• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

A Feedback Loop between TGF- β1 and < em > ATG5 < /em > Mediated by miR-122-5p Regulates Fibrosis and EMT in Human Trabecular Meshwork Cells
Curr Issues Mol Biol. 2023 Mar 13;45(3):2381-2392. doi: 10.3390/cimb45030154.ABSTRACTAutophagy is a cell's evolutionary conserved process for degrading and recycling cellular proteins and removing damaged organelles. There has been an increasing interest in identifying the basic cellular mechanism of autophagy and its implications in health and illness during the last decade. Many proteinopathies such as Alzheimer's and Huntington's disease are reported to be associated with impaired autophagy. The functional significance of autophagy in exfoliation syndrome/exfoliation glaucoma (XFS/XFG), remains unknown though it is pres...
Source: Mol Biol Cell - March 28, 2023 Category: Molecular Biology Authors: Munmun Chakraborthy Aparna Rao Source Type: research

CRISPR-Cas9 mediated genome editing of Huntington's disease neurospheres
CONCLUSION: Our study confirmed that CAG repeat of R6/2 mouse-derived neurospheres can be edited through CRISPR-Cas9. Editing of CAG repeat sequence decreases polyglutamine aggregation and cellular apoptosis of HD neurospheres, which may be related to the increased expressions of PGC-1α and BDNF. Our data provide the evidence that CRISPR-Cas9 mediated genome editing has therapeutic potential on HD neuronal cells.PMID:36550260 | DOI:10.1007/s11033-022-08175-6
Source: Mol Biol Cell - December 22, 2022 Category: Molecular Biology Authors: Ji Yun Han Jaewoo Seo Yoori Choi Wooseok Im Jae-Jun Ban Jung-Joon Sung Source Type: research

Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture.
Abstract Determining the molecular mechanisms that contribute to trinucleotide repeat (TNR) expansions is essential to understanding the origin of genetically inherited diseases, such as Huntington's disease, and to inform efforts in developing therapeutic treatments. As one resource to probe the mechanisms of TNR expansions, we describe an expansion assay in human tissue culture cells. The cell line SVG-A, derived from human astrocytes, has the important property of supporting expansions in culture, unlike many cell lines derived from patients. SVG-A cells are also amenable to standard genetic and biochemical tec...
Source: Mol Biol Cell - October 7, 2019 Category: Molecular Biology Authors: Williams GM, Lahue RS Tags: Methods Mol Biol Source Type: research