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Specialty: Parasitology
Vaccination: Veterinary Vaccinations
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Total 235 results found since Jan 2013.
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Degrade to survive: the intricate world of piroplasmid proteases
Trends Parasitol. 2023 Jun 2:S1471-4922(23)00118-6. doi: 10.1016/j.pt.2023.04.010. Online ahead of print.ABSTRACTPiroplasmids of the genera Babesia, Theileria, and Cytauxzoon are tick-transmitted parasites with a high impact on animals and humans. They have complex life cycles in their definitive arthropod and intermediate vertebrate hosts involving numerous processes, including invasion of, and egress from, host cells, parasite growth, transformation, and migration. Like other parasitic protozoa, piroplasmids are equipped with different types of protease to fulfill many of such essential processes. Blockade of some key pr...
Source: Trends in Parasitology - June 4, 2023 Category: Parasitology Authors: Monica Florin-Christensen Daniel Sojka Sabrina Ganzinelli Pavla Šnebergerová Carlos E Suarez Leonhard Schnittger Source Type: research
Immunization with thiol-binding proteins from Haemonchus contortus adult worms partially protects goats against infection during prepatency
Exp Parasitol. 2023 Mar 23:108512. doi: 10.1016/j.exppara.2023.108512. Online ahead of print.ABSTRACTTo contribute of the knowledge of the immune mechanisms underlying the response to the immunization of goats with thiol-binding proteins fractions (PBS-TSBP) from Haemonchus contortus (H. contortus) adult worms, this study analyzed the degree of protection and the immune responses developed against the parasite after vaccination with this antigenic complex during the time-elapsing between challenge with L3 of the parasite and the development of adult worms, evidenced by the appearance of first faecal eggs (prepatent period ...
Source: Experimental Parasitology - March 25, 2023 Category: Parasitology Authors: J M Molina Y I Hern ández O Ferrer M M Conde-Felipe F Rodr íguez A Ruiz Source Type: research
Functional characterization of differentially expressed proteins coming from unisexual and bisexual infected Schistosoma japonicum female worms
In this study, five highly expressed proteins (S. japonicum large subunit ribosomal protein L7e, S. japonicum glutathione S-transferase class-mu 26 kDa isozyme, S. japonicum UDP-galactose-4-epimerase and two hypothetical proteins SjCAX70849 and SjCAX72486) in 18, 21, 23, and 25-day mature female worms compared to single-sex infected female worms were selected based on our previous proteomic analysis. Quantitative real-time polymerase chain reaction analysis and long-term interference with small interfering RNA were performed to identify the biological functions of these five proteins. The transcriptional profiles suggested...
Source: Experimental Parasitology - March 13, 2023 Category: Parasitology Authors: Haoran Zhong Fanglin Qin Yuqi Ren Xiaochun Li Ling Hou Shaopeng Gu Yamei Jin Source Type: research
Biotechnological Based Recombinant Protein Vaccines Developed Against Toxoplasmosis
Turkiye Parazitol Derg. 2022 Nov 28;46(4):342-357. doi: 10.4274/tpd.galenos.2022.41636.ABSTRACTToxoplasma gondii (T. gondii) that can infect most warm-blooded animals and humans, is an obligate intracellular apicomplexan parasite with a wide host range. About one-third of the world's population is infected with this parasite. While toxoplasmosis progresses asymptomatically in individuals with a strong immune system, it can cause serious clinical manifestations and death in immunocompromised individuals. The parasite is transmitted to humans through the consumption of water and food contaminated with cat feces, as well as r...
Source: Turkish Society for Parasitology - November 29, 2022 Category: Parasitology Authors: Tu ğba Karakavuk Ceren G ül Muhammet Karakavuk Ayt ül Gül Sedef Erkunt Alak H üseyin Can Cemal Ün Mert D öşkaya Adnan Y üksel Gürüz Aysu De ğirmenci Döşkaya Source Type: research
