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Specialty: Biology
Source: Life Sciences
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Total 146 results found since Jan 2013.
Elevated expression of hypoxia-inducible factor-2alpha regulated catabolic factors during intervertebral disc degeneration
Publication date: Available online 26 June 2019Source: Life SciencesAuthor(s): Yuelong Huang, Ying Wang, Chengai Wu, Wei TianAbstractHeadings aimsThe present study determined whether nucleus pulposus (NP) cells express hypoxia-inducible factor-2alpha (HIF-2α) and assessed its role in regulating the expression of catabolic factors during intervertebral disc degeneration.Materials and methodsHuman degenerated NP tissues were acquired to examine the HIF-2α expression levels using immunohistochemistry, western blotting, and Real-time PCR. Human NP cells were cultivated under normoxic or hypoxic conditions, and the HIF-2α ex...
Source: Life Sciences - June 26, 2019 Category: Biology Source Type: research
Hispidulin exhibits neuroprotective activities against cerebral ischemia reperfusion injury through suppressing NLRP3-mediated pyroptosis
ConclusionHispidulin is a neuroprotective agent with clinical potential against IR-induced neurological injury.
Source: Life Sciences - June 25, 2019 Category: Biology Source Type: research
Role of PGRMC1 in cell physiology of cervical cancer
In this study, we performed 2D-DIGE and MALDI-TOF/TOF MS to analyze differentially expressed proteins between HeLa and invasive HeLa-I5 cells..Key findingsAccording to our proteomics data, 68 differentially expressed proteins between the HeLa and HeLa-I5 cells were identified. One of these differentially expressed proteins, Progesterone receptor membrane component 1 (PGRMC1), was selected as a candidate for further studies. To correlate the role of PGRMC1 with cellular migration and cancer progression, small interfering RNA (siRNA) was used to knockdown the expression of PGRMC1. Similar function of PGRMC1 was also observed...
Source: Life Sciences - June 18, 2019 Category: Biology Source Type: research
P120-catenin regulates pulmonary fibrosis and TGF-β induced lung fibroblast differentiation
In this study, we investigated the expression and role of P120 in pulmonary fibrosis and transforming growth factor beta (TGF-β) mediated lung fibroblast-to-myofibroblast differentiation (fibroblast differentiation). Our data indicated that P120 expression increased in lung fibrotic foci and primary lung fibroblasts isolated from bleomycin- (BLM) challenged mice, compared to controls. In vitro, TGF-β induced P120 expression in human lung fibroblasts, and siRNA-mediated SMAD3 depletion inhibited TGF-β stimulated P120 expression. Blocking nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) pathway throu...
Source: Life Sciences - May 22, 2019 Category: Biology Source Type: research
Induction of let-7e gene expression attenuates oncogenic phenotype in HCT-116 colorectal cancer cells through targeting of DCLK1 regulation
Publication date: Available online 8 May 2019Source: Life SciencesAuthor(s): Alisa Khodadadi Kohlan, Massoud Saidijam, Razieh Amini, Pouria Samadi, Rezvan NajafiAbstractAimsMicroRNAs (miRNAs) are small noncoding RNAs that negatively control gene expression at the translational level. There are compelling evidences indicating that the expression of let-7e is downregulated in various cancers, however, the role of let-7e in colorectal cancer (CRC) and its mechanism has been remained unknown. Here, we investigated the potential role of let-7e in regulating CRC cells phenotypes.Main methodsLet-7e and DCLK1 siRNA were transfecte...
Source: Life Sciences - May 8, 2019 Category: Biology Source Type: research
MicroRNA-320c inhibits development of osteoarthritis through downregulation of canonical Wnt signaling pathway
Publication date: Available online 8 May 2019Source: Life SciencesAuthor(s): Shu Hu, Guping Mao, Ziji Zhang, Peihui Wu, Xingzhao Wen, Weiming Liao, Zhiqi ZhangAbstractAimsOsteoarthritis (OA) is a leading cause of deformity in aging people. Emerging evidence suggests that microRNAs and Wnt signaling pathway are associated with its pathogenesis. We aimed to determine whether microRNA-320c inhibits the development of osteoarthritis by suppressing Wnt signaling pathway.Materials and methodsMiR-320c and β-catenin expression was assessed in human adipose derived stem cells (hADSCs) model of chondrogenesis and in normal and OA p...
