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Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells.
In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OC...
Source: Oncology Research - March 6, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer By Sponging MiR-135a.
This study is aimed to investigate the role of urothelial carcinoma associated 1(UCA1) in the progression of PC. Our results revealed that long non-coding RNAs (lncRNAs)-UCA1 was overexpressed in PC tissues compared with adjacent histologically normal tissues. Down-regulated level of UCA1 was also detected in five human pancreatic cancer cell lines (SW1990，BxPC-3, MiaPaCa-2, PANC-1, CAPAN-1) compared with normal pancreatic duct epithelial HPDE cells. The proliferation of PC cells was inhibited after UCA1 was suppressed by a lentiviral vector. Cell apoptosis rate was largely promoted by down-regulating UCA1. Further resea...
Source: Oncology Research - March 23, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Leptin promotes metastasis by inducing an epithelial-mesenchymal transition in a549 lung cancer cells.
Abstract Leptin, an adipocyte-derived cytokine associated with obesity, has been reported to participate in carcinogenesis. Epithelial-mesenchymal transition (EMT) is also considered as a key event in tumor metastasis. The aim of this study is to investigate the mechanism of leptin in the promotion of EMT leading to metastasis in A549 lung cancer cells. We investigated the effect of leptin on migration of A549 cells using wound healing and transwell assays. The incidence of EMT in A549 cells was examined by real-time PCR and immunofluorescence staining. The expression of TGF-β in A549 cells was detected by real-t...
Source: Oncology Research - February 17, 2014 Category: Cancer & Oncology Authors: Feng H, Liu Q, Zhang N, Zheng L, Sang M, Feng J, Zhang J, Wu X, Shan B Tags: Oncol Res Source Type: research

PAR2 inhibition enhanced the sensitivity of colorectal cancer cells to 5-FU and reduced EMT signaling.
In this study, we found that protease-activated receptor-2 (PAR2) induction in 5-Fu therapy is correlated with TGF-β-mediated EMT and apoptosis resistance. PAR2 and TGF-β were both activated in response to 5-Fu treatment in vivo and in vitro, and whereas TGF-β inhibition sensitized CRC cells to 5-Fu and suppressed cell migration, PAR2 activation eliminated the effect TGF-β inhibition. Conversely, siRNA-mediated PAR2 depletion or PAR2 inhibition with a specific inhibitor produced a similar phenotype as TGF-β signal inhibition: 5-Fu sensitization and cell-migration suppression. Moreover, the results of xenograft experim...
Source: Oncology Research - March 9, 2019 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of PFTK1 Expression by RNAi Inhibits the Proliferation and Invasion of Human Non-Small Lung Adenocarcinoma Cells.
In this study, we aimed to explore the expression and function of PFTK1 in NSCLC cells. Our results showed that PFTK1 was significantly upregulated in human NSCLC cell lines. Silencing the expression of PFTK1 inhibited the proliferation of NSCLC cells. In addition, silencing the expression of PFTK1 endowed NSCLC cells with decreased migration and invasion abilities, as well as epithelial-mesenchymal transition (EMT) progress in A549 cells. A mechanistic study showed that knockdown of PFTK1 inhibited the expression of β-catenin, cyclin D1, and c-Myc in A549 cells. In summary, we report that small interfering RNA (siRNA)-PF...
Source: Oncology Research - July 29, 2016 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Gastrin enhances autophagy and promotes gastric carcinoma proliferation via inducing AMPK α.
In conclusion, these findings indicate that gastrin plays a tumorigenic role by promoting autophagy in gastric cancer, and may provide a novel therapeutic target for gastric cancer treatment. PMID: 28059052 [PubMed - as supplied by publisher]
Source: Oncology Research - January 9, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Knockdown of DDX5 inhibits the proliferation and tumorigenesis in esophageal cancer.
In conclusion, our findings provide the first evidence that siRNA-DDX5 inhibited the proliferation and invasion of EC cells through suppressing the Wnt/β-catenin signaling pathway. Therefore, DDX5 may be a novel potential therapeutic target for the prevention and treatment of EC. PMID: 28244855 [PubMed - as supplied by publisher]
Source: Oncology Research - March 2, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line.
This study aimed to investigate the pivotal role of cystatin B (CSTB) in the development of gastric cancer and to explore its possible regulatory mechanism. Human gastric cancer SGC-7901 cells as a model in vitro were transfected with plasmid PCDNA3.1-CSTB and siRNA-CSTB using Lipofectamine 2000. Quantitative real-time PCR (qRT-PCR) and Western blotting were performed to determine the relative expression of CSTB and PI3K/Akt/mTOR pathway-related protein. Moreover, MTT assay, Transwell assay, and flow cytometry were used to assess cell proliferation, migration, and apoptosis, respectively. The results showed that CSTB was s...
Source: Oncology Research - March 12, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line.
In conclusion, miR-183 contributed to cell growth and proliferation, and suppressed cell apoptosis in SCC25 cells. Therefore, miR-183 might serve as a therapeutic target in tongue squamous cell carcinoma (TSCC). PMID: 28281960 [PubMed - in process]
Source: Oncology Research - March 12, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

Activation of Vimentin is Critical to Promote a Metastatic Potential of Cholangiocarcinoma Cells.
Authors: Saentaweesuk W, Araki N, Vaeteewoottacharn K, Silsirivanit A, Seubwai W, Talabnin C, Muisuk K, Sripa B, Wongkham S, Okada S, Wongkham C Abstract Cholangiocarcinoma (CCA) is a highly metastatic tumor, of which the majority of the patients have short survival due tono effective treatment. Hence, a better understanding regarding CCA metastasis may provide an opportunity to improve the strategies for treatment. A comparison study between the highly metastatic cells and their parental cells is an approach to uncover the molecular mechanisms underlying the metastatic process. In the present study, a lung metasta...
Source: Oncology Research - August 3, 2017 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

The downregulation of miR-200c promotes lactate dehydrogenase A expression and non-small cell lung cancer progression.
This study was aimed to investigate the function and mechanism of microRNA-200c (miR-200c) in the progression of non-small cell lung cancer (NSCLC). A total of 76 patients diagnosed as NSCLC were enrolled in this study. Then, the expression level of miR-200c in NSCLC tissues and cell lines was investigated using the quantitative realtime polymerase chain reaction (RT-qPCR) method. We found the expression of miR200c was significantly reduced in NSCLC tissues and cell lines compared with in normal lung tissues and human bronchial epithelial cell line. Force the expression of miR-200c using miR-200c mimic significantly suppre...
Source: Oncology Research - January 14, 2018 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

EIF5A2 is highly expressed in anaplastic thyroid carcinoma and is associated with tumor growth by modulating TGF- β signals.
EIF5A2 is highly expressed in anaplastic thyroid carcinoma and is associated with tumor growth by modulating TGF-β signals. Oncol Res. 2020 Mar 05;: Authors: Huang Y, Zhu Q, Lu L, Sun S, Hao F, Zhang J, Liu Z, Miao Y, Jiao X, Chen D Abstract Anaplastic thyroid carcinoma (ATC) are resistant to standard therapies and has no effective treatment. Eukaryotic translation initiation factor 5A2 (EIF5A2) has shown to be upregulated inmany malignant tumors and proposed to be a critical gene involved in tumor metastasis. In thisstudy, we aim to investigate the expression status of EIF5A2 in human ATC tissues, an...
Source: Oncology Research - March 8, 2020 Category: Cancer & Oncology Tags: Oncol Res Source Type: research