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Increased expression of platelet-derived growth factor associated protein-1 is associated with PDGF-B mediated glioma progression.
This study aimed to characterize the role of PDAP-1 in PDGF-mediated glioma proliferation. The expression of PDAP-1 was observed to be significantly increased (p<0.05) in grade IV glioma tissue and cell lines compared to grade III. siRNA-mediated knockdown of PDAP-1 reduced the expression of PDGF-B and its downstream genes (Akt1/Protein kinase B (PKB) and phosphoinositide-dependent kinase-1 (PDK1) by up to 50%. In PDAP-1 knockdown glioma cells, more than a twofold reduction was also observed in the level of phosphorylated Akt. Interestingly, knockdown of PDAP-1 in combination with PDGF-B antibody inhibited glioma cell p...
Source: The International Journal of Biochemistry and Cell Biology - July 18, 2016 Category: Biochemistry Authors: Sharma VK, Singh A, Srivastava SK, Kumar V, Gardi NL, Nalwa A, Dinda AK, Chattopadhyay P, Yadav S Tags: Int J Biochem Cell Biol Source Type: research

G9a inhibition induced PKM2 regulates autophagic responses.
Abstract Epigenetic regulation by histone methyltransferase G9a is known to control autophagic responses. As the link between autophagy and metabolic homeostasis is widely accepted, we investigated whether G9a affects metabolic circuitries to affect autophagic response in glioma cells. Both pharmacological inhibition and siRNA mediated knockdown of G9a increased autophagy marker LC3B in glioma cells. G9a inhibitor BIX-01294 (BIX) induced Akt-dependent increase in HIF-1α expression and activity. Inhibition of Akt-HIF-1α axis reversed BIX-mediated (i) increase in LC3B expression and (ii) decrease in Yes-associated...
Source: The International Journal of Biochemistry and Cell Biology - July 10, 2016 Category: Biochemistry Authors: Ahmad F, Dixit D, Joshi SD, Sen E Tags: Int J Biochem Cell Biol Source Type: research