Source: Life Sciences - May 8, 2019 Category: Biology Source Type: research
Downregulation of endothelin A receptor (ETaR) ameliorates renal ischemia reperfusion injury by increasing nitric oxide production
Publication date: Available online 7 May 2019Source: Life SciencesAuthor(s): Long Li, Xia Wang, Long Zheng, Jiawei Li, Ming Xu, Ruiming Rong, Tongyu Zhu, Yichen JiaAbstractAimsTo investigate the protective effects of downregulating ETaR expression on renal ischemia reperfusion injury (IRI).Main methodsThe renal IRI model was generated by clamping the left renal artery for 60 min followed by nephrectomy of the right kidney. ETaR siRNA were perfused through the renal artery during ischemia. HE staining was performed to assess histological injury. PCR was performed to determine the expression of NF-κb, TNF-α, IFN-γ, IL-6...
Source: Life Sciences - May 8, 2019 Category: Biology Source Type: research
CC chemokine receptor 2 functions in osteoblastic transformation of valvular interstitial cells
Publication date: Available online 26 April 2019Source: Life SciencesAuthor(s): Enyi Zhu, Zihao Liu, Wanbing He, Bingqing Deng, Xiaorong Shu, Zhijian He, Xiaoying Wu, Xiao Ke, Ruqiong NieAbstractAimsCalcific aortic valve disease (CAVD) emerges as a challenging clinical issue, which is associated with high cardiovascular mortality. It has been demonstrated that osteoblastic transformation of AVICs is a key mechanism of CAVD and C-C motif chemokine receptors (CCRs) may favor this process. Thus, we aimed to investigate whether CCRs were involved in osteoblastic transformation of AVICs during the development CAVD.Main methodsW...
Source: Life Sciences - April 28, 2019 Category: Biology Source Type: research
Synergistic effects of 7-O-geranylquercetin and siRNAs on the treatment of human breast cancer
Publication date: Available online 19 April 2019Source: Life SciencesAuthor(s): Jiaxin Zuo, Yameng Jiang, Enxia Zhang, Yuling Chen, Ze Liang, Jie Zhu, Yinan Zhao, Hong Xu, Guoliang Liu, Jiasi Liu, Wei Wang, Shubiao Zhang, Yuhong ZhenAbstractAimsTo investigate the antitumor effect of 7-O-geranylquercetin (GQ) combining with survivin siRNA (siSuvi) or IL-10 siRNA (siIL-10) to breast cancer.Main methodsXenograft tumor model was established by subcutaneously inoculating human breast cancer MCF-7 cells in BALB/c nude mice. Transfection efficiency of siRNA mediated by cationic liposome CDO14 in MCF-7 cells and tumor bearing mice...
Source: Life Sciences - April 19, 2019 Category: Biology Source Type: research
Knockdown of Delta-like 3 restricts lipopolysaccharide-induced inflammation, migration and invasion of A2058 melanoma cells via blocking Twist1-mediated epithelial-mesenchymal transition
Publication date: Available online 11 April 2019Source: Life SciencesAuthor(s): Xiaojie Ding, Fuyao Li, Li ZhangAbstractAimsTo investigate the effects and mechanisms of DLL3 in inflammation-mediated A2058 melanoma cell invasion and metastasis.Materials and methodsMelanoma A2058 cells was stimulated with lipopolysaccharide (LPS), with or without transfection of DLL3 siRNA, or DLL3 overexpression vector, or Twist1 siRNA. Cell migration and invasion were detected by wound healing and transwell invasion assay. The production of inflammatory factors TNF-α and IL-6 was measured by ELISA. The expression of Notch signaling-relate...
