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The mechanisms and significance of up‐regulation of RhoB expression by hypoxia and glucocorticoid in rat lung and A549 cells
In this study, we investigated the effects of hypoxia or/and GC on the expression and activition of RhoB in the lung of rats and human A549 lung carcinoma cells, and further studied its mechanism and significance. We found that hypoxia and dexamethasone (Dex), a synethic GC, not only significantly increased the expression and activation of RhoB independently but also coregulated the expresion of RhoB in vitro and in vivo. Up‐regulation of RhoB by hypoxia was in part through stabilizing the RhoB mRNA and protein. Inhibiting hypoxia‐activated hypoxia‐inducible transcription factor‐1α (HIF‐1α), c‐Jun N‐termina...
Source: Journal of Cellular and Molecular Medicine - February 24, 2016 Category: Molecular Biology Authors: Gao‐Xiang Huang, Xiao‐Yu Pan, Yi‐Duo Jin, Yan Wang, Xiao‐Lian Song, Chang‐Hui Wang, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

The mechanisms and significance of up ‐regulation of RhoB expression by hypoxia and glucocorticoid in rat lung and A549 cells
In this study, we investigated the effects of hypoxia or/and GC on the expression and activition of RhoB in the lung of rats and human A549 lung carcinoma cells, and further studied its mechanism and significance. We found that hypoxia and dexamethasone (Dex), a synethic GC, not only significantly increased the expression and activation of RhoB independently but also coregulated the expresion of RhoB in vitro and in vivo. Up‐regulation of RhoB by hypoxia was in part through stabilizing the RhoB mRNA and protein. Inhibiting hypoxia‐activated hypoxia‐inducible transcription factor‐1α (HIF‐1α), c‐Jun N‐termina...
Source: Journal of Cellular and Molecular Medicine - February 23, 2016 Category: Molecular Biology Authors: Gao ‐Xiang Huang, Xiao‐Yu Pan, Yi‐Duo Jin, Yan Wang, Xiao‐Lian Song, Chang‐Hui Wang, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

Overexpression of deubiquitinating enzyme USP28 promoted non‐small cell lung cancer growth
Abstract Non‐small cell lung cancer (NSCLC) accounts for most lung cancer. To develop new therapy required the elucidation of NSCLC pathogenesis. The deubiquitinating enzymes USP 28 has been identified and studied in colon and breast carcinomas. However, the role of USP28 in NSCLC is unknown. The level mRNA or protein level of USP28 were measured by qRT‐PCR or immunohistochemistry (IHC). The role of USP28 in patient survival was revealed by Kaplan–Meier plot of overall survival in NSCLC patients. USP28 was up or down regulated by overexpression plasmid or siRNA transfection. Cell proliferation and apoptosis was assay...
Source: Journal of Cellular and Molecular Medicine - February 5, 2015 Category: Molecular Biology Authors: Lei Zhang, Biao Xu, Yong Qiang, Hairong Huang, Changtian Wang, Demin Li, Jianjun Qian Tags: Original Article Source Type: research