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Specialty: Molecular Biology
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Total 131414 results found since Jan 2013.
Type II CRISPR-Cas System Nucleases: A Pipeline for Prediction and In Vitro Characterization
Mol Biol (Mosk). 2023 May-Jun;57(3):546-560.ABSTRACTThe use of CRISPR-Cas bacterial adaptive immunity system components for targeted DNA changes has opened broad prospects for programmable genome editing of higher organisms. The most widely used gene editors are based on the Cas9 effectors of the type II CRISPR-Cas systems. In complex with guide RNAs, Cas9 proteins are able to directionally introduce double-stranded breaks into DNA regions that are complementary to guide RNA sequences. Despite the wide range of characterized Cas9s, the search for new Cas9 variants remains an important task, since the available Cas9 editors...
Source: Molekuliarnaia Biologiia - June 16, 2023 Category: Molecular Biology Authors: A A Vasileva S A Aliukas P A Selkova A N Arseniev V E Chernova O S Musharova E I Klimuk M A Khodorkovskii K V Severinov Source Type: research
Molecular methods for diagnosis of monkeypox: A mini-review
CONCLUSION: It is evident there is a pressing need to develop national technologies for rapid diagnosis of emerging diseases for mass testing of the population. It is also extremely important to have national detection kits with greater diagnostic capacity to assist in developing effective public policies in countries affected by this disease.PMID:37461338 | DOI:10.2174/1566524023666230717141920
Source: Current Molecular Medicine - July 18, 2023 Category: Molecular Biology Authors: Rodrigo Michelini de Oliveira Thomasi Thais da Silva Correa Dalise Silva do Carmo D éborah Fernandes Rodrigues Luiz Vinicius da Silva Correa Sandra Rodrigues Xavier L íria Souza Silva Jonatas Oliveira da Silva Michelli Dos Santos Alessandra da Silva Dan Source Type: research
Type II CRISPR-Cas System Nucleases: A Pipeline for Prediction and In Vitro Characterization
Mol Biol (Mosk). 2023 May-Jun;57(3):546-560.ABSTRACTThe use of CRISPR-Cas bacterial adaptive immunity system components for targeted DNA changes has opened broad prospects for programmable genome editing of higher organisms. The most widely used gene editors are based on the Cas9 effectors of the type II CRISPR-Cas systems. In complex with guide RNAs, Cas9 proteins are able to directionally introduce double-stranded breaks into DNA regions that are complementary to guide RNA sequences. Despite the wide range of characterized Cas9s, the search for new Cas9 variants remains an important task, since the available Cas9 editors...
Source: Molekuliarnaia Biologiia - June 16, 2023 Category: Molecular Biology Authors: A A Vasileva S A Aliukas P A Selkova A N Arseniev V E Chernova O S Musharova E I Klimuk M A Khodorkovskii K V Severinov Source Type: research
Accurate Estimation of Context-Dependent False Discovery Rates in Top-Down Proteomics.
We present a new open-source tool, the TDCD_FDR_Calculator, which provides a scalable, context-dependent FDR calculation that can be applied post-search to enhance the quality of results in top-down proteomics from any search engine.
PMID: 30647073 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Proteomics : MCP - January 15, 2019 Category: Molecular Biology Authors: LeDuc RD, Fellers RT, Early BP, Greer JB, Shams DP, Thomas P, Kelleher NL Tags: Mol Cell Proteomics Source Type: research
Distributed learning: a reliable privacy-preserving strategy to change multicenter collaborations using AI
CONCLUSION: Distributed learning resulted in a reliable strategy for model development; indeed, it performed equally to models trained on centralized datasets. Sensitive data can get preserved since they are not shared for model development. Distributed learning constitutes a promising solution for ML-based research and practice since large, diverse datasets are crucial for success.PMID:33847779 | PMC:PMC8041944 | DOI:10.1007/s00259-021-05339-7
Source: Molecular Medicine - April 13, 2021 Category: Molecular Biology Authors: Margarita Kirienko Martina Sollini Gaia Ninatti Daniele Loiacono Edoardo Giacomello Noemi Gozzi Francesco Amigoni Luca Mainardi Pier Luca Lanzi Arturo Chiti Source Type: research
Natural family-free genomic distance
Algorithms Mol Biol. 2021 May 10;16(1):4. doi: 10.1186/s13015-021-00183-8.ABSTRACTBACKGROUND: A classical problem in comparative genomics is to compute the rearrangement distance, that is the minimum number of large-scale rearrangements required to transform a given genome into another given genome. The traditional approaches in this area are family-based, i.e., require the classification of DNA fragments of both genomes into families. Furthermore, the most elementary family-based models, which are able to compute distances in polynomial time, restrict the families to occur at most once in each genome. In contrast, the dis...
Source: Algorithms for Molecular Biology : AMB - May 11, 2021 Category: Molecular Biology Authors: Diego P Rubert F ábio V Martinez Mar ília D V Braga Source Type: research
