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Silencing of MUC8 by siRNA increases P2Y2-induced airway inflammation.
Abstract Mucin hypersecretion and overproduction are frequent manifestations of respiratory disease. Determining the physiological function of airway mucin is presently considered more important than identifying the relevant signaling pathways. The lack of a full-length human MUC8 cDNA sequence has hindered the generation of a Muc8 knockout mouse line. Thus, the precise physiological functions of MUC8 are unclear. Herein, we investigated the function of MUC8 using a siRNA-mediated genetic silencing approach in human airway epithelial cells. Herein, intracellular IL-1α production was stimulated by an ATP/P2Y2 comp...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 9, 2015 Category: Cytology Authors: Cha HJ, Jung MS, Ahn DW, Choi JK, Ock MS, Kim KS, Yoon JH, Song EJ, Song KS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Passive siRNA transfection method for gene knockdown in air-liquid interface airway epithelial cell cultures
Am J Physiol Lung Cell Mol Physiol. 2021 May 26. doi: 10.1152/ajplung.00122.2021. Online ahead of print.ABSTRACTDifferentiation of human bronchial epithelial cells (HBEs) in air-liquid interface (ALI) cultures recapitulates organotypic modeling of the in vivo environment. Although ALI cultures are invaluable for studying the respiratory epithelial barrier, loss-of-function studies are limited by potentially cytotoxic reagents in classical transfection methods, the length of the differentiation protocol, and the number of primary epithelial cell passages. Here, we present the efficacy and utility of a simple method for siRN...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - May 26, 2021 Category: Cytology Authors: Colleen M Bartman Kimberly E Stelzig David R Linden Y S Prakash Sergio E Chiarella Source Type: research

Potential Role of Cartilage Oligomeric Matrix Protein in the Modulation of Pulmonary Arterial Smooth Muscle Superoxide by Hypoxia.
Abstract Changes in reactive oxygen species and extracellular matrix seem to participate in pulmonary hypertension development. Since we recently reported evidence for chronic hypoxia decreasing expression of cartilage oligomeric matrix protein (COMP) and evidence for this controlling loss of pulmonary arterial smooth muscle BMP receptor-2 (BMPR2) and contractile phenotype proteins, we examined if changes in superoxide metabolism could be an important factor in a bovine pulmonary artery (BPA), organoid cultured under hypoxia for 48hrs model. Hypoxia (3% oxygen) caused a depletion of COMP in BPA, but not in bovine ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - August 6, 2019 Category: Cytology Authors: Yu H, Alruwaili N, Hu B, Kelly MR, Zhang B, Sun D, Wolin MS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Blockade of endothelial, but not epithelial, cell expression of pd-l1 following severe shock attenuates the development of indirect acute lung injury in mice.
This study sets out to establish the comparative contribution of PD-L1 expression by pulmonary endothelial cells (ECs) and/or epithelial cells (EpiCs) to the development of indirect acute lung injury (iALI) by taking advantage of the observation that treatment with naked siRNA by intra-tracheal (I.T.) delivery in mice primarily effects lung EpiCs, but not lung ECs; while intravenous (I.V.) delivery of liposomal encapsulated siRNA largely targets vascular ECs including the lung, but not pulmonary EpiCs. We showed that, using a mouse model of iALI (induced by hemorrhage followed by septic challenge [Hem-CLP]), PD-L1 expressi...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 28, 2020 Category: Cytology Authors: Xu S, Yang Q, Bai J, Tao T, Tang L, Chen Y, Chung CS, Fallon EA, Ayala A Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Endothelin-1 Depletion of Cartilage Oligomeric Matrix Protein Modulates Pulmonary Artery Superoxide and Iron Metabolism-associated Mitochondrial Heme Biosynthesis
This study examines if heme biosynthesis associated iron metabolism is regulated in pulmonary arteries by endothelin-1 (ET1) potentially through modulating Cartilage Oligomeric Matrix Protein (COMP) availability. Our studies in organoid cultured endothelium-rubbed bovine pulmonary arteries (BPA) observed COMP depletion by siRNA or hypoxia increases NOX2 and superoxide, and depletes mitochondrial SOD2. ET1 also increases superoxide in a manner that potentially impairs mitochondrial heme biosynthesis. In this study, organoid culture of BPA with ET1 (10nM) increases superoxide in the mitochondrial matrix and extra mitochondri...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - August 9, 2022 Category: Cytology Authors: Hang Yu Norah Alruwaili Melissa R Kelly Bin Zhang Aijing Liu Yingqi Wang Dong Sun Michael S Wolin Source Type: research

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