• Filter By:
• Specialty
• Source
• Condition
• Cancer
• Infectious Disease
• Drug
• Training
• Management
• Procedure
• Therapy
• Vaccine
• Nutrition
• Country

PARP14 regulates EP4 receptor expression in human colon cancer HCA-7  cells
Biochem Biophys Res Commun. 2022 Jul 19;623:133-139. doi: 10.1016/j.bbrc.2022.07.055. Online ahead of print.ABSTRACTE type prostanoid 4 (EP4) receptors and their signaling pathways have been implicated in the development and malignant transformation of colorectal cancer. We herein demonstrated that the mono(ADP-ribosyl)ation of histone deacetylase (HDAC)1 and HDAC2 by poly(ADP-ribose) polymerase 14 (PARP14) may be required to induce the expression of EP4 receptors. The suppression of PARP14 activity by siRNA and/or its inhibitors reduced the mRNA expression of EP4 receptors. Thus, the expression of their proteins to approx...
Source: Biochemical and Biophysical Research communications - August 1, 2022 Category: Biochemistry Authors: Masato Mashimo Asuka Shimizu Aimi Mori Ayaka Hamaguchi Keijo Fukushima Naofumi Seira Takeshi Fujii Hiromichi Fujino Source Type: research

Chaperone-mediated autophagy regulates apoptosis and the proliferation of colon carcinoma cells.
In this study, we investigated CMA activity in tissue specimens from CRC patients and mouse models of colitis-associated CRC (induced by administration of AOM plus DSS). In addition, we down-regulated CMA in CT26 colon carcinoma cells stably transfected with a vector expressing a siRNA targeting LAMP-2A, the limiting component in the CMA pathway, to explore the role of CMA in these cells. Apoptosis was detected using TUNEL assay, and the apoptosis-related proteins were detected using western blotting. Cell proliferation was assessed using MTT assay, Ki-67 labelling and western blotting for PCNA. We found that LAMP-2A expre...
Source: Biochemical and Biophysical Research communications - November 20, 2019 Category: Biochemistry Authors: Peng JQ, Han SM, Chen ZH, Yang J, Pei YQ, Bao C, Qiao L, Chen WQ, Liu B Tags: Biochem Biophys Res Commun Source Type: research

Induction of Trop-2 expression through the binding of galectin-3 to MUC1.
Abstract Both mucin 1 (MUC1) and trophoblast cell surface antigen 2 (Trop-2) are overexpressed in various epithelial tumor cells, and their high expression is correlated with a poor prognosis. Both proteins were expressed in a human breast cancer cell line, MCF-7 cells, but neither was in a human colon cancer cell line, HCT116 cells. When MUC1 cDNA was introduced into HCT116 cells (HCT116/MUC1), expression of Trop-2 was induced. Reciprocally, treatment of MCF-7 cells with MUC1 siRNA reduced the level of Trop-2. Mithramycin A, an inhibitor of specificity protein 1 (Sp1) transcription factor, effectively inh...
Source: Biochemical and Biophysical Research communications - June 9, 2019 Category: Biochemistry Authors: Yamashita T, Mori Y, Alzaaqi SM, Yashiro M, Sawada T, Hirakawa K, Nakada H Tags: Biochem Biophys Res Commun Source Type: research

Sulindac sulfone inhibits the mTORC1 pathway in colon cancer cells by directly targeting voltage-dependent anion channel 1 and 2.
Abstract Sulindac sulfone is a metabolite of sulindac, a non-steroidal anti-inflammatory drug (NSAID), without anti-inflammatory ability. However, sulindac sulfone has been reported to significantly reduce polyps in patients with colorectal adenomatous polyposis in clinical trials. Thus, sulindac sulfone is expected to be useful for the chemoprevention of neoplasia with few side effects related to anti-inflammatory ability. To date, the molecular targets of sulindac sulfone have not yet fully investigated. Therefore, in order to newly identify sulindac sulfone-binding proteins, we generated sulindac sulfone-fixed ...
Source: Biochemical and Biophysical Research communications - October 13, 2018 Category: Biochemistry Authors: Aono Y, Horinaka M, Iizumi Y, Watanabe M, Taniguchi T, Yasuda S, Sakai T Tags: Biochem Biophys Res Commun Source Type: research

