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Specialty: Molecular Biology
Nutrition: Fish
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Total 526 results found since Jan 2013.
Effect of Different Plant Extracts on Microbial Population in The Frozen African Catfish (Clarias gariepinus) Semen
This study sought to assess the potential of various plant extracts to function as natural antimicrobial agents in the frozen semen of African catfish (Clarias gariepinus). Diluted sperm was packaged in 0.25ml straws and left for 10min equilibration at 4°C. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into the liquid nitrogen (-196°C) and then thawed in a water bath at 35°C for 20s. Sperm samples were put into sterile 1.5 ml tubes immediately after thawing and the microbial count was detected with classical microbiological culture method. In the results of microbiologi...
Source: Mol Biol Cell - September 16, 2023 Category: Molecular Biology Authors: Faik Sertel Secer Ilker Yavas Zafer Cantekin Yusuf Bozkurt Tugba Korkmaz Yavas Alexander Atanasoff Source Type: research
Quantitative Live Imaging of Zebrafish Scale Regeneration: From Adult Fish to Signaling Patterns and Tissue Flows
Methods Mol Biol. 2024;2707:185-204. doi: 10.1007/978-1-0716-3401-1_12.ABSTRACTIn regeneration, a damaged body part grows back to its original form. Understanding the mechanisms and physical principles underlying this process has been limited by the difficulties of visualizing cell signals and behaviors in regeneration. Zebrafish scales are emerging as a model system to investigate morphogenesis during vertebrate regeneration using quantitative live imaging. Scales are millimeter-sized dermal bone disks forming a skeletal armor on the body of the fish. The scale bone is deposited by an adjacent monolayer of osteoblasts tha...
Source: Mol Biol Cell - September 5, 2023 Category: Molecular Biology Authors: Alessandro De Simone Source Type: research
Microbial oil, alone or paired with β-glucans, can control hypercholesterolemia in a zebrafish model
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Aug 30:159383. doi: 10.1016/j.bbalip.2023.159383. Online ahead of print.ABSTRACTDyslipidemia is often associated with unhealthy dietary habits, and many mammalian studies have explored the mode of action of certain bioactive compounds such as β-glucans and n-3 PUFAs to understand their potential to normalize the lipid metabolism. There are only a few investigations of their combined effect on hypercholesterolemia by adopting omics approaches. Zebrafish (Danio rerio) was used as a model organism to reveal the efficacy of Schizochytrium oil and β-glucans (from Euglena gracili...
Source: Mol Biol Cell - September 1, 2023 Category: Molecular Biology Authors: Adnan H Gora Saima Rehman Jorge Dias Jorge M O Fernandes P ål A Olsvik Mette S ørensen Viswanath Kiron Source Type: research
Isolation of Tissue Macrophages in Adult Zebrafish
Methods Mol Biol. 2024;2713:81-98. doi: 10.1007/978-1-0716-3437-0_5.ABSTRACTTissue macrophages are essential components of the immune system that also play key roles in vertebrate development and homeostasis, including in zebrafish, which has gained popularity over the years as a translational model for human disease. Commonly, zebrafish macrophages are identified based on expression of fluorescent transgenic reporters, allowing for real-time imaging in living animals. Several of these lines have also proven instrumental to isolate pure populations of macrophages in the developing embryo and larvae using fluorescence-activ...
