• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

1063 Topically applied NanoFlares to measure gene expression in vivo: Proof-of-concept
Spherical nucleic acids (SNAs) are topical agents that penetrate human skin to knock down genes via antisense and siRNA pathways. NanoFlares, SNAs that release sequence-specific fluorescence when binding to intracellular mRNA targets, measure mRNA levels in live cells. We hypothesized that NanoFlares could be topically applied to measure gene expression in vivo. As proof-of-concept, we detected connective tissue growth factor (CTGF), known to be increased in hypertrophic scars and keloids. Using fibroblasts from normal skin, keloids, hypertrophic scars and peri-scar tissue, we found that: a) NanoFlares are specific; b) exp...
Source: Journal of Investigative Dermatology - April 27, 2018 Category: Dermatology Authors: A.S. Paller, D. Yeo, C. Wiraja, C. Mirkin, C. Xu Tags: Pharmacology and Drug Development Source Type: research

Transforming growth factor-beta inducible early gene-1 (TIEG1) represses Smad7-mediated activation of TGF- β1/Smad signaling in keloid pathogenesis
Transforming growth factor β (TGF-β)/Smad signaling plays a key role in excessive fibrosis and keloid formations. Smad7 is a negative feedback regulator that prevents activation of TGF-β/Smad signaling. However, the regulatory mechanism for Smad7 in the keloid pathogenic process remains elusive. Here, we show that expressi on of TGF-β inducible early gene-1 (TIEG1) is markedly higher in keloid fibroblasts (KFs), while protein, mRNA, and promoter activity levels of Smad7 are decreased. When TIEG1 was knocked down with small interfering RNA (siRNA), both the promoter activity and protein expression of Smad7 were increa s...
Source: Journal of Investigative Dermatology - January 16, 2017 Category: Dermatology Authors: Zhi-Cheng Hu, Fen Shi, Peng Liu, Jian Zhang, Dong Guo, Xiao-ling Cao, Chu-Fen Chen, Shanqiang Qu, Jia-Yuan Zhu, Bing Tang Tags: Original Article Source Type: research