Filtered By:
Source: Biology of Reproduction

This page shows you your search results in order of relevance. This is page number 3.

Order by Relevance | Date

Total 100 results found since Jan 2013.

Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.
We report here that Cnot7, Cnot6l, and Pan2, key components of deadenylation machinery are also dormant maternal mRNAs that are recruited during oocyte maturation. Inhibiting the maturation-associated increase in CNOT7 (or CNOT6L) using an siRNA approach inhibits mRNA deadenylation, whereas inhibiting the increase in PAN2 has little effect. Reciprocally, expressing CNOT7 (or CNOT6L) in oocytes prevented from resuming meiosis initiates deadenylation of mRNAs. These effects on deadenylation are also observed when the total amount of poly(A) is quantified. Last, inhibiting the increase in CNOT7 protein results in an ~70% decr...
Source: Biology of Reproduction - July 1, 2015 Category: Reproduction Medicine Authors: Ma J, Fukuda Y, Schultz RM Tags: Biol Reprod Source Type: research

The Role of Endometrial Selectins and Their Ligands on Bovine Conceptus Attachment to the Uterine Epithelium During Peri-Implantation Period.
The objectives of this study were to determine if selectins and their ligands were expressed in the bovine conceptus and/or uterus during the peri-attachment period, and to study whether selectins were associated with conceptus attachment to the uterine epithelium. Through the RNA-seq analysis of bovine conceptuses on Days 17, 20, and 22 (Day 0 = day of estrus), only the L-selectin (SELL) ligand, podocalyxin (PODXL) and P-selectin (SELP) ligand, SELPLG were found. Quantitative PCR analysis confirmed the presence of PODXL and SELPLG in these conceptuses and revealed that SELL, mRNA and protein, detected in the uterine epith...
Source: Biology of Reproduction - July 1, 2015 Category: Reproduction Medicine Authors: Bai R, Kusama K, Sakurai T, Bai H, Wang C, Zhang J, Kuse M, Ideta A, Aoyagi Y, Okuda K, Imakawa K Tags: Biol Reprod Source Type: research

Progestin and adipoQ Receptor 7, Progesterone Membrane Receptor Component 1 (PGRMC1) and PGRMC2 and Their Role in Regulating Progesterone's Ability to Suppress Human Granulosa/Luteal Cells from Entering into the Cell Cycle.
Abstract The present studies were designed to determine the role of Progesterone Receptor Membrane Component 1 (PGRMC1), PGRMC2, Progestin and AdipoQ Receptor 7 (PAQR7) and Progesterone Receptor (PGR) in mediating the anti-mitotic action of progesterone (P4) in human granulosa/luteal cells. For these studies granulosa/luteal cells of ten women undergoing controlled ovarian hyperstimulation were isolated, maintained in culture, and depleted of PGRMC1, PGRMC2, PAQR7 or PGR by siRNA treatment. The rate of entry into the cell cycle was assessed using the FUCCI cell cycle sensor to determine the percentage of cells in ...
Source: Biology of Reproduction - July 22, 2015 Category: Reproduction Medicine Authors: Sueldo C, Liu X, Peluso JJ Tags: Biol Reprod Source Type: research

Evidence Supporting a Role for SMAD2/3 in Bovine Early Embryonic Development: Potential Implications for Embryotropic Actions of Follistatin.
In conclusion, the present work provides evidence supporting a functional role of SMAD2/3 in bovine early embryogenesis and that specific stimulatory actions of follistatin are not observed in the absence of SMAD2/3 signaling. PMID: 26289443 [PubMed - as supplied by publisher]
Source: Biology of Reproduction - August 19, 2015 Category: Reproduction Medicine Authors: Zhang K, Rajput SK, Lee KB, Wang D, Huang J, Folger JK, Knott JG, Zhang J, Smith GW Tags: Biol Reprod Source Type: research

Human Placenta-Derived Multipotent Stromal Cells Involved in Placental Angiogenesis via the PDGF-BB and STAT3 Pathways.
In conclusion, endothelial cell-hPMSC interactions occur during vessel development of placenta. Endothelial cell-derived PDGF-BB recruits hPMSCs involved in vascular development via PDGFR-beta/STAT3 activation. PMID: 26353894 [PubMed - as supplied by publisher]
Source: Biology of Reproduction - September 9, 2015 Category: Reproduction Medicine Authors: Chen CY, Liu SH, Chen CY, Chen PC, Chen CP Tags: Biol Reprod Source Type: research

