivates genes promoting cancer hallmarks. However, how HIF-1α is specifically regulated in hypoxia is poorly understood. Here, we report that the histone methyltransferase SET9 promotes HIF-1α protein stability in hypoxia and enhances HIF-1 mediated glycolytic gene transcription, thereby playing ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - January 29, 2015 Category: Molecular Biology Source Type: research
Homer proteins mediate the interaction between STIM1 and Cav1.2 channels
Publication date: Available online 21 February 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research Author(s): Natalia Dionisio , Tarik Smani , Geoffrey E. Woodard , Antonio Castellano , Gines M. Salido , Juan A. Rosado STIM1 is an ubiquitous Ca2+ sensor of the intracellular, agonist-sensitive, Ca2+ stores that communicates the filling state of the Ca2+ compartments to plasma membrane store-operated Ca2+ (SOC) channels. STIM1 has been presented as a point of convergence between store-operated and voltage-operated Ca2+ influx, both inducing activation of SOC channels while suppressing Cav1.2 channels....
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - February 22, 2015 Category: Molecular Biology Source Type: research
Hyper-dependence of breast cancer cell types on the nuclear transporter Importin β1
Publication date: August 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1853, Issue 8 Author(s): Henna V. Kuusisto , David A. Jans We previously reported that overexpression of members of the Importin (Imp) superfamily of nuclear transporters results in increased nuclear trafficking through conventional transport pathways in tumour cells. Here we show for the first time that the extent of overexpression of Impβ1 correlates with disease state in the MCF10 human breast tumour progression system. Excitingly, we find that targeting Impβ1 activity through siRNA is >30 times more eff...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - June 10, 2015 Category: Molecular Biology Source Type: research
DPF2 regulates OCT4 protein level and nuclear distribution
Publication date: December 2015 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1853, Issue 12 Author(s): Chao Liu, Dijuan Zhang, Yuxian Shen, Xiaofang Tao, Lihua Liu, Yongwang Zhong, Shengyun Fang The amount of transcription factor OCT4 is strictly regulated. A tight regulation of OCT4 levels is crucial for mammalian embryonic development and oncogenesis. However, the mechanisms underlying regulation of OCT4 protein expression and nuclear distribution are largely unknown. Here, we report that DPF2, a plant homeodomain (PHD) finger protein, is upregulated during H9 cell differentiation...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 22, 2015 Category: Molecular Biology Source Type: research
Dehydroepiandrosterone sulfate augments blood-brain barrier and tight junction protein expression in brain endothelial cells
Publication date: August 2017 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1864, Issue 8 Author(s): Dimitrios Papadopoulos, Georgios Scheiner-Bobis Tight junctions (TJ) between brain endothelial cells are essential for formation and maintenance of the blood-brain barrier (BBB). Although loss of BBB integrity is associated with several neuropathological disorders, treatments that augment or stabilise the BBB are scarce. Here we show that physiological concentrations of dehydroepiandrosterone sulfate (DHEAS) stimulate the expression of the TJ proteins zonula occludens-1 (ZO-1) and claudin-3 i...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - May 18, 2017 Category: Molecular Biology Source Type: research
Peptidyl-prolyl isomerase 1 (Pin1) preserves the phosphorylation state of tissue factor and prolongs its release within microvesicles
In conclusion, Pin1 is a fast-acting enzyme which may be utilised by cells to protect the phosphorylation state of TF in activated cells prolonging TF activity and release, and therefore ensuring adequate haemostasis. Graphical abstract
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research
Nerve/glial antigen (NG) 2 is a crucial regulator of intercellular adhesion molecule (ICAM)-1 expression
In this study, we demonstrate that in pericytes and glioblastoma cells intercellular adhesion molecule (ICAM)-1, an essential protein for leukocyte adhesion and transmigration, underlies a NG2-dependent expression. As shown by flow cytometry, Western blot analysis and quantitative real-time polymerase chain reaction (qRT-PCR), silencing of NG2 in human placenta-derived pericytes increased the expression of ICAM-1. Pathway analyses revealed that this is mediated by extracellular-regulated-kinases (ERK) 1/2 signaling. Moreover, leukocyte adhesion to NG2 siRNA-treated pericytes was significantly enhanced when compared to scra...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 2, 2017 Category: Molecular Biology Source Type: research
CRNDE, a long non-coding RNA responsive to insulin/IGF signaling, regulates genes involved in central metabolism
Publication date: February 2014 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1843, Issue 2 Author(s): Blake C. Ellis , Lloyd D. Graham , Peter L. Molloy Colorectal neoplasia differentially expressed (CRNDE) is a novel gene that is activated early in colorectal cancer but whose regulation and functions are unknown. CRNDE transcripts are recognized as long non-coding RNAs (lncRNAs), which potentially interact with chromatin-modifying complexes to regulate gene expression via epigenetic changes. Complex alternative splicing results in numerous transcripts from this gene, and we have identifie...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 1, 2014 Category: Molecular Biology Source Type: research
