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Prolylcarboxypeptidase promotes angiogenesis and vascular repair
Prolylcarboxypeptidase (PRCP) is associated with leanness, hypertension, and thrombosis. PRCP-depleted mice have injured vessels with reduced Kruppel-like factor (KLF)2, KLF4, endothelial nitric oxide synthase (eNOS), and thrombomodulin. Does PRCP influence vessel growth, angiogenesis, and injury repair? PRCP depletion reduced endothelial cell growth, whereas transfection of hPRCP cDNA enhanced cell proliferation. Transfection of hPRCP cDNA, or an active site mutant (hPRCPmut) rescued reduced cell growth after PRCP siRNA knockdown. PRCP-depleted cells migrated less on scratch assay and murine PRCPgt/gt aortic segments had ...
Source: Blood - August 22, 2013 Category: Hematology Authors: Adams, G. N., Stavrou, E. X., Fang, C., Merkulova, A., Alaiti, M. A., Nakajima, K., Morooka, T., Merkulov, S., LaRusch, G. A., Simon, D. I., Jain, M. K., Schmaier, A. H. Tags: Vascular Biology Source Type: research

Microparticle-associated tissue factor is recycled by endothelial cells resulting in enhanced surface tissue factor activity.
In this study the uptake of tissue factor (TF)-positive microparticles by endothelial cells and the recycling of the TF component were examined. Human dermal blood endothelial cells (HDBEC) were incubated with microparticles derived from cancer cell lines for up to 6 hours. Measurement of HDBEC cell surface TF antigen revealed two distinct peaks at 30 and 180-240 minutes post-incubation with TF-positive, but not TF-deficient microparticles. However, only the second peak was concurrent with high TF activity as determined by a chromogenic thrombin-generation assay. Annexin V-labelling of HDBEC showed phosphatidylserine expos...
Source: Thrombosis and Haemostasis - August 15, 2013 Category: Hematology Authors: Collier ME, Mah PM, Xiao Y, Maraveyas A, Ettelaie C Tags: Thromb Haemost Source Type: research

Acute and severe coagulopathy in adult mice following silencing of hepatic antithrombin and protein C production
Mice deficient in the anticoagulants antithrombin (Serpinc1) or protein C (Proc) display premature death due to thrombosis-related coagulopathy, thereby precluding their use in gene function studies and thrombosis models. We used RNA interference to silence Serpinc1 and/or Proc in normal adult mice. The severe coagulopathy that followed combined "knockdown" of these genes is reported. Two days after siRNA injection, thrombi (occlusive) were observed in vessels (large and medium-sized) in multiple tissues, and hemorrhages were prominent in the ocular, mandibular, and maxillary areas. Tissue fibrin deposition and reduction o...
Source: Blood - May 23, 2013 Category: Hematology Authors: Safdar, H., Cheung, K. L., Salvatori, D., Versteeg, H. H., Laghmani, E. H., Wagenaar, G. T. M., Reitsma, P. H., van Vlijmen, B. J. M. Tags: Thrombosis and Hemostasis, Brief Reports Source Type: research

siRNA down-regulation of FGA mRNA in HepG2 cells demonstrated that heterozygous abnormality of the Aα-chain gene does not affect the plasma fibrinogen level
Conclusions: Our results suggest that FGG mRNA levels limit fibrinogen expression in normal liver and HepG2 cells and that 50% reduction of FGA mRNA levels would not limit fibrinogen expression in normal liver and HepG2 cells.
Source: Thrombosis Research - February 15, 2013 Category: Hematology Authors: Yuka Takezawa, Kazuyuki Matsuda, Fumiko Terasawa, Mitsutoshi Sugano, Takayuki Honda, Nobuo Okumura Tags: Coagulation and Fibrinolysis Source Type: research

RAD001-mediated STAT3 upregulation and megakaryocytic differentiation.
Conclusion, the present study demonstrated that RAD001 might have the capacity to induce MK differentiation through the up-regulation of STAT3 signalling. PMID: 23329056 [PubMed - as supplied by publisher]
Source: Thrombosis and Haemostasis - January 17, 2013 Category: Hematology Authors: Su YC, Li SC, Peng HY, Ho YH, Chen LJ, Liao HF Tags: Thromb Haemost Source Type: research

Distinct roles for the α , β and γ1 isoforms of protein phosphatase 1 in the outside-in αIIbβ3 integrin signalling-dependent functions.
Abstract Although protein kinases and phosphatases participate in integrin αIIbβ3 signalling, whether integrin functions are regulated by the catalytic subunit of protein phosphatase 1(PP1c)isoforms are unclear. We show that siRNA mediated knockdown of all PP1c isoforms(α, β and γ1)in 293 αIIbβ3 cells decreased adhesion to immobilised fibrinogen and fibrin clot retraction. Selective knockdown of only PP1cγ1 did not alter adhesion or clot retraction, while depletion of PP1cβ decreased both functions. Unexpectedly, knockdown of PP1cα enhanced αIIbβ3 adhesion to fibrinogen and clot retraction. Protein int...
Source: Thrombosis and Haemostasis - January 8, 2013 Category: Hematology Authors: Alrehani N, Pradhan S, Khatlani T, Kailasam L, Vijayan KV Tags: Thromb Haemost Source Type: research

Integrin-substrate interactions underlying shear-induced inhibition of the inflammatory response of endothelial cells.
Abstract Conditioning of endothelial cells by shear stress suppresses their response to inflammatory cytokines. We questioned whether signalling through different integrin-matrix interactions, previously associated with the pathogenic effects of disturbed flow, supported the anti-inflammatory action of steady shear. Primary human endothelial cells were cultured on different substrates and exposed to shear stress (2.0Pa) for varying periods before stimulation with tumour necrosis factor-α (TNF). Shear-conditioning inhibited cytokine-induced recruitment of flowing neutrophils. However, the effect was similar for cu...
Source: Thrombosis and Haemostasis - December 13, 2012 Category: Hematology Authors: Luu NT, Glen KE, Egginton S, Rainger GE, Nash GB Tags: Thromb Haemost Source Type: research

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