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Changes of Gut Microbiota in Maintenance Hemodialysis Patients and Their Impact on Patient's Microinflammation Status
In conclusion, patients with maintenance hemodialysis for CKD commonly exhibit gut microbiota dysbiosis and varying degrees of low-grade inflammation. Compared to healthy individuals, maintenance hemodialysis patients with CKD have lower levels of Bifidobacterium and Lactobacillus and higher levels of Escherichia coli and Enterococcus in their gut. Bifidobacterium, Lactobacillus, Escherichia coli, and Enterococcus all have a certain impact on the low-grade inflammation status of patients with maintenance hemodialysis for CKD.PMID:37715414 | DOI:10.14715/cmb/2023.69.8.15
Source: Mol Biol Cell - September 16, 2023 Category: Molecular Biology Authors: Xiaopan Zhang Yilin Wang Ying Yin Bo Sun Guangren Chen Fengling Chen Source Type: research

Encapsulation of Dicranopteris linearis extract using cellulose microparticles for antiulcer medication
Int J Biol Macromol. 2023 Sep 7:126795. doi: 10.1016/j.ijbiomac.2023.126795. Online ahead of print.ABSTRACTDicranopteris linearis (DL) is a fern in the Gleicheniaceae family, locally known as resam by the Malay community. It has numerous pharmacological benefits, with antiulcer and gastroprotective properties. Peptic ulcer is a chronic and recurring disease that significantly impacts morbidity and mortality, affecting nearly 20 % of the world's population. Despite the effectiveness of peptic ulcer drugs, there is no perfect treatment for the ailment. Encapsulation is an advanced technique that can treat peptic ulcers by in...
Source: International Journal of Biological Macromolecules - September 9, 2023 Category: Biochemistry Authors: Anis Adilah Ahmad Khairul Farihan Kasim Subash C B Gopinath Periasamy Anbu Noor Soffalina Sofian Seng Source Type: research

A simple and high efficiency purification of His-tagged turnip yellow mosaic virus-like particle (TYMV-VLP) by nickel ion affinity precipitation
In this study, we report a non-chromatographic method for VLP purification using C-terminally His-tagged TYMVc (TYMVcHis6) as a protein model. Firstly, the TYMVcHis6 was cloned and expressed in E. coli. Upon clarification of cell lysate, nickel (II) chloride [NiCl2; 15 µM or equivalent to 0.0000194% (w/v)] was added to precipitate TYMVcHis6. Following centrifugation, the pellet was resuspended in buffer containing 1 mM EDTA to chelate Ni2+, which is then removed via dialysis. A total of 50% of TYMVcHis6 was successfully recovered with purity above 0.90. Later, the purified TYMVcHis6 was analysed with sucrose density ultra...
Source: Journal of Virological Methods - July 12, 2023 Category: Virology Authors: Foo Hou Tan Jeck Fei Ng Noorjahan Banu Mohamed Alitheen Azira Muhamad Chean Yeah Yong Khai Wooi Lee Source Type: research

The Epidemiological and Clinical Characteristics of the Largest Outbreak of Enterohemorrhagic < em > Escherichia coli < /em > in Korea
CONCLUSION: Our findings from the response to the largest outbreak will help prepare countermeasures against future EHEC outbreak.PMID:37069811 | PMC:PMC10111046 | DOI:10.3346/jkms.2023.38.e117
Source: J Korean Med Sci - April 18, 2023 Category: General Medicine Authors: Namwoo Heo Jihee Lee Yeonkyung Kim Donghan Lee Seok-Jae Heo Yoon Soo Park Jae-Won Yun Yong Chan Kim Source Type: research

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