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Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide- α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway
Acta Biochim Biophys Sin (Shanghai). 2021 Sep 8:gmab115. doi: 10.1093/abbs/gmab115. Online ahead of print.ABSTRACTPhotodynamic therapy (PDT), which is a new method for treating tumors, has been used in the treatment of cancer. In-depth research has shown that PDT cannot completely kill tumor cells, indicating that tumor cells are resistant to PDT. Glucose regulatory protein 78 (GRP78), which is a key regulator of endoplasmic reticulum stress, has been confirmed to be related to tumor resistance and recurrence, but there are relatively few studies on the further mechanism of GRP78 in PDT. Our experiment aimed to observe the...
Source: Acta Biochimica et Biophysica Sinica - September 8, 2021 Category: Biochemistry Authors: Qiang Zuo Yunsheng Ou Shenxi Zhong Haoyang Yu Fangbiao Zhan Muzi Zhang Source Type: research

miR-487a performs oncogenic functions in osteosarcoma by targeting BTG2 mRNA.
In this study, we elucidated the involvement of miR-487a in OS and the underlying molecular mechanisms. We found that miR-487a was upregulated in OS clinical samples and cell lines. Knockdown of miR-487a suppressed OS cell growth and invasion and induced apoptosis; however, overexpression of miR-487a promoted OS cell growth and invasion. Accordingly, downregulation of miR-487a significantly suppressed tumor growth of OS xenografts in vivo. Furthermore, B-cell translocation gene 2 (BTG2) mRNA was found to be a novel target of miR-487a. Knockdown of BTG2 using small interfering RNA (siRNA) recapitulated the oncogenic effects...
Source: Acta Biochimica et Biophysica Sinica - May 12, 2020 Category: Biochemistry Authors: Gu Z, Wu S, Xu G, Wu W, Mao B, Zhao S Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

Proliferation inhibition and apoptosis promotion by dual silencing of VEGF and Survivin in human osteosarcoma.
Abstract Simultaneous silencing of multiple upregulated genes is an attractive and viable treatment strategy for many incurable diseases including cancer. Herein we used a dual gene-targeted siRNA conjugate composed of VEGF and Survivin siRNA sequences in the same backbone to inhibit proliferation and angiogenesis in two human osteosarcoma cell lines. We synthesized siRNA sequences targeting the VEGF and Survivin genes individually (VEGF siRNA and Survivin siRNA) or simultaneously (one-chain-double-target siRNA: dual siRNA). VEGF and Survivin mRNA and protein expression levels in human osteosarcoma MG-63 and Saos-...
Source: Acta Biochimica et Biophysica Sinica - December 19, 2018 Category: Biochemistry Authors: Gu J, Ji Z, Li D, Dong Q Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research