Deletion of Arid1a in Reproductive Tract Mesenchymal Cells Reduces Fertility in Female Mice.
Abstract Women with endometriosis can suffer from decreased fecundity or complete infertility via abnormal oocyte function or impaired placental-uterine interactions required for normal pregnancy establishment and maintenance. Although AT-rich interactive domain 1A (SWI-like) (ARID1A) is a putative tumor suppressor in human endometrial cancers and endometriosis-associated ovarian cancers, little is known about its role in normal uterine function. To study the potential function of ARID1A in the female reproductive tract, we generated mice with a conditional knockout of Arid1a using anti-Müllerian hormone receptor...
Source: Biology of Reproduction - March 9, 2016 Category: Reproduction Medicine Authors: Wang X, Khatri S, Broaddus R, Wang Z, Hawkins SM Tags: Biol Reprod Source Type: research

Adiponectin Inhibits Nutrient Transporters and Promotes Apoptosis in Human Villous Cytotrophoblasts: Involvement in the Control of Fetal Growth.
Abstract The placenta exchanges nutrients between the mother and the fetus, and requires a constant abundant energy supply. Adiponectin (a cytokine produced primarily by adipose tissue) controls glucose and lipid homeostasis. It is well known that maternal serum adiponectin levels are inversely related to birth weight suggesting that adiponectin has a negative effect on fetal growth. This effect appears to be related to the control of nutrient transporters in human placenta. However, the underlying molecular mechanisms have not yet been characterized. In the present work, we studied adiponectin's direct effect on ...
Source: Biology of Reproduction - March 29, 2016 Category: Reproduction Medicine Authors: Duval F, Dos Santos E, Poidatz D, Sérazin V, Gronier H, Vialard F, Dieudonné MN Tags: Biol Reprod Source Type: research

MicroRNA-183~96~182 Cluster Regulate Bovine Granulosa Cell Proliferation and Cell Cycle Transition by Coordinately Targeting FOXO1.
Abstract Large-scale expression profiling of microRNAs (miRNAs) in bovine granulosa cells from dominant and subordinate follicles on Day 19 of the estrous cycle revealed enriched microRNA-183~96~182 (miR-183~96~182) cluster miRNAs in preovulatory dominant follicles that coordinately regulate the forkhead box protein O1 (FOXO1) gene. However, little is known about the role of this cluster in bovine granulosa cell function. We employed an in vitro granulosa cell culture model to investigate this role. Granulosa cells aspirated from small growing follicles (3-5 mm in diameter) were cultured in DMEM/F-12 medium supple...
Source: Biology of Reproduction - April 26, 2016 Category: Reproduction Medicine Authors: Gebremedhn S, Salilew-Wondim D, Hoelker M, Rings F, Neuhoff C, Tholen E, Schellander K, Tesfaye D Tags: Biol Reprod Source Type: research

High Estradiol Concentrations Induce Heat Shock Protein 70 Expression and Suppress Nuclear Factor Kappa B Activation in Human Endometrial Epithelial Cells.
In conclusion, high E2 concentrations enhance HSF-1 and Hsp70 expression in EECs. The induced Hsp70 forms a complex with IKK-gamma and inhibits pI kappa B-alpha, which consequently suppresses NF-kappa B activation. PMID: 27605343 [PubMed - as supplied by publisher]
Source: Biology of Reproduction - September 6, 2016 Category: Reproduction Medicine Authors: Chen CD, Chen SU, Chou CH, Chen MJ, Wen WF, Wu SY, Yang YS, Yang JH Tags: Biol Reprod Source Type: research

Iron suppresses ovarian granulosa cell proliferation and arrests cell cycle through regulating p38 MAPK/p53/p21 pathway.
Abstract Iron is an essential nutrient that may exert toxic effects when it accumulates in tissues. Little is known regarding its effects on gonadal function. Both Fe2+ and Fe3+ could be released from iron deposition. We employed mouse non-luteinized granulosa cell for in vitro studies and human ovarian tissues for Prussian blue and immunohistochemical staining to identify the iron deposition and effect in vivo. After treatment with FeSO4-7H2O or FeCl3 in granulosa cell cultured with follicle stimulating hormone (FSH) for 48 hours, we found that Fe2+ significantly suppressed FSH-induced granulosa cell proliferatio...
Source: Biology of Reproduction - August 28, 2017 Category: Reproduction Medicine Authors: Chen MJ, Chou CH, Shun CT, Mao TL, Wen WF, Chen CD, Chen SU, Yang YS, Ho HN Tags: Biol Reprod Source Type: research

