Filtered By:
Specialty: Laboratory Medicine

This page shows you your search results in order of relevance. This is page number 2.

Order by Relevance | Date

Total 432 results found since Jan 2013.

Interleukin-18: A Novel Participant in the Occurrence, Development, and Drug Therapy of Obliterative Bronchiolitis Postlung Transplantation
CONCLUSIONS: IL-18 could be a novel molecular involved in the occurrence, development, and drug treatment of OB.PMID:34367377 | PMC:PMC8337162 | DOI:10.1155/2021/5586312
Source: Disease Markers - August 9, 2021 Category: Laboratory Medicine Authors: Ping Shu Wei Zhang Yanfei Zhang Yanfeng Zhao Yuping Li Xiaoqing Zhang Source Type: research

MiR-133b regulates oxidative stress injury of trophoblasts in preeclampsia by mediating the JAK2/STAT3 signaling pathway
AbstractPreeclampsia (PE) is a pregnancy-related syndrome. Aberrant placental microRNAs (miRNAs) expression might associate with PE, including miR-133b. However, its role in the pathogenesis of preeclampsia remains elusive. Therefore, this study explored the role of miR-133b in oxidative stress injury of trophoblasts in preeclampsia (PE) by mediating the JAK2/STAT3 signaling pathway. Placental tissues were collected from PE patients to detect the expression of miR-133b and JAK2/STAT3. Then, in vitro experiments were performed on human extravillous trophoblast-derived HTR-8/SVneo cells, which were divided into Normal, hypox...
Source: Journal of Molecular Histology - October 8, 2021 Category: Laboratory Medicine Source Type: research

RNA interfering targeting human leukocyte antigen-g enhanced immune surveillance mediated by the natural killer cells on hepatocellular carcinoma.
Conclusions Abnormal expression of HLA-G in HCC cell lines plays an important role in protecting against NK cell attack. The significant correlation between HLA-G expression and NK cell lysis implies that the abnormal expression of HLA-G might contribute to the mechanism of escape from host immune surveillance in HCC. PMID: 23694787 [PubMed - in process]
Source: Annals of Clinical and Laboratory Science - May 24, 2013 Category: Laboratory Medicine Authors: Zeng XC, Zhang T, Huang DH, Wang GY, Chen W, Li H, Zhang J, Fang TL, Zhang Q, Chen GH Tags: Ann Clin Lab Sci Source Type: research

Down-regulation of Noggin and miR-138 coordinately promote osteogenesis of mesenchymal stem cells
AbstractMesenchymal stem cells (MSCs) can differentiate to osteocytes under suitable conditions. In recent years, micro-nucleotides have been progressively used to modulate gene expression in cells due to the consideration of safety. Our present study aimed to investigate whether co-delivery of Noggin-siRNA and antimiR-138 enhances the osteogenic effect of MSCs. Using a murine MSC line, C3H/10T1/2 cells, the delivery efficiency of Noggin-siRNA and antimiR-138 into MSCs was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). Cell phenotype and proliferation capacity was assessed by flow cytometry and MT...
Source: Journal of Molecular Histology - November 2, 2017 Category: Laboratory Medicine Source Type: research

Knockdown of Hypoxia-Inducible Factor-1 Alpha Reduces Proliferation, Induces Apoptosis and Attenuates the Aggressive Phenotype of Retinoblastoma WERI-Rb-1 Cells under Hypoxic Conditions.
Conclusion HIF-1α plays a major role in the survival and aggressive phenotype of retinoblastoma cells under hypoxic conditions. Targeting HIF-1α may be a promising therapeutic strategy for human malignant retinoblastoma. PMID: 24795051 [PubMed - in process]
Source: Annals of Clinical and Laboratory Science - May 7, 2014 Category: Laboratory Medicine Authors: Xia T, Cheng H, Zhu Y Tags: Ann Clin Lab Sci Source Type: research

Sirtuin-6 inhibits cardiac fibroblast differentiation into myofibroblasts via inactivation of nuclear factor κB signaling.
Abstract Differentiation of cardiac fibroblasts (CFs) into myofibroblasts represents a key event in cardiac fibrosis that contributes to pathologic cardiac remodeling. However, regulation of this phenotypic transformation remains elusive. Here, we show that sirtuin-6 (SIRT6), a member of the sirtuin family of NAD(+)-dependent histone deacetylase, plays a role in the regulation of myofibroblast differentiation. SIRT6 expression was upregulated under pathologic conditions in angiotensin II (Ang II)-stimulated CFs and in myocardium of rat subject to abdominal aortic constriction surgery. SIRT6 depletion by RNA inter...
Source: Translational Research : the journal of laboratory and clinical medicine - November 13, 2014 Category: Laboratory Medicine Authors: Tian K, Liu Z, Wang J, Xu S, You T, Liu P Tags: Transl Res Source Type: research

Downregulation of angiogenin inhibits the growth and induces apoptosis in human bladder cancer cells through regulating AKT/mTOR signaling pathway
In this study, we constructed ANG siRNA plasmids that transfected into human bladder cancer T24 cells. We demonstrated that knockdown of ANG could inhibit cell proliferation, regulate cell cycle and induce apoptosis. We also found that down-regulation of ANG remarkably reduced the phosphorylation of signaling targets AKT, GSK-3β and mTOR. Furthermore, down-regulation of ANG increased expression of ribonuclease inhibitor, which is a cytoplasmic acidic protein with many functions. Finally, ANG siRNA led to the suppression for tumorigenesis and metastasis in vivo. Taken together, these findings highlight for the first time t...
Source: Journal of Molecular Histology - January 7, 2015 Category: Laboratory Medicine Source Type: research

