Filtered By:
Specialty: Cytology
This page shows you your search results in order of relevance.
Order by Relevance | Date
Total 80202 results found since Jan 2013.
Resetting of the 24-nt siRNA landscape in rice zygotes
Genome Res. 2021 Dec 23:gr.275981.121. doi: 10.1101/gr.275981.121. Online ahead of print.ABSTRACTThe zygote, a totipotent stem cell, is crucial to the life cycle of sexually reproducing organisms. It is produced by the fusion of two differentiated cells - the egg and sperm, which in plants have radically different siRNA transcriptomes from each other, and from multicellular embryos. Due to technical challenges, the epigenetic changes that accompany the transition from differentiated gametes to totipotent zygote are poorly understood. Since siRNAs serve as both regulators and outputs of the epigenome, we performed here the ...
Source: Cell Research - December 24, 2021 Category: Cytology Authors: Chenxin Li Jonathan I Gent Hengping Xu Hong Fu Scott D Russell Venkatesan Sundaresan Source Type: research
PAMAM versus PEI complexation for siRNA delivery: interaction with model lipid membranes and cellular uptake
CONCLUSION: Complexation of PAMAM dendrimer or bPEI with siRNA modified physicochemical properties of the parent polymer, however it did not impact on the mechanism of interaction with model lipid bilayers or how the polymer/siRNA complex interacted and was internalized by HeLa cells. Interaction of siRNA polymer complexes with cells is related to the action of the parent polymer.PMID:35318566 | DOI:10.1007/s11095-022-03229-7
Source: Cell Research - March 23, 2022 Category: Cytology Authors: Patrick K C Chang Clive A Prestidge Kristen E Bremmell Source Type: research
Cetuximab-siRNA Conjugate Linked Through Cationized Gelatin Knocks Down KRAS G12C Mutation in NSCLC Sensitizing the Cells Toward Gefitinib
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211041453. doi: 10.1177/15330338211041453.ABSTRACTDelivery of small-interfering RNA (siRNA) has been of great interest in the past decade for effective gene silencing. To overcome synthetic and regulatory challenges posed by nanoparticle-mediated siRNA delivery, antibody-siRNA conjugate (ARC) platform is emerging as a potential siRNA delivery system suitable for clinical translation. Herein, we have developed a delivery technology based on the ARC platform for stable delivery of siRNA called as Gelatin-Antibody Delivery System (GADS). In GADS, positively charged gelatin act...
Source: Cell Research - September 20, 2021 Category: Cytology Authors: K Sreedurgalakshmi R Srikar K Harikrishnan Lakshmi Srinivasan Reena Rajkumari Source Type: research
Clinical pharmacology of siRNA therapeutics: current status and future prospects
Expert Rev Clin Pharmacol. 2022 Oct 17. doi: 10.1080/17512433.2022.2136166. Online ahead of print.ABSTRACTINTRODUCTION: Small interfering RNA (siRNA) has emerged as a powerful tool for post-transcriptional downregulation of multiple genes for various therapies. Naked siRNA molecules are surrounded by several barriers that tackle their optimum delivery to target tissues such as limited cellular uptake, short circulation time, degradation by endonucleases, glomerular filtration, and capturing by the reticuloendothelial system (RES).AREAS COVERED: This review provides insights into studies that investigate various siRNA-based...
Source: Cell Research - October 17, 2022 Category: Cytology Authors: Ahmed Khaled Abosalha Jacqueline Boyajian Waqar Ahmad Paromita Islam Merry Ghebretatios Sabrina Schaly Rahul Thareja Karan Arora Satya Prakash Source Type: research
Multilevel chitosan-gelatin particles loaded with P4HA1 siRNA suppress glioma development
Drug Deliv Transl Res. 2023 Sep 4. doi: 10.1007/s13346-023-01422-8. Online ahead of print.ABSTRACTIt has been reported that prolyl 4-hydroxylase subunit alpha 1 (P4HA1) promoted tumor growth and metastasis of glioma; thus, targeting P4HA1 may be a promising therapeutic strategy against glioma. In consideration of the instability of siRNA in vivo, the chitosan-gelatin microspheres loaded with P4HA1 siRNA (P4HA1 siRNA@CGM) were employed. Firstly, the gel electrophoresis and hemolytic test were performed to assess the stability and blood compatibility of P4HA1 siRNA@CGM. Then, methyl thiazolyl tetrazolium (MTT), cell colony f...
Source: Cell Research - September 4, 2023 Category: Cytology Authors: Yiting Zhou Jiajia Tian Yi Zhu Yating Zhang Xudong Zhao Source Type: research
Development of hyaluronic acid-anchored polycaprolactone nanoparticles for efficient delivery of PLK1 siRNA to breast cancer
Drug Deliv Transl Res. 2023 Jan 15. doi: 10.1007/s13346-022-01288-2. Online ahead of print.ABSTRACTPlK1 has a significant role in the development of breast cancer. Thus, silencing of PlK1 gene may arrest the growth of breast cancer. However, the in vivo stability of PlK1 siRNA after injection remains a challenge to target the specific site. The delivery of siPlK1 RNA via viral vector and amine group-terminated dendrimer is associated with immune reaction and cellular cytotoxicity. Thus, in the present study, hyaluronic acid-functionalized and -thiolated polycaprolactone nanoparticles (SH-HPP NPs) were developed for enhanci...
