Sugar Utilization-Associated Food-Grade Selection Markers in Lactic Acid Bacteria and Yeast
Pol J Microbiol. 2024 Mar 4;73(1):3-10. doi: 10.33073/pjm-2024-011. eCollection 2024 Mar 1.ABSTRACTThis comprehensive review explores the development of food-grade selection markers in lactic acid bacteria and yeast; some of their strains are precisely defined as safe microorganisms and are crucial in the food industry. Lactic acid bacteria, known for their ability to ferment carbohydrates into lactic acid, provide essential nutrients and contribute to immune responses. With its strong fermentation capabilities and rich nutritional profile, yeast finds use in various food products. Genetic engineering in these microorganis...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Zhiwen Liang Ke Zheng Guifeng Xie Xiongsheng Luo Huangjin Li Source Type: research

Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a
Pol J Microbiol. 2024 Mar 4;73(1):89-97. doi: 10.33073/pjm-2024-009. eCollection 2024 Mar 1.ABSTRACTNorovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on R...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Xinyi Hu Pei He Tong Jiang Jilu Shen Source Type: research

Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of < em > Staphylococcus aureus < /em > in Lithuania
This study aimed to determine resistance to antimicrobials of Staphylococcus aureus strains isolated from clinical specimens in Lithuanian hospitals and to identify the genes conferring resistance and virulence. The study was carried out from June 2019 to September 2021. S. aureus strains were isolated from skin, soft tissues, blood, lower respiratory tract, urine and other specimens. Antibiotic susceptibility testing was performed using the disc diffusion method according to EUCAST guidelines. All isolates were analyzed for detection of the ermA, ermC, mecA, mecC, tetK, tetM, and lukF-PV genes by multiplex real-time PCR. ...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Agn ė Kirkliauskienė Jonas Kri ščiūnas Jolanta Miciulevi čienė Daiva Radzi šauskienė Tomas Ka čergius Maksim Bratchikov Lina Kaplerien ė Source Type: research

Association between Clinical Characteristics and Microbiota in Bronchiectasis Patients Based on Metagenomic Next-Generation Sequencing Technology
This study aimed to investigate the disparities between metagenomic next-generation sequencing (mNGS) and conventional culture results in patients with bronchiectasis. Additionally, we sought to investigate the correlation between the clinical characteristics of patients and their microbiome profiles. The overarching goal was to enhance the effective management and treatment of bronchiectasis patients, providing a theoretical foundation for healthcare professionals. A retrospective survey was conducted on 67 bronchiectasis patients admitted to The First Hospital of Jiaxing from October 2019 to March 2023. Clinical baseline...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Dongfeng Shen Xiaodong Lv Hui Zhang Chunyuan Fei Jing Feng Jiaqi Zhou Linfeng Cao Ying Ying Na Li Xiaolong Ma Source Type: research

Biocontrol and Growth Promotion Potential of < em > Bacillus subtilis < /em > CTXW 7-6-2 against < em > Rhizoctonia solani < /em > that Causes Tobacco Target Spot Disease
In conclusion, B. subtilis CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.PMID:38437465 | PMC:PMC10911660 | DOI:10.33073/pjm-2024-004 (Source: Polish Journal of Microbiology)
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Ning Huang Xin Jin Jin-Tao Wen Yi-Fei Zhang Xu Yang Guang-Yu Wei Yi-Kun Wang Min Qin Source Type: research