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Novel Interaction of Antioxidant-1 with TRAF4: Role in Inflammatory Responses in Endothelial Cells.
We reported that in proinflammatory cytokine TNFa-stimulated endothelial cells (ECs), cytosolic Cu chaperone Atox1 functions as a Cu-dependent transcription factor for NOX organizer p47phox, thereby increasing ROS-dependent inflammatory gene expression. However, the role and mechanism of Atox1 nuclear translocation in inflamed ECs remain unclear. Using enface staining and nuclear fractionation, here we show that Atox1 was localized in the nucleus in inflamed aortas from ApoE-/- mice with Angiotensin II infusion on a high fat diet, while it was found in cytosol in those from control mice. In cultured human ECs, TNFα stimul...
Source: Am J Physiol Cell Ph... - September 24, 2019 Category: Cytology Authors: Das A, Sudhahar V, Ushio-Fukai M, Fukai T Tags: Am J Physiol Cell Physiol Source Type: research

Copper Transporter ATP7A Interacts with IQGAP1, a Rac1 Binding Scaffold Protein: Role in PDGF-induced VSMC Migration and Vascular Remodeling.
Abstract Vascular smooth muscle cell (VSMC) migration contributes to neointimal formation after vascular injury. We previously demonstrated that copper (Cu) transporter ATP7A is involved in PDGF-induced VSMC migration in Cu- and Rac1-dependent manner. Underlying mechanism is still unknown. Here we show that ATP7A interacts with IQGAP1, a Rac1- and receptor tyrosine kinase-binding scaffold proteins, which mediates PDGF-induced VSMC migration and vascular remodeling. In cultured rat aortic SMCs, PDGF stimulation rapidly promoted ATP7A association with IQGAP1 and Rac1, and their translocation to lipid rafts and leadi...
Source: Am J Physiol Cell Ph... - September 26, 2018 Category: Cytology Authors: Ashino T, Kohno T, Sudhahar V, Ash D, Ushio-Fukai M, Fukai T Tags: Am J Physiol Cell Physiol Source Type: research