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Dengue virus population genetics in Yogyakarta, Indonesia prior to city-wide Wolbachia deployment
Infect Genet Evol. 2022 May 26;102:105308. doi: 10.1016/j.meegid.2022.105308. Online ahead of print.ABSTRACTDengue has been endemic in Yogyakarta, Indonesia for decades. Here, we report the dengue epidemiology, entomology, and virology in Yogyakarta in 2016-2017, prior to the commencement of the Applying Wolbachia to Eliminate Dengue (AWED) randomized trial. Dengue epidemiological data were compiled and blood samples from dengue-suspected patients were tested for dengue virus (DENV). Ae. aegypti mosquito samples were caught from the field using BG-Sentinel traps and tested for the presence of DENV infection. Sequencing of ...
Source: Infection, Genetics and Evolution - June 1, 2022 Category: Genetics & Stem Cells Authors: Eggi Arguni Citra Indriani Ayu Rahayu Endah Supriyati Benediktus Yohan Rahma F Hayati Satrio Wardana Warsito Tantowijoyo Muhammad Ridwan Anshari Endang Rahayu None Rubangi Riris Andono Ahmad Adi Utarini Cameron P Simmons R Tedjo Sasmono Source Type: research

Molecular phylogeny of Monomorium pharaonis (Hymenoptera: Formicidae) based on rRNA sequences of mitochondrial gene.
In this study, using mitochondrial DNA knowledge, the molecular analysis was performed to classify the recovered species of hymenoptera collected from rabbit carcass, quickly and accurately. A molecular identification method with a 251-bp fragment of the 16S ribosomal gene RNA (16S rRNA) from a single ant species was evaluated. The maximum likelihood method analysis has recovered a generally well-supported phylogeny, with most taxa and species groups currently being recognized as monophyletic. The aculeate consistsof some Hymenoptera's best known. Their sister group has traditionally been considered in Ichneumonoidea. In a...
Source: Journal of Genetics - June 4, 2020 Category: Genetics & Stem Cells Authors: Alajmi R, Haddadi R, Abdel-Gaber R, Alkuriji M Tags: J Genet Source Type: research

Expanding Research Capacity in Sub-Saharan Africa Through Informatics, Bioinformatics, and Data Science Training Programs in Mali
Conclusion Bioinformatics and data science training programs in developing countries necessitate incremental and collaborative strategies for their feasible and sustainable development. The progress described here covered decades of collaborative efforts centered on training and research on computationally intensive topics. These efforts laid the groundwork and platforms conducive for hosting a bioinformatics and data science training program in Mali. Training programs are perhaps best facilitated through Africa’s university systems as they are perhaps best positioned to maintain core resources during lapses in sho...
Source: Frontiers in Genetics - April 11, 2019 Category: Genetics & Stem Cells Source Type: research

Genes, Vol. 9, Pages 153: Dating Pupae of the Blow Fly Calliphora vicina Robineau –Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers
Genes, Vol. 9, Pages 153: Dating Pupae of the Blow Fly Calliphora vicina Robineau–Desvoidy 1830 (Diptera: Calliphoridae) for Post Mortem Interval—Estimation: Validation of Molecular Age Markers Genes doi: 10.3390/genes9030153 Authors: Barbara Zajac Jens Amendt Marcel Verhoff Richard Zehner Determining the age of juvenile blow flies is one of the key tasks of forensic entomology when providing evidence for the minimum post mortem interval. While the age determination of blow fly larvae is well established using morphological parameters, the current study focuses on molecular methods for estimating the age ...
Source: Genes - March 9, 2018 Category: Genetics & Stem Cells Authors: Barbara Zajac Jens Amendt Marcel Verhoff Richard Zehner Tags: Article Source Type: research

Outline-based Morphometrics, an overlooked method in arthropod studies?
We present a few examples highlighting its interest for separating close or cryptic species, or characterizing conspecific geographic populations, in a series of different vector organisms. In this simple application, i.e. the recognition of close or cryptic forms, the outline approach provided similar scores as those obtained by the landmark-based approach. PMID: 25111609 [PubMed - as supplied by publisher]
Source: Infection, Genetics and Evolution - August 8, 2014 Category: Genetics & Stem Cells Authors: Dujardin JP, Kaba D, Solano P, Dupraz M, McCoy KD, Jaramillo-O N Tags: Infect Genet Evol Source Type: research

Identification of cryptic Anopheles mosquito species by molecular protein profiling.
Abstract Vector control is the mainstay of malaria control programmes. Successful vector control profoundly relies on accurate information on the target mosquito populations in order to choose the most appropriate intervention for a given mosquito species and to monitor its impact. An impediment to identify mosquito species is the existence of morphologically identical sibling species that play different roles in the transmission of pathogens and parasites. Currently PCR diagnostics are used to distinguish between sibling species. PCR based methods are, however, expensive, time-consuming and their development requ...
Source: Genomics Proteomics ... - May 22, 2013 Category: Genetics & Stem Cells Authors: Müller P, Pflüger V, Wittwer M, Ziegler D, Chandre F, Simard F, Lengeler C Tags: PLoS One Source Type: research