Filtered By:
Infectious Disease: Gastroenteritis
Education: Grants
This page shows you your search results in order of date.
Order by Relevance | Date
Total 62 results found since Jan 2013.
The Adjuvant Combination of dmLT and Monophosphoryl Lipid A Activates the Canonical, Nonpyroptotic NLRP3 Inflammasome in Dendritic Cells and Significantly Interacts to Expand Antigen-Specific CD4 T Cells
In this study, we investigated how the nontoxic double mutant Escherichia coli heat-labile toxin R192G/L211A (dmLT), when combined with the TLR4 agonist monophosphoryl lipid A (MPL-A), impacted innate and adaptive immune responses to vaccination in mice. We found that the combination of dmLT and MPL-A induced an expansion of Ag-specific, multifaceted Th1/2/17 CD4 T cells higher than that explained by adding responses to either adjuvant alone. Furthermore, we observed more robust activation of primary mouse bone marrow-derived dendritic cells in the combination adjuvant-treated group via engagement of the canonical NOD-like...
Source: Journal of Immunology - April 6, 2023 Category: Allergy & Immunology Authors: David L Bauer Louay Bachnak Vanessa M Limbert Rebecca M Horowitz Robin L Baudier Shaina J D'Souza Victoria E Immethun Jonathan R Kurtz Samuel B Grant James B McLachlan Source Type: research
Repurposing the Kinase Inhibitor Mavelertinib for Giardiasis Therapy
Antimicrob Agents Chemother. 2022 Jun 15:e0001722. doi: 10.1128/aac.00017-22. Online ahead of print.ABSTRACTA phenotypic screen of the ReFRAME compound library was performed to identify cell-active inhibitors that could be developed as therapeutics for giardiasis. A primary screen against Giardia lamblia GS clone H7 identified 85 cell-active compounds at a hit rate of 0.72%. A cytotoxicity counterscreen against HEK293T cells was carried out to assess hit compound selectivity for further prioritization. Mavelertinib (PF-06747775), a third-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), was ...
Source: Antimicrobial Agents and Chemotherapy - June 15, 2022 Category: Microbiology Authors: Samantha A Michaels Matthew A Hulverson Grant R Whitman Linh T Tran Ryan Choi Erkang Fan Case W McNamara Melissa S Love Kayode K Ojo Source Type: research
The chemotherapeutic drug methotrexate selects for antibiotic resistance
EBioMedicine. 2021 Dec 10;74:103742. doi: 10.1016/j.ebiom.2021.103742. Online ahead of print.ABSTRACTBACKGROUND: Understanding drivers of antibiotic resistance evolution is fundamental for designing optimal treatment strategies and interventions to reduce the spread of antibiotic resistance. Various cytotoxic drugs used in cancer chemotherapy have antibacterial properties, but how bacterial populations are affected by these selective pressures is unknown. Here we test the hypothesis that the widely used cytotoxic drug methotrexate affects the evolution and selection of antibiotic resistance.METHODS: First, we determined me...
Source: Cancer Control - December 13, 2021 Category: Cancer & Oncology Authors: J ónína S Guðmundsdóttir Elizabeth G A Fredheim Catharina I M Koumans Joachim Hegstad Po-Cheng Tang Dan I Andersson Ørjan Samuelsen P ål J Johnsen Source Type: research
Development of a recombinant ELISA for ovine herpesvirus 2, suitable for use in sheep
J Virol Methods. 2021 Oct 12:114329. doi: 10.1016/j.jviromet.2021.114329. Online ahead of print.ABSTRACTThe minor capsid protein of ovine herpesvirus 2, identified as a potential antigen for serological testing, was over-expressed and purified to allow its assessment in ELISA. The corresponding gene sequence (OvHV-2 orf65, Ov65) was modified to incorporate epitope tags and internal restriction enzyme sites in an E. coli codon-optimised version of the gene. This codon-optimised gene was then subject to internal deletions to identify regions of the protein that could be removed while maintaining protein solubility and antige...
Source: Journal of Virological Methods - October 15, 2021 Category: Virology Authors: George C Russell Ann Percival Dawn M Grant Kath Bartley Dylan Turnbull Kevin McLean Julia Lienhard Claudia Bachofen Source Type: research
Maintenance of Metabolic Plasticity despite Relaxed Selection in a Long-Term Evolution Experiment with < em > Escherichia coli < /em >
Am Nat. 2021 Jul;198(1):93-112. doi: 10.1086/714530. Epub 2021 May 21.ABSTRACTAbstractTraits that are unused in a given environment are subject to processes that tend to erode them, leading to reduced fitness in other environments. Although this general tendency is clear, we know much less about why some traits are lost while others are retained and about the roles of mutation and selection in generating different responses. We addressed these issues by examining populations of a facultative anaerobe, Escherichia coli, that have evolved for >30 years in the presence of oxygen, with relaxed selection for anaerobic growth...
Source: The American Naturalist - June 18, 2021 Category: Biology Authors: Nkrumah A Grant Rohan Maddamsetti Richard E Lenski Source Type: research