NADPH oxidase subunit p22phox-mediated reactive oxygen species contribute to angiogenesis and tumor growth through AKT and ERK1/2 signaling pathways in prostate cancer
In this study, we reported that levels of ROS and p22phox expression are greatly increased in human prostate cancer tissues, and knockdown of p22phox by specific small interfering RNA (siRNA) decreased ROS levels in prostate cancer cells. We also showed that stable downregulation of p22phox in prostate cancer cells inhibited cell proliferation and colony formation, which was mediated by AKT and extracellular signal-regulated kinase (ERK)1/2 signaling pathways and their downstream molecules hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF). The NADPH oxidase subunit NOX1 was also elevated ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 1, 2014 Category: Molecular Biology Source Type: research
Poly(ADP-ribose) polymerase 1 inhibition protects against low shear stress induced inflammation
Conclusions: LSS induced oxidative damage and PARP-1 activation via MEK/ERK pathway. PARP-1 inhibition restored Sirt1 activity by increasing NAD+ level and decreased iNOS and ICAM-1 expression by inhibiting NF-κB nuclear translocation and activity as well as NF-κB phosphorylation. PARP-1 played a fundamental role in LSS induced inflammation. Inhibition of PARP-1 might be a mechanism for treatment of inflammation response during atherosclerosis. Highlights ► We firstly demonstrated the critical role of PARP-1 in low shear stress induced inflammation. ► Low shear stress increased superoxide production and nitrotyrosine...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 1, 2014 Category: Molecular Biology Source Type: research
Involvement of store-operated Ca2+ entry in activation of AMP-activated protein kinase and stimulation of glucose uptake by M3 muscarinic acetylcholine receptors in human neuroblastoma cells
Publication date: December 2014 Source:Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Volume 1843, Issue 12 Author(s): Maria C. Olianas , Simona Dedoni , Pierluigi Onali Gq/11-coupled muscarinic acetylcholine receptors (mAChRs) belonging to M1, M3 and M5 subtypes have been shown to activate the metabolic sensor AMP-activated protein kinase (AMPK) through Ca2+/calmodulin-dependent protein kinase kinase-β (CaMKKβ)-mediated phosphorylation at Thr172. However, the source of Ca2+ required for this response has not been yet elucidated. Here, we investigated the involvement of store-operated Ca2+ entry (SOCE) ...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - November 11, 2014 Category: Molecular Biology Source Type: research
Novel role for TRPC4 in regulation of macroautophagy by a small molecule in vascular endothelial cells
In this study, we identified trans-3,5,4′-trimethoxystilbene (TMS) as a potent small molecule inducer of autophagy in human umbilical vascular endothelial cells (HUVECs) in the presence of serum. Using high-throughput DNA microarray and siRNA transfection technologies, we demonstrated that TMS induced autophagy by up-regulating the expression of the transient receptor potential canonical channel 4 (TRPC4), an important cation channel in HUVECs. In addition, the overexpression of TRPC4 by plasmid transfection also induced autophagy. Mechanistic studies revealed that the up-regulation of TRPC4 increased the intracellular C...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 4, 2014 Category: Molecular Biology Source Type: research
Downregulation of diacylglycerol kinase ζ enhances activation of cytokine-induced NF-κB signaling pathway
This study shows that siRNA-mediated DGKζ knockdown in HeLa cells facilitates degradation of IκB, followed by nuclear translocation of NF-κB p65 subunit. In addition, DGKζ-deficient MEFs show upregulation of p65 subunit phosphorylation at Serine 468 and 536 and its interaction with CBP transcriptional coactivator upon TNF-α stimulation. These modifications of p65 subunit might engender enhanced NF-κB transcriptional reporter assay of DGKζ knockdown cells. These findings provide further insight into the regulatory mechanisms of cytokine-induced NF-κB activation.
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 5, 2014 Category: Molecular Biology Source Type: research
CSN-associated USP48 confers stability to nuclear NF-κB/RelA by trimming K48-linked Ub-chains
In this study we observed that USP48, a DUB of hitherto ill-defined function identified through a siRNA screen, associates with the CSN and RelA in the nucleus. We show that USP48 trims rather than completely disassembles long K48-linked free and substrate-anchored Ub-chains, a catalytic property only shared with ataxin-3 (Atx3) and otubain-1 (OTU1), and that USP48 Ub-chain-trimming activity is regulated by casein-kinase-2 (CK2)-mediated phosphorylation in response to cytokine-stimulation. Functionally, we demonstrate for the first time the CSN and USP48 to cooperatively stabilize the nuclear pool of RelA, thereby facilita...
Source: Biochimica et Biophysica Acta (BBA) Molecular Cell Research - December 5, 2014 Category: Molecular Biology Source Type: research
