• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

Linarin Protects the Kidney against Ischemia/Reperfusion Injury via the Inhibition of Bioactive ETS2/IL-12.
Abstract Ischemia/reperfusion injury (IRI), a participant in acute kidney injury (AKI), can occur as a series of pathological processes such as inflammation. Linarin (LIN) has been widely used for different diseases. To confirm the anti-inflammatory value and relevant mechanism of LIN during IRI, in vivo and vitro models were established. LIN or dissolvent was given, and histologic analysis, quantitative (q)RT-PCR, serum creatinine and blood urea nitrogen testing were used to evaluate kidney injury. Microarray analysis, protein-protein interaction (PPI) analysis and molecular docking were used to identify the targ...
Source: Biological and Pharmaceutical Bulletin - January 6, 2021 Category: Drugs & Pharmacology Authors: Chengyu Y, Long Z, Bin Z, Hong L, Xuefei S, Congjuan L, Caixia C, Yan X Tags: Biol Pharm Bull Source Type: research

Yi-Zhi-Fang-Dai Formula Exerts a Protective Effect on the Injury of Tight Junction Scaffold Proteins in Vitro and in Vivo by Mediating Autophagy through Regulation of the RAGE/CaMKK β/AMPK/mTOR Pathway.
This study aimed to explore the underlying mechanism of YZFDF in alleviating the injury of TJ proteins. We examined the impacts of YZFDF on autophagy-related proteins and the histopathology of Aβ in the APP/PS1 double-transgenic male mice. We then performed the free intracellular calcium levels [Ca2+]i analysis and the cognitive behavior test of the AD model. Our results showed that YZFDF ameliorated the injury of TJ proteins by reducing the mRNA transcription and expression of the receptor for advanced glycation end-products (RAGE), the levels of [Ca2+]i, calmodulin-dependent protein kinase β (CaMKKβ), phosphorylated A...
Source: Biological and Pharmaceutical Bulletin - December 4, 2020 Category: Drugs & Pharmacology Authors: Chan Y, Chen W, Chen Y, Lv Z, Wan W, Li Y, Zhang C Tags: Biol Pharm Bull Source Type: research

Involvement of CD73 and A2B receptor in radiation-induced DNA damage response and cell migration in human glioblastoma A172 cells.
Abstract Glioblastoma is the most common malignant tumor of the central nervous system and is treated with a combination of surgery, radiation and chemotherapy. However, the tumor often acquires radiation resistance, which is characterized by an increased DNA damage response (DDR). Here, we show that CD73, which generates extracellular adenosine from ATP, and A2B receptor, which is activated by adenosine, are involved in the γ-radiation-induced DDR and the enhanced migration ability of human glioblastoma cell line A172. To investigate DDR, we evaluated ATM activation and focus formation of γH2AX and 53BP1 in the...
Source: Biological and Pharmaceutical Bulletin - December 1, 2020 Category: Drugs & Pharmacology Authors: Kitabatake K, Kaji T, Tsukimoto M Tags: Biol Pharm Bull Source Type: research

β-Estradiol Enhanced Secretion of Lipoprotein Lipase from Mouse Mammary Tumor FM3A Cells.
Abstract The role of β-estradiol (E2) in lipoprotein metabolism in mammary tumors is unclear, therefore, we investigated the effect of E2 on the secretion of lipoprotein lipase (LPL) from mouse mammary tumor FM3A cells. E2-treated cells increased the secretion of active LPL from FM3A cells in a time- and dose-dependent manner. Activity of mitogen-activated protein kinase (MAPK) was increased in the tumor cells treated with E2, and enhanced secretion of LPL was suppressed by MAPK kinase 1/2 inhibitor, PD98059, extracellular signal-regulated kinase (ERK) 1/2 inhibitor, FR180204, p38 MAPK inhibitor, SB202190, and ph...
Source: Biological and Pharmaceutical Bulletin - September 5, 2020 Category: Drugs & Pharmacology Authors: Fujii T, Ogasawara M, Kamishikiryo J, Morita T Tags: Biol Pharm Bull Source Type: research

Sinomenine inhibited Interleukin-1 β-induced matrix metalloproteinases levels via SOCS3 up-regulation in SW1353 cells.
Sinomenine inhibited Interleukin-1β-induced matrix metalloproteinases levels via SOCS3 up-regulation in SW1353 cells. Biol Pharm Bull. 2020 Sep 02;: Authors: Qi W, Gu Y, Wang Z, Fan W Abstract Matrix metalloproteinases (MMPs) are required for collagen degradation which play a key pathological role in arthritis progression. Herein, the effect of sinomenine (SN) on Interleukin 1 beta (IL-1β)-induced MMPs production and its underlying mechanism were explored in SW1353 cells. The MTT assay showed that 200 and 400 µM SN significantly inhibited SW1353 cell proliferation, thus the lower dose of SN (25-100...
Source: Biological and Pharmaceutical Bulletin - September 1, 2020 Category: Drugs & Pharmacology Authors: Qi W, Gu Y, Wang Z, Fan W Tags: Biol Pharm Bull Source Type: research