Source: Mol Biol Cell - August 28, 2023 Category: Molecular Biology Authors: Mireia Rovira Jennifer Pozo Magali Miserocchi Val érie Wittamer Source Type: research
Characteristic of the gene candidate SecARS encoding alkylresorcinol synthase in Secale
CONCLUSIONS: The conducted analyses contributed to a better understanding of the processes related to ARs synthesis. Although the research concerned the rye model, the knowledge gained may help in understanding the genetic basis of ARs biosynthesis in other species of the Poaceae family as well.PMID:37615923 | DOI:10.1007/s11033-023-08684-y
Source: Molecular Biology Reports - August 24, 2023 Category: Molecular Biology Authors: Ma łgorzata Targońska-Karasek Micha ł Kwiatek Jolanta Groszyk Jakub Walczewski Mariusz Kowalczyk Sylwia Pawelec Maja Boczkowska Anna Ruci ńska Source Type: research
CircZSWIM4 facilitates tumor development in lung adenocarcinoma by targeting miR-370-3p and miR-873-5p to regulate the axis of FOXM1/ β-catenin
Cell Mol Biol (Noisy-le-grand). 2023 Jun 30;69(6):132-140. doi: 10.14715/cmb/2023.69.6.20.ABSTRACTCircular RNA (circRNA) is a kind of RNA generated by a covalently closed loop and possesses sophisticated capacities of gene regulation in tumorigenesis and development. However, the role of circZSWIM4 on lung adenocarcinoma (LUAD) remains largely unclear. In the present study, we used reverse transcription-qPCR (RT-qPCR) to examine whether circZSWIM4 was significantly overexpressed in LUAD cells. The impacts of circZSWIM4 on the properties of proliferation, apoptosis and migration were assessed by loss-of and gain-of-function...
Source: Mol Biol Cell - August 22, 2023 Category: Molecular Biology Authors: Zhigang Fan Hongyan Wang Source Type: research
Induced swimming modified the antioxidant status of gilthead seabream (Sparus aurata)
Comp Biochem Physiol B Biochem Mol Biol. 2023 Aug 19:110893. doi: 10.1016/j.cbpb.2023.110893. Online ahead of print.ABSTRACTSwimming has relevant physiological changes in farmed fish, although the potential link between swimming and oxidative stress remains poorly studied. We investigated the effects of different medium-term moderate swimming conditions for 6 h on the antioxidant status of gilthead seabream (Sparus aurata), analyzing the activity of enzymes related to oxidative stress in the liver and skeletal red and white muscle. Forty fish were induced to swim individually with the following conditions: steady low (SL, ...
Source: Mol Biol Cell - August 21, 2023 Category: Molecular Biology Authors: Carlos Esp írito-Santo Carmen Alburquerque Francisco A Guardiola Rodrigo O A Oz ório Leonardo J Magnoni Source Type: research
Transcription modulates chromatin dynamics and locus configuration sampling
Nat Struct Mol Biol. 2023 Aug 3. doi: 10.1038/s41594-023-01059-8. Online ahead of print.ABSTRACTIn living cells, the 3D structure of gene loci is dynamic, but this is not revealed by 3C and FISH experiments in fixed samples, leaving a notable gap in our understanding. To overcome these limitations, we applied the highly predictive heteromorphic polymer (HiP-HoP) model to determine chromatin fiber mobility at the Pax6 locus in three mouse cell lines with different transcription states. While transcriptional activity minimally affects movement of 40-kbp regions, we observed that motion of smaller 1-kbp regions depends strong...
Source: Mol Biol Cell - August 3, 2023 Category: Molecular Biology Authors: Giada Forte Adam Buckle Shelagh Boyle Davide Marenduzzo Nick Gilbert Chris A Brackley Source Type: research
The Integral Role of Diets Including Natural Products to Manage Rheumatoid Arthritis: A Narrative Review
Curr Issues Mol Biol. 2023 Jun 28;45(7):5373-5388. doi: 10.3390/cimb45070341.ABSTRACTGenetic and environmental factors including lifestyle are thought to play a key role in the pathophysiology of rheumatoid arthritis (RA). There is evidence that diet can enhance the inflammatory response in genetically predisposed individuals. On the other hand, certain types of diets can alleviate RA symptoms due to their anti-inflammatory and antioxidant activities. Also, natural compounds with potential effectiveness in RA management belong to different chemical classes such as flavonoids, polyphenols, carotenoids, and alkaloids with th...
Source: Current Issues in Molecular Biology - July 28, 2023 Category: Molecular Biology Authors: Ayse Gunes-Bayir Beyza Mendes Agnes Dadak Source Type: research
