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Preparation and Application of Monoclonal Antibodies for an Immune Complex Transfer Enzyme Immunoassay of an Egg White Allergen, Lipocalin-Type Prostaglandin D Synthase
In this study, we prepared four monoclonal antibodies against chicken L-PGDS and developed a sandwich enzyme-linked immunosorbent assay (ELISA) and a highly sensitive immune complex transfer enzyme immunoassay (ICT-EIA) to quantify L-PGDS in hen egg whites. The detection sensitivity of ICT-EIA for L-PGDS (0.01 ng/mL) was 2,000-fold higher than that of ELISA, which could not be adapted to determine the amount of L-PGDS in egg white. Thus, ICT-EIA is a better method for quantification of trace allergens and expected to be applied to the quantification of other food allergens. Hen eggs (white-shelled eggs from Julia Lite hens...
Source: Journal of Nutritional Science and Vitaminology - April 30, 2023 Category: Nutrition Authors: Satoshi Numata Hao Luo Makiko Suzuki Source Type: research
