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Vaccination: Vaccines
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Total 344 results found since Jan 2013.
An adventitious agent-free clonal cell line that is highly susceptible to foot -and-mouth disease virus
In this study, we developed a three-prong methodology to completely remove BVDV from LFBKαVβ6 cells. Combined treatment with siRNA against BVDV NS5A, porcine interferon alpha and ribavirin resulted in the elimination of BVDV, as determined by immunohistochemistry analysis, quantitative RT-PCR and RNA sequencing. Importantly, elimination of BVDV from LFBKαVβ6 did not affect FMDV growth and plaque phenotype from different serotypes isolated and propagated in the clean cell line, newly named MGPK αVβ6-C5. Additionally, isolation of FMDV from field oro-pharyngeal samples, was successful at the same sensitivity as in BVDV...
Source: Biologicals : Journal of the International Association of Biological Standardization - June 7, 2021 Category: Biotechnology Authors: M LaRocco Z Ahmed M Rodriguez-Calzada P A Azzinaro R Barrette P Krug L L Rodriguez T de Los Santos G N Medina Source Type: research
Viruses, Vol. 13, Pages 1533: Inhibitors of Venezuelan Equine Encephalitis Virus Identified Based on Host Interaction Partners of Viral Non-Structural Protein 3
In this study, we utilized an overexpression construct encoding HA-tagged nsP3 to identify host proteins that interact with VEEV nsP3 by mass spectrometry. Bioinformatic analyses of the putative interactors identified 42 small molecules with the potential to inhibit the host interaction targets, and thus potentially inhibit VEEV. Three inhibitors, tomatidine, citalopram HBr, and Z-VEID-FMK, reduced replication of both the TC-83 strain and the Trinidad donkey (TrD) strain of VEEV by at least 10-fold in astrocytoma, astroglial, and microglial cells. Further, these inhibitors reduced replication of the related New World (NW) ...
Source: Viruses - August 3, 2021 Category: Virology Authors: Allison Bakovic Nishank Bhalla Farhang Alem Catherine Campbell Weidong Zhou Aarthi Narayanan Tags: Article Source Type: research
Shrimp Parvovirus Circular DNA Fragments Arise From Both Endogenous Viral Elements and the Infecting Virus
Some insects use endogenous reverse transcriptase (RT) to make variable viral copy DNA (vcDNA) fragments from viral RNA in linear (lvcDNA) and circular (cvcDNA) forms. The latter form is easy to extract selectively. The vcDNA produces small interfering RNA (siRNA) variants that inhibit viral replication via the RNA interference (RNAi) pathway. The vcDNA is also autonomously inserted into the host genome as endogenous viral elements (EVE) that can also result in RNAi. We hypothesized that similar mechanisms occurred in shrimp. We used the insect methods to extract circular viral copy DNA (cvcDNA) from the giant tiger shrimp...
Source: Frontiers in Immunology - September 28, 2021 Category: Allergy & Immunology Source Type: research
Developing Biodegradable Lipid Nanoparticles for Intracellular mRNA Delivery and Genome Editing
Acc Chem Res. 2021 Oct 20. doi: 10.1021/acs.accounts.1c00500. Online ahead of print.ABSTRACTConspectusSince the U.S. Food and Drug Administration (FDA) granted emergency use authorization for two mRNA vaccines against SARS-CoV-2, mRNA-based technology has attracted broad attention from the scientific community to investors. When delivered intracellularly, mRNA has the ability to produce various therapeutic proteins, enabling the treatment of a variety of illnesses, including but not limited to infectious diseases, cancers, and genetic diseases. Accordingly, mRNA holds significant therapeutic potential and provides a promis...
Source: Cell Research - October 20, 2021 Category: Cytology Authors: Min Qiu Yamin Li Hanan Bloomer Qiaobing Xu Source Type: research
MMV-db: vaccinomics and RNA-based therapeutics database for infectious hemorrhagic fever-causing mammarenaviruses
AbstractThe recent viral outbreaks and the current pandemic situation urges us to timely address any emerging viral infections by designing therapeutic strategies. Multi-omics and therapeutic data are of great interest to develop early remedial interventions. This work provides a therapeutic data platform (Mammarenavirus (MMV)-db) for pathogenic mammarenaviruses with potential catastrophic effects on human health around the world. The database integrates vaccinomics and RNA-based therapeutics data for seven human pathogenic MMVs associated with severe viral hemorrhagic fever and lethality in humans. Protein-specific cytoto...
Source: Database : The Journal of Biological Databases and Curation - October 22, 2021 Category: Databases & Libraries Source Type: research
Potential inhibitors and plant based technology: An alternative approach to treat corona virus
Bioorg Chem. 2021 Nov 1;117:105460. doi: 10.1016/j.bioorg.2021.105460. Online ahead of print.ABSTRACTThe current pneumonia outbreak, which began in early December 2019 near Wuhan City, Hubei Province, China, is caused by a novel corona virus (CoV) known as '2019-nCoV' or '2019 novel corona virus or COVID-19' by the World Health Organization (WHO). Vaccines are available to prevent corona virus contagious infection or to reduce the viral load in body but virus is continuously mutating itself to infect people at severity. In this critical scenario this review provide a compiled study for techniques and tools that can be used...
Source: Bioorganic Chemistry - November 11, 2021 Category: Chemistry Authors: Sachin Sharma Chetana Suvalka None Bharti Manoj Joshi Ayush Bahuguna Source Type: research
