MicroRNA-183~96~182 Cluster Regulate Bovine Granulosa Cell Proliferation and Cell Cycle Transition by Coordinately Targeting FOXO1.

MicroRNA-183~96~182 Cluster Regulate Bovine Granulosa Cell Proliferation and Cell Cycle Transition by Coordinately Targeting FOXO1. Biol Reprod. 2016 Apr 27; Authors: Gebremedhn S, Salilew-Wondim D, Hoelker M, Rings F, Neuhoff C, Tholen E, Schellander K, Tesfaye D Abstract Large-scale expression profiling of microRNAs (miRNAs) in bovine granulosa cells from dominant and subordinate follicles on Day 19 of the estrous cycle revealed enriched microRNA-183~96~182 (miR-183~96~182) cluster miRNAs in preovulatory dominant follicles that coordinately regulate the forkhead box protein O1 (FOXO1) gene. However, little is known about the role of this cluster in bovine granulosa cell function. We employed an in vitro granulosa cell culture model to investigate this role. Granulosa cells aspirated from small growing follicles (3-5 mm in diameter) were cultured in DMEM/F-12 medium supplemented with Fetal Bovine Serum (FBS) and transfected with Locked Nucleic Acid (LNA)-based miRNA mimics, inhibitors, and the corresponding negative controls. The overexpression of the miRNA cluster resulted in the suppression of FOXO1 mRNA and protein, while inhibition of the cluster increased the expression of FOXO1 mRNA. Overexpression also increased the relative rate of cell proliferation, while inhibition slowed it down. Similarly, the proportion of cells under G0/G1 arrest declined, while the ratio of cells in the S phase increased in response to miR-183~96~182...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research