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Targeting YAP in malignant pleural mesothelioma
In this study, we evaluated the effect of targeting YAP in mesothelioma. First, we comprehensively studied YAP activity in five mesothelioma cell lines (211H, H2052, H290, MS‐1 and H2452) and one normal mesothelial cell line (LP9). We found decreased phospho‐YAP to YAP protein ratio and consistently increased GTIIC reporter activity in 211H, H2052 and H290 compared to LP9. The same three cell lines (IC50s < 1 μM) were more sensitive than LP9 (IC50 = 3.5 μM) to the YAP/TEAD inhibitor verteporfin. We also found that verteporfin significantly reduced YAP protein level, mRNA levels of YAP downstream genes and GTIIC re...
Source: Journal of Cellular and Molecular Medicine - May 4, 2017 Category: Molecular Biology Authors: Wen ‐Qian Zhang, Yu‐Yuan Dai, Ping‐Chih Hsu, Hui Wang, Li Cheng, Yi‐Lin Yang, Yu‐Cheng Wang, Zhi‐Dong Xu, Shu Liu, Geraldine Chan, Bin Hu, Hui Li, David M. Jablons, Liang You Tags: Original Article Source Type: research

Cul4A overexpression associated with Gli1 expression in malignant pleural mesothelioma
In this study, we analysed clinical mesothelioma tumours and found moderate to strong expression of Cul4A in 70.9% (51/72) of these tumours, as shown by immunohistochemistry. In 72.2% mesothelioma tumours with increased Cul4A copy number identified by fluorescence in situ hybridization analysis, Cul4A protein expression was moderate to strong. Similarly, Cul4A was overexpressed and Cul4A copy number was increased in human mesothelioma cell lines. Because Gli1 is highly expressed in human mesothelioma cells, we compared Cul4A and Gli1 expression in mesothelioma tumours and found their expression associated (P < 0.05, ...
Source: Journal of Cellular and Molecular Medicine - July 27, 2015 Category: Molecular Biology Authors: Yi‐Lin Yang, Jian Ni, Ping‐Chih Hsu, Jian‐Hua Mao, David Hsieh, Angela Xu, Geraldine Chan, Alfred Au, Zhidong Xu, David M. Jablons, Liang You Tags: Original Article Source Type: research