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Otx2 enhances transdifferentiation of M üller cells‐derived retinal stem cells into photoreceptor‐like cells
In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target f or gene therapy of retinal degenerative diseases.
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Yu Xiong, Hongpei Ji, Zhipeng You, Fei Yao, Rongrong Zhou, Weitao Song, Xiaobo Xia Tags: ORIGINAL ARTICLE Source Type: research

3D culture increases pluripotent gene expression in mesenchymal stem cells through relaxation of cytoskeleton tension
Abstract Three‐dimensional (3D) culture has been shown to improve pluripotent gene expression in mesenchymal stem cells (MSCs), but the underlining mechanisms were poorly understood. Here, we found that the relaxation of cytoskeleton tension of MSCs in 3D culture was critically associated with the expressional up‐regulation of Nanog. Cultured in spheroids, MSCs showed decreased integrin‐based cell–matrix adhesion but increased cadherin‐based cell–cell interaction. Different from that in 2D culture, where MSCs exhibited branched and multiple‐directed F‐actin stress bundles at the cell edge and strengthened s...
Source: Journal of Cellular and Molecular Medicine - February 28, 2017 Category: Molecular Biology Authors: Ying Zhou, Haiyan Chen, Hong Li, Yaojiong Wu Tags: Original Article Source Type: research

PPARγ silencing enhances osteogenic differentiation of human adipose‐derived mesenchymal stem cells
This study investigates the role of PPARγ in the lineage commitment of human adipose‐derived mesenchymal stem cells (hADSCs) by interfering with the function of PPARγ mRNA through small interfering RNAs (siRNAs) specific for PPARγ2. By applying an osteogenic induction condition less potent than that used conventionally, we found that PPARγ silencing led to retardation of adipogenesis and stimulated a higher level of matrix mineralization. The mRNA level of PPARγ decreased to 47% of control 2 days after treatment with 50 nmol/l PPARγ2 siRNA, while its protein expression was 60% of mock control. In the meantime, os...
Source: Journal of Cellular and Molecular Medicine - August 13, 2013 Category: Molecular Biology Authors: Mon‐Juan Lee, Hui‐Ting Chen, Mei‐Ling Ho, Chung‐Hwan Chen, Shu‐Chun Chuang, Sung‐Cheng Huang, Yin‐Chih Fu, Gwo‐Jaw Wang, Lin Kang, Je‐Ken Chang Tags: Short Communication Source Type: research