Anterior gradient 2 is correlated with EGFR mutation in lung adenocarcinoma tissues.
CONCLUSIONS: AGR2 could serve as an adjunctive surrogate protein marker possibly reflecting EGFR gene mutations in lung adenocarcinoma patients. Results from in vitro analysis indicated that AGR2 could be a potential clinical biomarker of EGFR-TKI therapeutic sensitivity in lung adenocarcinoma cells. PMID: 25634032 [PubMed - as supplied by publisher]
Source: International Journal of Biological Markers - January 31, 2015 Category: Laboratory Medicine Tags: Int J Biol Markers Source Type: research

Decreased expression and prognostic role of EHD2 in human breast carcinoma: correlation with E-cadherin
Abstract Decreased expression of epithelial cadherin (E-cadherin) has been noted to associate with aggressiveness and metastasis of breast cancer. The aim of this study was to examine the effect of C-Terminal EH domain-containing protein 2 (EHD2) expression on E-cadherin and related mechanism in the metastasis of breast cancer. Immunohistochemical analysis was performed in 96 human breast carcinoma samples and the data were correlated with clinicopathologic characteristics. Furthermore, Western blot analysis was performed for EHD2 and E-cadherin in breast carcinoma samples and cell lines to evaluate their protein ...
Source: Journal of Molecular Histology - March 12, 2015 Category: Laboratory Medicine Source Type: research

A disintegrin and metalloprotease-10 is correlated with disease activity and mediates monocyte migration and adhesion in rheumatoid arthritis.
We examined the secretion of ADAM-10 in biological fluids from patients with rheumatoid arthritis (RA) and the role it plays in monocyte migration. ADAM-10 levels were measured using enzyme-linked immunosorbent assays and immunofluorescence. To examine the role of ADAM-10 in RA synovial fluids (SFs), we studied THP-1 and monocyte chemotaxis. To determine whether ADAM-10 plays a role in cell proliferation in the RA synovium, we assayed the proliferation of ADAM-10 small interfering RNA (siRNA)-transfected RA fibroblast-like synoviocytes (FLSs). The ADAM-10 level in RA serum was significantly higher than that in normal serum...
Source: Translational Research : the journal of laboratory and clinical medicine - February 28, 2015 Category: Laboratory Medicine Authors: Isozaki T, Ishii S, Nishimi S, Nishimi A, Oguro N, Seki S, Miura Y, Miwa Y, Oh K, Toyoshima Y, Nakamura M, Inagaki K, Kasama T Tags: Transl Res Source Type: research

MRP1 knockdown down-regulates the deposition of collagen and leads to a reduced hypertrophic scar fibrosis
Abstract Multidrug resistance-associated protein 1 (MRP1) belongs to ATP-binding cassette transporters family. The overexpression of MRP1 is predominantly related with the failure of chemo-radiotherapy in various tumors. However, its possible role in hypertrophic scar (HS) is hardly investigated. Here we showed that the mRNA level and protein expression of MRP1 were higher in HS and HS derived fibroblasts (HSFs) than that in normal skin (NS) and NS derived fibroblasts (NSFs). Immunohistochemistry and immunofluorescence showed that the percentage of positive cells was higher in HS and HSFs. Meanwhile, the co-local...
Source: Journal of Molecular Histology - June 20, 2015 Category: Laboratory Medicine Source Type: research

Wnt pathway in the Dupuytren disease connecting profibrotic signals.
In conclusion, we have shown extensive dysregulation of Wnt signaling in affected tissue from the Dupuytren disease patients. Components of both the canonical and the noncanonical pathways are upregulated, whereas endogenous antagonists are downregulated, possibly via interaction with other profibrotic pathways. PMID: 26470681 [PubMed - as supplied by publisher]
Source: Translational Research : the journal of laboratory and clinical medicine - September 25, 2015 Category: Laboratory Medicine Authors: van Beuge MM, Ten Dam EP, Werker PM, Bank RA Tags: Transl Res Source Type: research

Targeting hypoxia-mediated mucin 2 production as a therapeutic strategy for mucinous tumors.
Abstract Excessive accumulation of mucin 2 (MUC2; a gel-forming secreted mucin) protein in the peritoneal cavity is the major cause of morbidity and mortality in pseudomyxoma peritonei (PMP). Hypoxia/hypoxia-inducible factor-1α (HIF-1α) has been shown to regulate the expression of similar mucins (eg, MUC5AC). We hypothesized that hypoxia/HIF-1α drives MUC2 expression in PMP and is therefore a novel target to reduce mucinous tumor growth. The regulation of MUC2 by 2% hypoxia/HIF-1α was evaluated in MUC2-secreting LS174T cells. The effect of BAY 87-2243, an inhibitor of HIF-1α, on MUC2 expression and mucinous t...
Source: Translational Research : the journal of laboratory and clinical medicine - October 31, 2015 Category: Laboratory Medicine Authors: Dilly AK, Lee YJ, Zeh HJ, Guo ZS, Bartlett DL, Choudry HA Tags: Transl Res Source Type: research