GDP-Bound Rab27a Dissociates from the Endocytic Machinery in a Phosphorylation-Dependent Manner after Insulin Secretion.
Abstract Glucose-stimulated insulin secretion is controlled by both exocytosis and endocytosis in pancreatic β-cells. Although endocytosis is a fundamental step to maintain cellular responses to the secretagogue, the molecular mechanism of endocytosis remains poorly defined. We have previously shown that in response to high concentrations of glucose, guanosine 5'-diphosphate (GDP)-bound Rab27a is recruited to the plasma membrane where IQ motif-containing guanosine 5'-triphosphatase (GTPase)-activating protein 1 (IQGAP1) is expressed, and that complex formation promotes endocytosis of secretory membranes after ins...
Source: Biological and Pharmaceutical Bulletin - September 3, 2019 Category: Drugs & Pharmacology Authors: Kimura T, Yamaoka M, Terabayashi T, Kaibuchi K, Ishikawa T, Ishizaki T Tags: Biol Pharm Bull Source Type: research

Reciprocal Regulation of Chaperone-Mediated Autophagy/Microautophagy and Exosome Release.
Abstract Autophagy-lysosome proteolysis is involved in protein quality control and classified into macroautophagy (MA), microautophagy (mA) and chaperone-mediated autophagy (CMA), by the routes of substrate delivery to lysosomes. Both autophagy-lysosome proteolysis and exosome release are strongly associated with membrane trafficking. In the present study, we investigated how chemical and small interfering RNA (siRNA)-mediated activation and inhibition of these autophagic pathways affect exosome release in AD293 cells. Activation of MA and mA by rapamycin and activation of CMA by mycophenolic acid significantly de...
Source: Biological and Pharmaceutical Bulletin - August 3, 2019 Category: Drugs & Pharmacology Authors: Oshima M, Seki T, Kurauchi Y, Hisatsune A, Katsuki H Tags: Biol Pharm Bull Source Type: research

Anti-RelA siRNA-Encapsulated Flexible Liposome with Tight Junction-Opening Peptide as a Non-invasive Topical Therapeutic for Atopic Dermatitis.
Abstract Small interfering RNA (siRNA) has been proposed as a novel treatment for atopic dermatitis (AD) because it suppresses sequence-specific mRNA expression. Indeed siRNA-based therapy achieves an almost complete cure with fewer side effects than currently available treatments. However, the tight junctions in the granular layer of the epidermis in the atopic skin are barriers to siRNA delivery. We previously reported the potential clinical utility of AT1002, a peptide that opens tight junctions. In the present study, we evaluated a topical siRNA-based therapy for AD using AT1002 in combination with a flexible ...
Source: Biological and Pharmaceutical Bulletin - July 3, 2019 Category: Drugs & Pharmacology Authors: Ibaraki H, Kanazawa T, Kurano T, Oogi C, Takashima Y, Seta Y Tags: Biol Pharm Bull Source Type: research

BubR1 Is Essential for Thio-Dimethylarsinic Acid-Induced Spindle Assembly Checkpoint and Mitotic Cell Death for Preventing the Accumulation of Abnormal Cells.
In this study, we showed that thio-DMA promotes the phosphorylation of BubR1 protein, which is one of the constituents of the spindle assembly checkpoint (SAC) complex and accumulates in the cell in mitotic phase. Binding of Mad2 to CDC20, also known as the marker of the mitotic checkpoint complex (MCC) formation during the activation of SAC, was enhanced and mitotic associated cell death by apoptosis was promoted in HeLa cells but not in HepG2 cells. Basal BubR1 protein level in HepG2 was 10-times lower than that of HeLa cells. Consequently, BubR1 knockdown HeLa cells were generated by small interfering RNA (siRNA) techni...
Source: Biological and Pharmaceutical Bulletin - July 3, 2019 Category: Drugs & Pharmacology Authors: Kita K, Imai Y, Asaka N, Suzuki T, Ochi T Tags: Biol Pharm Bull Source Type: research

VEGF antagonism attenuates cerebral ischemia/reperfusion-induced injury via inhibiting endoplasmic reticulum stress-mediated apoptosis.
Abstract Endoplasmic reticulum (ER) stress-mediated apoptosis pathway is considered to play a vital role in mediating stroke and other cerebrovascular diseases. Previous studies have showed that vascular endothelial growth factor (VEGF) antagonism reduced cerebral ischemic/reperfusion (CI/R) damage, but whether attenuation of ER stress-induced apoptosis is contributing to its mechanisms remains elusive. Our study aimed to investigate the protective effect of VEGF antagonism on CI/R-induced injury. First, oxygen-glucose deprivation and re-oxygenation (OGD/R) BEND3 cell model was constructed to estimate siRNA-VEGF o...
Source: Biological and Pharmaceutical Bulletin - March 1, 2019 Category: Drugs & Pharmacology Authors: Feng SQ, Zong SY, Liu JX, Chen Y, Xu R, Yin X, Zhao R, Li Y, Luo TT Tags: Biol Pharm Bull Source Type: research

Shikonin Suppresses Lymphangiogenesis via NF- κB/HIF-1α Axis Inhibition.
In conclusion, shikonin inhibits lymphangiogenesis in vitro by interfering the NF-κB/HIF-1α pathway and involves in suppression of VEGF-C and VEGFR-3 mRNA expression. PMID: 30381665 [PubMed - in process]
Source: Biological and Pharmaceutical Bulletin - November 2, 2018 Category: Drugs & Pharmacology Authors: Prangsaengtong O, Jantaree P, Lirdprapamongkol K, Svasti J, Koizumi K Tags: Biol Pharm Bull Source Type: research