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Laminins and Nidogens in the Pericellular Matrix of Chondrocytes
The aim of this study was to investigate the role of laminins and nidogen-2 in osteoarthritis (OA) and their potential to support chondrogenic differentiation. We applied immunohistochemistry, electron microscopy, siRNA, quantitative RT-PCR, Western blot, and proteome analysis for the investigation of cartilage tissue and isolated chondrocytes in three-dimensional culture obtained from patients with late-stage knee OA and nidogen-2 knockout mice. We demonstrate that subunits of laminins appear in OA cartilage and that nidogen-2–null mice exhibit typical osteoarthritic features.
Source: American Journal of Pathology - December 9, 2015 Category: Pathology Authors: Boris Schminke, Jenny Frese, Christa Bode, Mary B. Goldring, Nicolai Miosge Tags: Regular Article Source Type: research

Laminins and Nidogens in the Pericellular Matrix of Chondrocytes: Their Role in Osteoarthritis and Chondrogenic Differentiation.
This study reveals that the influence of the pericellular matrix on CPCs is important for the expression of the major regulator transcription factors, SOX9 and RUNX2. Our novel findings that laminins and nidogen-2 drive CPCs toward chondrogenesis may help in the elucidation of new treatment strategies for cartilage tissue regeneration. PMID: 26683663 [PubMed - as supplied by publisher]
Source: The American Journal of Pathology - December 10, 2015 Category: Pathology Authors: Schminke B, Frese J, Bode C, Goldring MB, Miosge N Tags: Am J Pathol Source Type: research

Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1 β-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte.
Histone Deacetylase Inhibitor Vorinostat (SAHA) Suppresses IL-1β-Induced Matrix Metallopeptidase-13 Expression by Inhibiting IL-6 in Osteoarthritis Chondrocyte. Am J Pathol. 2016 Aug 20; Authors: Makki MS, Haqqi TM Abstract Osteoarthritis (OA) is the most common whole-joint disease and is characterized by progressive loss of the cartilage matrix. Matrix metallopeptidase-13 (MMP-13) is a highly active and an abundantly expressed protease in OA cartilage and chondrocytes and degrades type II collagen and proteoglycans. We investigated the mechanism of MMP-13 suppression by histone deacetylase inhibitor...
Source: The American Journal of Pathology - August 19, 2016 Category: Pathology Authors: Makki MS, Haqqi TM Tags: Am J Pathol Source Type: research

Heparan Sulfate Proteoglycan Synthesis is Dysregulated in Human Osteoarthritic Cartilage.
Abstract Osteoarthritis (OA) is a common degenerative joint disease, characterized by cartilage loss and subchondral bone remodelling in response to abnormal mechanical load. Heparan sulfate (HS) proteoglycans bind to many proteins that regulate cartilage homeostasis, including growth factors, morphogens, proteases, and their inhibitors, and modulate their localization, retention, and biological activity. Changes in HS expression and structure may thus have important consequences for joint health. We analyzed normal and osteoarthritic human knee cartilage, and found HS biosynthesis was markedly disrupted in OA, wi...
Source: The American Journal of Pathology - December 13, 2018 Category: Pathology Authors: Chanalaris A, Clarke H, Guimond SE, Vincent TL, Turnbull JE, Troeberg L Tags: Am J Pathol Source Type: research

SOX11 promotes osteoarthritis through induction of TNF-α
ConclusionInhibition of SOX11 could improve IL-1β-induced OA like phenomenon in CHON-001 cells, which suggesting SOX11 played an important role during the pathogenesis of OA. Thus, we hypothesized that SOX11 could be a potential target for the treatment of patients with OA.
Source: Pathology Research and Practice - May 7, 2019 Category: Pathology Source Type: research

SOX11 promotes osteoarthritis through induction of TNF- α.
CONCLUSION: Inhibition of SOX11 could improve IL-1β-induced OA like phenomenon in CHON-001 cells, which suggesting SOX11 played an important role during the pathogenesis of OA. Thus, we hypothesized that SOX11 could be a potential target for the treatment of patients with OA. PMID: 31078342 [PubMed - as supplied by publisher]
Source: Pathology, Research and Practice - May 5, 2019 Category: Pathology Authors: Xu S, Yu J, Wang Z, Ni C, Xia L, Tang T Tags: Pathol Res Pract Source Type: research

Implication of cell migration inducing hyaluronidase 1 (CEMIP) in hyaluronan degradation by synovial fibroblasts in patients with knee osteoarthritis.
This study examined their expression in synovial tissue and the relationship with molecular weight of HA in SF in knee osteoarthritis patients. Quantification of mRNA demonstrated that HYBID expression is significantly (5.4-fold) higher in osteoarthritic synovium than in normal control synovium, whereas TMEM2 expression level is similar between the two groups. By immunohistochemistry, HYBID was localized mainly to CD68-negative and fibroblast-specific protein 1-positive synovial lining cells and sub-lining fibroblasts in osteoarthritic synovium. The mRNA expression levels of HYBID, but not TMEM2, in osteoarthritic synovium...
Source: The American Journal of Pathology - February 17, 2020 Category: Pathology Authors: Shiozawa J, de Vega S, Cilek MZ, Yoshinaga C, Nakamura T, Kasamatsu S, Yoshida H, Kaneko H, Ishijima M, Kaneko K, Okada Y Tags: Am J Pathol Source Type: research

GAB2 inhibits chondrocyte apoptosis through PI3K-AKT signaling in osteoarthritis.
Abstract Cartilage degeneration is considered the main pathologic feature of osteoarthritis (OA). Cumulative evidence indicates that chondrocyte apoptosis is associated with cartilage degradation. However, the underlying molecular mechanism of chondrocyte apoptosis remains unclear. Growth factor receptor-bound protein 2 (GAB2), an adaptor protein, belongs to the Gab family and is involved in various biologic processes. Here, we explored the role of GAB2 in the pathogenesis of osteoarthritis (OA). GAB2 expression was markedly increased in OA articular cartilage. GAB2 expression was also increased in an in vitro mod...
Source: International Journal of Clinical and Experimental Pathology - April 10, 2020 Category: Pathology Authors: Cheng Z, Sun W, Ni X, Xu H, Wang Y Tags: Int J Clin Exp Pathol Source Type: research