Source: Cell Research - January 14, 2023 Category: Cytology Authors: Dolly Jain Awesh K Yadav Source Type: research
Recombinant Adenovirus siRNA Knocking Down the Ndufs4 Gene Alleviates Myocardial Apoptosis Induced by Oxidative Stress Injury
In conclusion, we successfully constructed Ndufs4 siRNA recombinant adenovirus; furthermore, the downexpression of the Ndufs4 gene may alleviate H2O2-induced H9c2 cell apoptosis.PMID:36741296 | PMC:PMC9897913 | DOI:10.1155/2023/8141129
Source: Cell Research - February 6, 2023 Category: Cytology Authors: Beibei Wang Jinsheng Zhang Aijun Xu Source Type: research
MC3/SAINT-O-Somes, a novel liposomal delivery system for efficient and safe delivery of siRNA into endothelial cells
In this study, we describe the design and characterisation of two liposomal siRNA delivery systems formulated with the cationic MC3 lipid or MC3/SAINT mixed lipids, referred to as MC3-O-Somes (MOS) and MC3/SAINT-O-Somes (MSS). The two formulations showed comparable physicochemical properties, except for better siRNA encapsulation efficiency in the MSS formulation. Antibody-mediated VCAM-1 targeting (AbVCAM-1) increased the association of the targeted MOS and MSS with activated EC, although the targeted MOS showed a significantly higher VCAM-1 specific association than the targeted MSS. AbVCAM-1 MSS containing RelA siRNA ac...
Source: Cell Research - March 15, 2023 Category: Cytology Authors: Yutong He Mees Barlag Jos ée A Plantinga Grietje Molema Jan A A M Kamps Source Type: research
Combination of B7H6-siRNA and temozolomide synergistically reduces stemness and migration properties of glioblastoma cancer cells
This study aimed to understand the potential role and molecular mechanism of the combination therapy of B7H6-siRNA and temozolomide in glioblastoma cancer. U87 cells were treated with B7H6-siRNA and temozolomide, separately and in combination. Cell viability, stemness, cell migration, and apoptosis were measured. The results of this work presented the synergistic effect of B7H6-siRNA and temozolomide in inhibiting the cancerous features of the U87 cell line. Down-regulating B7H6-siRNA expression inhibited the cell viability of U87 glioblastoma cancer cells and increased their sensitivity to temozolomide. In addition, a not...
Source: Experimental Cell Research - May 29, 2023 Category: Cytology Authors: Nadia Allahyarzadeh Khiabani Mohammad Amin Doustvandi Fateme Mohammadnejad Elnaz Salmani Hassan Kohal Neda Boushehri Mahdi Jafarlou Behzad Baradaran Source Type: research
Central role of c-Src in NOX5- mediated redox signaling in vascular smooth muscle cells in human hypertension
CONCLUSIONS: We define NOX5/ROS/c-Src as a novel feedforward signaling network in human VSMCs. Amplification of this system in hypertension contributes to VSMC dysfunction. Dampening the NOX5/ROS/c-Src pathway may ameliorate hypertension-associated vascular injury.TRANSLATIONAL PERSPECTIVE: Oxidative stress is a major factor contributing to vascular damage in hypertension. We corroborate experimental evidence that NOX-derived ROS generation is increased in human vascular smooth muscle cells (VSMC) and demonstrate that in human hypertension NOX5 upregulation is a major trigger of VSMC dysfunction. We uncover new regulatory ...
Source: Cell Research - July 28, 2021 Category: Cytology Authors: Livia L Camargo Augusto C Montezano Misbah Hussain Yu Wang Zhiguo Zou Francisco J Rios Karla B Neves Rheure Alves-Lopes Fazli R Awan Tomasz J Guzik Thomas Jensen Richard C Hartley Rhian M Touyz Source Type: research
The effects of miR-26b-5p on fibroblast-like synovial cells in rheumatoid arthritis (RA-FLS) via targeting EZH2
CONCLUSION: MiR-26b-5p may affect the biological characteristics of RA-FLS via targeting EZH2, including proliferation, apoptosis, invasion and migration, as well as the secretion of cytokines, thus playing a potential therapeutic role in RA.PMID:34325277 | DOI:10.1016/j.tice.2021.101591
Source: Tissue and Cell - July 29, 2021 Category: Cytology Authors: Pei Hu Zheng-Sen Dong Shuang Zheng Xin Guan Lei Zhang Lin Li Zhen Liu Source Type: research
