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APC functions at the centrosome to stimulate microtubule growth.
Abstract The adenomatous polyposis coli (APC) tumor suppressor is multi-functional. APC is known to localize at the centrosome, and in mitotic cells contributes to formation of the mitotic spindle. To test whether APC contributes to nascent microtubule (MT) growth at interphase centrosomes, we employed MT regrowth assays in U2OS cells to measure MT assembly before and after nocodazole treatment and release. We showed that siRNA knockdown of full-length APC delayed both initial MT aster formation and MT elongation/regrowth. In contrast, APC-mutant SW480 cancer cells displayed a defect in MT regrowth that was unaffe...
Source: The International Journal of Biochemistry and Cell Biology - November 7, 2015 Category: Biochemistry Authors: Lui C, Ashton C, Sharma M, Brocardo MG, Henderson BR Tags: Int J Biochem Cell Biol Source Type: research