An upstream signaling gene calmodulin regulates the synthesis of insect wax via activating fatty acid biosynthesis pathway
Insect Biochem Mol Biol. 2024 Apr 23:104126. doi: 10.1016/j.ibmb.2024.104126. Online ahead of print.ABSTRACTInsect wax accumulates on the surface of insect cuticle, which acts as an important protective barrier against rain, ultraviolet light radiation, pathogens, etc. The waxing behavior, wax composition and molecular mechanism underling wax biosynthesis are unclear in dustywings. Herein, the current study determined the vital developmental stage for waxing behavior in dustywings, examined the components of waxy secretions, and identified key regulatory genes for wax biosynthesis. The wax glands were mainly located on the...
Source: Insect Biochemistry and Molecular Biology - April 25, 2024 Category: Biochemistry Authors: Min Li Shuo Yan Xinying Feng Qinhong Jiang Mei Guan Jie Shen Zhiqi Liu Source Type: research

Metabolomics analysis of larval secretions reveals a caste-driven nutritional shift in a social wasp colony
Insect Biochem Mol Biol. 2024 Apr 22:104128. doi: 10.1016/j.ibmb.2024.104128. Online ahead of print.ABSTRACTSocial wasps exhibit a unique nutritional cycle in which adults feed larvae with prey, and larvae provide adults with larval secretions (LS). LS serves as a vital nutritional source for adults, contributing to the colony's health and reproductive success. The LS nutrient composition has been previously reported in various wasp species, yet these analyses focused solely on worker-destined larvae, overlooking the potential caste designation effects on LS composition. Using metabolomics techniques, we analysed and compa...
Source: Insect Biochemistry and Molecular Biology - April 24, 2024 Category: Biochemistry Authors: Levona Bodner Weronika Jasinska Sofia Bouchebti Inon Scharf Yariv Brotman Eran Levin Source Type: research

A novel target-site mutation (H146Q) outside the ubiquinone binding site of succinate dehydrogenase confers high levels of resistance to cyflumetofen and pyflubumide in Tetranychus urticae
In this study a new mutation, H146Q in a highly conserved region of subunit B of complex II, was identified in a T. urticae population resistant to all METI-IIs. In contrast to previously described mutations, H146Q is located outside the ubiquinone binding site of complex II. Marker-assisted backcrossing of this mutation in a susceptible genetic background validated its association with resistance to cyflumetofen and pyflubumide, but not cyenopyrafen or cyetpyrafen. Biochemical assays and the construction of inhibition curves with isolated mitochondria corroborated this selectivity. In addition, phenotypic effects of H146Q...
Source: Insect Biochemistry and Molecular Biology - April 24, 2024 Category: Biochemistry Authors: Emre İnak Sander De Rouck Berke Demirci Wannes Dermauw Sven Geibel Thomas Van Leeuwen Source Type: research

BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research

BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research

BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research

BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research

BmNPV p35 regulates apoptosis in Bombyx mori via a novel target of interaction with the BmVDAC2-BmRACK1 complex
In this study, the interaction between BmVDAC2 and BmRACK1 in the mitochondria was determined by various methods. We found that BmNPV p35 interacts directly with BmVDAC2 rather than BmRACK1. BmNPV infection significantly reduced the expression of BmVDAC2, and activated the mitochondrial apoptosis pathway. Overexpression of BmVDAC2 in BmN cells inhibited BmNPV-induced cytochrome c (cyto c) release, decrease in mitochondrial membrane potential as well as apoptosis. Additionally, the inhibition of cyto c release by BmVDAC2 requires the involvement of BmRACK1 and protein kinase C. Interestingly, overexpression of p35 inhibited...
Source: Insect Biochemistry and Molecular Biology - April 14, 2024 Category: Biochemistry Authors: Lin-Bao Zhu Han-Dan Zhu Zhi-Hao Huang Hui-Hua Cao Sadaf Ayaz Jia-Yue Yang Xi-Ya Chen Ying Zhang Shi-Huo Liu Jia-Ping Xu Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research

Decoding the genome of bloodsucking midge Forcipomyia taiwana (Diptera: Ceratopogonidae): Insights into odorant receptor expansion
In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.PMID:38570118 | DOI:10.1016/j.ibmb.2024.104115 (Source: Insect Biochemistry and Molecular Biology)
Source: Insect Biochemistry and Molecular Biology - April 3, 2024 Category: Biochemistry Authors: Ming-Der Lin Chia-Hsien Chuang Chih-Hsin Kao Shu-Hwa Chen Szu-Chieh Wang Ping-Heng Hsieh Guan-Yu Chen Chun-Chia Mao Jeng-Yi Li Mei-Yeh Jade Lu Chung-Yen Lin Source Type: research