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Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma
CONCLUSION: Our study shows that TMOD3 may be associated with the progression of PAAD cells, and that it is an independent risk factor for poor pathological features and prognosis of PAAD. It may be helpful as a prognostic indicator of clinical outcomes in PAAD patients.PMID:37563820 | DOI:10.2174/1386207326666230810142646
Source: Combinatorial Chemistry and High Throughput Screening - August 11, 2023 Category: Chemistry Authors: Bin Zhong Dan-Dan Ma Tao Zhang Qi Gong Y I Dong Jian-Xin Zhang Zhong-Hu Li Wei-Dong Jin Source Type: research

High-throughput Single-cell Mass Spectrometry Reveals Abnormal Lipid Metabolism in Pancreatic Ductal Adenocarcinoma
Angew Chem Int Ed Engl. 2021 Sep 10. doi: 10.1002/anie.202107223. Online ahead of print.ABSTRACTEven populations of clonal cells are heterogeneous, which requires high-throughput analysis methods with single-cell sensitivity. Here, we propose a rapid, label-free single-cell analytical method based on active capillary dielectric barrier discharge ionization mass spectrometry, which can analyze multiple metabolites in single cells at a rate of 38 cells/minute. Multiple cell types (HEK-293T, PANC-1, CFPAC-1, H6c7, HeLa and iBAs) were discriminated successfully. We found that abnormal lipid metabolism occurs in pancreatic canc...
Source: Angewandte Chemie - September 10, 2021 Category: Chemistry Authors: Qinlei Liu Wenjie Ge Tongtong Wang Jiayi Lan Sandra Mart ínez-Jarquín Christian Wolfrum Markus Stoffel Renato Zenobi Source Type: research

Arachidonate 12-lipoxygenase and 12-hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer Molecular Bases of Disease
In this study, we investigated whether the chronological aging of normal human fibroblasts (NHFs), a previously underappreciated area in pancreatic cancer research, influences the progression and therapeutic outcomes of PDAC. Results from experiments with murine xenografts and 2D and 3D co-cultures of NHFs and PDAC cells revealed that older NHFs stimulate proliferation of and confer resistance to radiation therapy of PDAC. MS-based metabolite analysis indicated that older NHFs have significantly increased arachidonic acid 12-lipoxygenase (ALOX12) expression and elevated levels of its mitogenic metabolite, 12-(S)-hydroxy-5,...
Source: Journal of Biological Chemistry - May 14, 2020 Category: Chemistry Authors: Ehab H. Sarsour, Jyung Mean Son, Amanda L. Kalen, Wusheng Xiao, Juan Du, Matthew S. Alexander, Brianne R. O'Leary, Joseph J. Cullen, Prabhat C. Goswami Tags: Cell Biology Source Type: research

Regulation and related mechanism of GSN mRNA level by hnRNPK in lung adenocarcinoma cells.
Authors: Liu XH, Ma J, Feng JX, Zhang YF, Liu L Abstract Gelsolin (GSN) is an actin filament-capping protein that plays a key role in cell migration. Here we showed that heterogeneous nuclear ribonucleoprotein K (hnRNPK) regulates GSN expression level by binding to the 3'-untranslated region (3'UTR) of GSN mRNA in non-small cell lung cancers (NSCLC) H1299 cells which are highly metastatic and express high level of GSN. We found that hnRNPK overexpression increased the mRNA and protein level of GSN, whereas hnRNPK knockdown by siRNA decreased the mRNA and protein level of GSN in both H1299 and A549 cells, indicating...
Source: Biological Chemistry - February 18, 2019 Category: Chemistry Tags: Biol Chem Source Type: research

Involvement of Notch1 signaling in malignant progression of A549 cells subjected to prolonged cadmium exposure Signal Transduction
Cadmium exposure is known to increase lung cancer risk, but the underlying molecular mechanisms in cadmium-stimulated progression of malignancy are unclear. Here, we examined the effects of prolonged cadmium exposure on the malignant progression of A549 human lung adenocarcinoma cells and the roles of Notch1, hypoxia-inducible factor 1α (HIF-1α), and insulin-like growth factor 1 receptor (IGF-1R)/Akt/extracellular signal-regulated kinase (ERK)/p70 S6 kinase 1 (S6K1) signaling pathways. Exposing A549 cells to 10 or 20 μm cadmium chloride (CdCl2) for 9–15 weeks induced a high proliferative potential, the epithelial-mese...
Source: Journal of Biological Chemistry - May 12, 2017 Category: Chemistry Authors: Kota Fujiki, Hisako Inamura, Takamitsu Miyayama, Masato Matsuoka Tags: Cell Biology Source Type: research

Dual Role of Nrf2 in Arsenic Carcinogenesis Cell Biology
Arsenic (As3+) is a carcinogen with considerable environmental and occupational relevancy. The present study shows that As3+-transformed human lung bronchial epithelial BEAS-2B cells (AsT cells) exhibit the property of apoptosis resistance. The level of basal reactive oxygen species (ROS) is very low in AsT cells in correlation with elevated expressions of both antioxidant enzymes and antiapoptotic proteins. Nuclear factor erythroid 2-related factor (Nrf2) and p62 are constitutively expressed. These two proteins up-regulate antioxidant enzymes and antiapoptotic proteins. The knockdown of Nrf2 or p62 by small interfering RN...
Source: Journal of Biological Chemistry - November 6, 2015 Category: Chemistry Authors: Son, Y.-O., Pratheeshkumar, P., Roy, R. V., Hitron, J. A., Wang, L., Divya, S. P., Xu, M., Luo, J., Chen, G., Zhang, Z., Shi, X. Tags: Molecular Bases of Disease Source Type: research