Insect Biochemistry and Molecular Biology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Fibroin heavy chain gene replacement with a highly ordered synthetic repeat sequence in Bombyx mori
Insect Biochem Mol Biol. 2023 Aug 30;161:104002. doi: 10.1016/j.ibmb.2023.104002. Online ahead of print.ABSTRACTThe exceptional quality of silkworm silk is attributed to the amino acid sequence of its fibroin heavy chain (Fib-H) protein. The large central domain of Fib-H, which consists of glycine- and alanine-rich crystalline regions interspersed with amorphous motifs of approximately 30 amino acid residues, is considered crucial for fibrilization and determines the properties of the silk fiber. We established a technical platform to modify the Fib-H core region systematically using transcription activator-like effector n...
Source: Insect Biochemistry and Molecular Biology - September 1, 2023 Category: Biochemistry Authors: Yoko Takasu Nobuto Yamada Katsura Kojima Masatoshi Iga Fumiko Yukuhiro Tetsuya Iizuka Taiyo Yoshioka Source Type: research

The Wnt pathway regulates wing morph determination in Acyrthosiphon pisum
Insect Biochem Mol Biol. 2023 Aug 30:104003. doi: 10.1016/j.ibmb.2023.104003. Online ahead of print.ABSTRACTWing dimorphism occurs in insects as a survival strategy to adapt to environmental changes. In response to environmental cues, mother aphids transmit signals to their offspring, and the offspring either emerge as winged adults or develop as wingless adults with degeneration of the wing primordia in the early instar stage. However, how the wing morph is determined in the early instar stage is still unclear. Here, we established a surgical sampling method to obtain precise wing primordium tissues for transcriptome anal...
Source: Insect Biochemistry and Molecular Biology - September 1, 2023 Category: Biochemistry Authors: Ping Zhou Xin Zong Shuo Yan Junzheng Zhang Dan Wang Jie Shen Source Type: research

Fibroin heavy chain gene replacement with a highly ordered synthetic repeat sequence in Bombyx mori
Insect Biochem Mol Biol. 2023 Aug 30:104002. doi: 10.1016/j.ibmb.2023.104002. Online ahead of print.ABSTRACTThe exceptional quality of silkworm silk is attributed to the amino acid sequence of its fibroin heavy chain (Fib-H) protein. The large central domain of Fib-H, which consists of glycine- and alanine-rich crystalline regions interspersed with amorphous motifs of approximately 30 amino acid residues, is considered crucial for fibrilization and determines the properties of the silk fiber. We established a technical platform to modify the Fib-H core region systematically using transcription activator-like effector nucle...
Source: Insect Biochemistry and Molecular Biology - September 1, 2023 Category: Biochemistry Authors: Yoko Takasu Nobuto Yamada Katsura Kojima Masatoshi Iga Fumiko Yukuhiro Tetsuya Iizuka Taiyo Yoshioka Source Type: research

Characterisation of geranylgeranyl diphosphate synthase from the sandfly Lutzomyia longipalpis
Insect Biochem Mol Biol. 2023 Aug 22:104001. doi: 10.1016/j.ibmb.2023.104001. Online ahead of print.ABSTRACTLeishmaniasis is a debilitating and often fatal neglected tropical disease. Males from sub-populations of the Leishmania-harbouring sandfly, Lutzomyia longipalpis, produce the diterpene sex and aggregation pheromone, sobralene, for which geranylgeranyl diphosphate (GGPP) is the likely isoprenoid precursor. We have identified a GGPP synthase (lzGGPPS) from L. longipalpis, which was recombinantly expressed in bacteria and purified for functional and kinetic analysis. In vitro enzymatic assays using LC-MS showed that lz...
Source: Insect Biochemistry and Molecular Biology - August 24, 2023 Category: Biochemistry Authors: Charles Ducker Stanley French Monika Pathak Harry Taylor Adam Sainter William Askem Ingrid Dreveny Ant ônio Euzébio Goulart Santana John A Pickett Neil J Oldham Source Type: research

Masculinizer gene controls male sex determination in the codling moth, Cydia pomonella
Insect Biochem Mol Biol. 2023 Aug 1;160:103991. doi: 10.1016/j.ibmb.2023.103991. Online ahead of print.ABSTRACTThe molecular mechanisms of sex determination in moths and butterflies (Lepidoptera) with female heterogamety (WZ/ZZ) are poorly understood, except in the silkworm Bombyx mori. However, the Masculinizer (Masc) gene that controls male development and dosage compensation in B. mori, appears to be conserved in Lepidoptera, as its masculinizing function was recently confirmed in several moth species. In this work, we investigated the role of the Masc gene in sex determination of the codling moth Cydia pomonella (Tortr...
Source: Insect Biochemistry and Molecular Biology - August 3, 2023 Category: Biochemistry Authors: Krist ýna Pospíšilová Arjen E Van't Hof Atsuo Yoshido Renata Kru žíková Sander Visser Magda Zrzav á Kseniya Bobryshava Martina Dal íková Franti šek Marec Source Type: research