Source: Life Sciences - April 13, 2019 Category: Biology Source Type: research
HIV-1 Nef-GCC185 interaction regulates assembly of cellular protein complexes at TGN targeting MHC-I downregulation
Publication date: Available online 3 April 2019Source: Life SciencesAuthor(s): Sushila Kumari, Manjeet Kumar, Richa Verma, Jimut Kanti Ghosh, Raj Kamal TripathiAbstractAimHIV-1 Nef downregulates surface MHC-I to protect the infected cells from CTLs-mediated killing. Although MHC-I downregulation has been extensively studied, the Nef-dependent assembly of the multi-protein complex and subsequent pathways activation has not yet been well explored. The present study is aimed for the identification of Nef-mediated sequential recruitment of cellular proteins that constitute the functional multi-protein complex, required for the...
Source: Life Sciences - April 5, 2019 Category: Biology Source Type: research
FABP4 silencing ameliorates hypoxia reoxygenation injury through the attenuation of endoplasmic reticulum stress-mediated apoptosis by activating PI3K/Akt pathway
Publication date: Available online 21 March 2019Source: Life SciencesAuthor(s): Tianming Deng, Yanhong Wang, Chongchong Wang, Hua YanAbstractEndoplasmic reticulum (ER) stress and subsequent apoptosis play a vital role in myocardial ischemia reperfusion (IR) injury. Fatty acid binding protein 4 (FABP4) may induce ER stress. The aim of this study was to investigate the mechanism and effect of FABP4 on IR injury in vitro. Rat H9c2 cells were exposed to hypoxia reoxygenation (HR) to create an IR model in vitro. FABP4 was overexpressed in HR-injured H9c2 cells. Transfection with FABP4 siRNA increased cell viability and decrease...
Source: Life Sciences - March 21, 2019 Category: Biology Source Type: research
Z-Guggulsterone attenuates glucocorticoid-induced osteoporosis through activation of Nrf2/HO-1 signaling
Publication date: Available online 21 March 2019Source: Life SciencesAuthor(s): Yier Xu, Jian Guan, Jianyu Xu, Shuilin Chen, Guicai SunAbstractAimsThe present study aims to investigate the protective effect and underlying mechanism of Z-Guggulsterone (Z-GS), an active component from myrrh, on glucocorticoid-induced osteoporosis (GIO).Main methodsGIO rats were used to simulate osteoporosis in vivo while MC3T3-E1 cells were induced to osteoblast differentiation and treated with dexamethasone to simulate osteoporosis in vitro. The rats and cells were treated with Z-GS according to the protocol. The bone mineral density, biome...
Source: Life Sciences - March 21, 2019 Category: Biology Source Type: research
Cytoprotective effects of euxanthone against ox-LDL-induced endothelial cell injury is mediated via Nrf2
Publication date: Available online 16 March 2019Source: Life SciencesAuthor(s): Shengnan Li, Yong Sun, Zhiwu Han, Xiaocui Bu, Weijie Yu, Junyu WangAbstractAimAtherosclerosis (AS) is a chronic condition of the arterial vessels and a risk factor for myocardial infarction and stroke. Euxanthone is a xanthone compound extracted from Polygala caudata, and shows vasodilatory action. The aim of this study was to determine the potential pharmacological effects of euxanthone against oxidized low-density lipoprotein (ox-LDL)-induced endothelial cell injury.Material and methodsHuman umbilical vein endothelial cells (HUVECs) were expo...
Source: Life Sciences - March 17, 2019 Category: Biology Source Type: research
MiR-451 antagonist protects against cardiac fibrosis in streptozotocin-induced diabetic mouse heart
Publication date: Available online 11 March 2019Source: Life SciencesAuthor(s): Cui Liang, Lu Gao, Yuan Liu, Yuzhou Liu, Rui Yao, Yapeng Li, Lili Xiao, Leiming Wu, Binbin Du, Zhen Huang, Yanzhou ZhangAbstractAimsMicroRNAs (miRNAs or miRs) are a large class of small noncoding RNAs. The present study aims to evaluate the effect of miR-451 on cardiac remodeling in diabetic cardiomyopathy.Main methodsMice were injected with streptozotocin (STZ) to induce diabetes. Twelve weeks after final STZ injection, mice were subjected to myocardial injection of adenovirus (Ad)-shmiR-451 to knock down miR-451. Mouse heart endothelial cells...
Source: Life Sciences - March 12, 2019 Category: Biology Source Type: research
