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Down ‐regulation of miR‐10a‐5p in synoviocytes contributes to TBX5‐controlled joint inflammation
Abstract MicroRNAs are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis (RA). The purpose of this study was to determine the relationship between miR‐10a‐5p and TBX5 in synoviocytes and evaluate their contribution to joint inflammation. The expression of miR‐10a‐5p and TBX5 in the synovium of RA and human synovial sarcoma cell line SW982 stimulated by IL‐1β was determined by RT‐qPCR and Western blotting. The direct interaction between miR‐10a‐5p and TBX5 3′UTR was determined by dual‐luciferase reporter assay in HeLa cells. Mimics and ...
Source: Journal of Cellular and Molecular Medicine - August 7, 2017 Category: Molecular Biology Authors: Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Xiaoying Wu, Manman Geng, Safdar Hussain, Yongsong Cai, Ke Xu, Peng Xu, Yan Han, Jian Sun, Liesu Meng, Shemin Lu Tags: Original Article Source Type: research

Role of protein arginine methyltransferase 5 in inflammation and migration of fibroblast ‐like synoviocytes in rheumatoid arthritis
Abstract To probe the role of protein arginine methyltransferase 5 (PRMT5) in regulating inflammation, cell proliferation, migration and invasion of fibroblast‐like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). FLSs were separated from synovial tissues (STs) from patients with RA and osteoarthritis (OA). An inhibitor of PRMT5 (EPZ015666) and short interference RNA (siRNA) against PRMT5 were used to inhibit PRMT5 expression. The standard of protein was measured by Western blot or immunofluorescence. The excretion and genetic expression of inflammatory factors were, respectively, estimated by enzyme‐l...
Source: Journal of Cellular and Molecular Medicine - October 31, 2016 Category: Molecular Biology Authors: Dongying Chen, Shan Zeng, Mingcheng Huang, Hanshi Xu, Liuqin Liang, Xiuyan Yang Tags: Original Article Source Type: research

Identification of C‐terminal Hsp70‐interacting protein as a mediator of tumour necrosis factor action in osteoblast differentiation by targeting osterix for degradation
Abstract In patients with inflammatory arthritis, tumour necrosis factor (TNF)‐α are overproduced in inflamed joints. This leads to local erosion of cartilage and bone, periarticular osteopenia, as well as osteoporosis. But less is known regarding the molecular mechanisms that mediate the effect of TNF‐α on osteoblast function. The purpose of this study was to test that C terminus of Hsc70‐interacting protein (CHIP) has a specific role in suppressing the osteogenic activity of osteoblasts under inflammatory conditions. C2C12, MC3T3‐E1 and HEK293T cell lines were cultured and cotransfected with related plasmids. A...
Source: Journal of Cellular and Molecular Medicine - March 26, 2015 Category: Molecular Biology Authors: Jianmin Xie, Jieruo Gu Tags: Original Article Source Type: research