miR-3120-5p promotes colon cancer stem cell stemness and invasiveness through targeting Axin2.
In this study, we used colon cancer cell lines HCT-116 and SW-480 to investigate the effects of miR-3120-5p on stemness and invasiveness of colon cancer. We found that the population of CD133 + and Lgr5+ stem cells in both cell lines expressed miR-3120-5p highly, and introducing miR-3120-5p into both cell lines increased the population of cancer stem cells, as measured by flow cytometry, qRT-PCR and sphere formation assays. Transwell assay, Gelatin zymography assay and Western blot assays further revealed that miR-3120-5p promotes colon cancer cells invasive ability. By the target prediction algorithm TargetScan, we foun...
Source: Biochemical and Biophysical Research communications - January 4, 2018 Category: Biochemistry Authors: Hongdan L, Feng L Tags: Biochem Biophys Res Commun Source Type: research

Snail transcription factor NLS and importin β1 regulate the subcellular localization of Cathepsin L and Cux1.
Abstract Several recent studies have highlighted an additional unexpected localization and site of action for Cathepsin L (Cat L) protease within the nucleus in breast, colon and prostate cancer, however, its role in the nucleus was unclear. It was proposed to mediate proteolytic processing of the transcription factor CCAAT-displacement protein/cut homeobox transcription factor (Cux1) from the full-length p200 isoform to generate the p110 and p90 isoforms, of which the p110 isoform was shown to act as a cell cycle regulator to accelerate entry into the S phase. The p110 isoform has also been shown to bind to the p...
Source: Biochemical and Biophysical Research communications - July 8, 2017 Category: Biochemistry Authors: Burton LJ, Henderson V, Liburd L, Odero-Marah VA Tags: Biochem Biophys Res Commun Source Type: research

MAPK13 is preferentially expressed in gynecological cancer stem cells and has a role in the tumor-initiation.
In this study, we analyzed the gene expression profiles of gynecological CSCs/CICs isolated as aldehyde dehydrogenase high (ALDH(high)) cells, and found that MAPK13, PTTG1IP, CAPN1 and UBQLN2 were preferentially expressed in CSCs/CICs. MAPK13 is expressed in uterine, ovary, stomach, colon, liver and kidney cancer tissues at higher levels compared with adjacent normal tissues. MAPK13 gene knockdown using siRNA reduced the ALDH(high) population and abrogated the tumor-initiating ability. These results indicate that MAPK13 is expressed in gynecological CSCs/CICs and has roles in the maintenance of CSCs/CICs and tumor-initiati...
Source: Biochemical and Biophysical Research communications - March 8, 2016 Category: Biochemistry Authors: Yasuda K, Yoshihiko H, Kuroda T, Takaya A, Kubo T, Kanaseki T, Tsukahara T, Hasegawa T, Saito T, Sato N, Torigoe T Tags: Biochem Biophys Res Commun Source Type: research

The small molecule survivin inhibitior YM155 may be an effective treatment modality for colon cancer through increasing apoptosis.
In this study, we investigated the roles of CD133 and survivin expression in colon cancer biology in vitro and comparatively analyzed the anticancer effects of survivin inhibitor on CD133(+) cells (ctrl-siRNA) and small interfering RNA (siRNA)-induced CD133(-) cells (CD133-siRNA) obtained from a single colon cancer cell line. CD133 knockdown via siRNA transfection did not change the tumorigenicity of cells, although in vitro survivin expression levels in CD133(+) cells were higher than those in siRNA-induced CD133(-) cells. The transfection procedure seemed to induce survivin expression. Notably, a significant number of CD...
Source: Biochemical and Biophysical Research communications - February 5, 2016 Category: Biochemistry Authors: Li WL, Lee MR, Cho MY Tags: Biochem Biophys Res Commun Source Type: research

Chemoresistance of CD133(+) colon cancer may be related with increased survivin expression.
Abstract CD133, putative cancer stem cell marker, deemed to aid chemoresistance. However, this claim has been challenged recently and we previously reported that patients with CD133(+) colon cancer have benefit from 5-fluorouracil (5-FU) chemotherapy incontrast to no benefit in patients with CD133(-) cancer. To elucidate the role of CD133 expression in chemoresistance, we silenced the CD133 expression in a colon cancer cell line and determined its effect on the biological characteristics downstream. We comparatively analyzed the sequential changes of MDR1, ABCG2, AKT1 and survivin expression and the result of prol...
Source: Biochemical and Biophysical Research communications - May 19, 2015 Category: Biochemistry Authors: Lee MR, Ji SY, Mia-Jan K, Cho MY Tags: Biochem Biophys Res Commun Source Type: research