Human Endometrial Stromal Cell Decidualization Requires Transcriptional Reprogramming by PLZF.
Abstract Infertility and early embryo-miscarriage is linked to inadequate endometrial decidualization. Although transcriptional reprogramming is known to drive decidualization in response to progesterone, the key signaling effectors that directly mediate this hormone response are not fully known. This knowledge-gap is clinically significant because identifying the early signals that directly mediate progesterone-driven decidualization will address some of the current limitations in diagnosing and therapeutically treating patients at most risk for early pregnancy loss. We recently revealed that the promyelocytic le...
Source: Biology of Reproduction - November 25, 2017 Category: Reproduction Medicine Authors: Szwarc MM, Hai L, Gibbons WE, Peavey MC, White LD, Mo Q, Lonard DM, Kommagani R, Lanz RB, DeMayo FJ, Lydon JP Tags: Biol Reprod Source Type: research

Porcine Parvovirus infection impairs progesterone production in Luteal Cells through MAPKs, p53 and mitochondria-mediated Apoptosis.
Abstract Porcine parvovirus (PPV) is a major virus leading to fetal death in swine. However, the effects of PPV infection on sows are poorly understood. The aim of this study was to investigate the effects of PPV on porcine steroidogenic luteal cells (SLCs) survival and functions and underlying mechanisms. In vivo experiment results showed artificial infection of PPV significantly reduced the concentration of serum progesterone and induced histopathological lesions and SLCs apoptosis in porcine corpora luteum. In vitro cultured primary porcine SLCs, PPV could infect and replicate in SLCs and induced SLCs apoptosis...
Source: Biology of Reproduction - January 18, 2018 Category: Reproduction Medicine Authors: Zhang L, Wang Z, Zhang J, Luo X, Du Q, Chang L, Zhao X, Huang Y, Tong D Tags: Biol Reprod Source Type: research

Long noncoding RNAs exchange during zygotic genome activation in goat.
Abstract In mammals, their proper development during the early cleavage stages strongly relies on the gene products newly transcribed by zygotic genome activation (ZGA). Long noncoding RNAs (lncRNAs) have been characterized as key regulators of the ZGA process in mice and human. However, the ZGA stage has not yet been identified and epigenetic regulations of the ZGA process remain largely unknown in goats. Here, we show that ZGA occurred at the 8-cell stage in goats. During ZGA, tri-methylation of H3K9 was dynamically changed but maintained strong staining in development arrested embryos. Using single-cell RNA seq...
Source: Biology of Reproduction - May 15, 2018 Category: Reproduction Medicine Authors: Deng M, Wan Y, Liu Z, Ren C, Zhang G, Pang J, Zhang Y, Wang F Tags: Biol Reprod Source Type: research

Rosiglitazone augments antioxidant response in the human trophoblast and prevents apoptosis.
In this study, we first examined whether rosiglitazone, an agonist of the peroxisome proliferator-activated receptor-γ (PPARγ), protects the human trophoblast from oxidative injury by regulating key antioxidant proteins, catalase (CAT) and the superoxide dismutases (SOD1 and SOD2). In first trimester placental explants, localization of CAT was limited to cytotrophoblasts, whereas SOD1 was expressed in both the cyto- and syncytiotrophoblasts. In first trimester placental explants, hypoxia decreased the expression of both SOD1 and SOD2, and increased apoptosis. Treatment with rosiglitazone dose-dependently upregulated anti...
Source: Biology of Reproduction - August 18, 2018 Category: Reproduction Medicine Authors: Kohan-Ghadr HR, Kilburn BA, Kadam L, Johnson E, Kolb BL, Rodriguez-Kovacs J, Hertz M, Armant DR, Drewlo S Tags: Biol Reprod Source Type: research

HMGA2 is regulated by LIN28 and BRCA1 in human placental cells †.
HMGA2 is regulated by LIN28 and BRCA1 in human placental cells†. Biol Reprod. 2018 Aug 18;: Authors: West RC, McWhorter ES, Ali A, Goetzman LN, Russ JE, Anthony RV, Bouma GJ, Winger QA Abstract The chromatin associated transcription factor HMGA2 is a downstream target of let-7 miRNAs and binds to chromatin to regulate gene expression. Inhibition of let-7 miRNAs by RNA binding proteins LIN28A and LIN28B is necessary during early embryogenesis to ensure stable expression of HMGA2. In addition to LIN28, HMGA2 is regulated by a BRCA1/ZNF350/CtIP repressor complex. In normal tissues, the BRCA1/ZNF350/CtI...
Source: Biology of Reproduction - August 18, 2018 Category: Reproduction Medicine Authors: West RC, McWhorter ES, Ali A, Goetzman LN, Russ JE, Anthony RV, Bouma GJ, Winger QA Tags: Biol Reprod Source Type: research