Association between Clinical Characteristics and Microbiota in Bronchiectasis Patients Based on Metagenomic Next-Generation Sequencing Technology
This study aimed to investigate the disparities between metagenomic next-generation sequencing (mNGS) and conventional culture results in patients with bronchiectasis. Additionally, we sought to investigate the correlation between the clinical characteristics of patients and their microbiome profiles. The overarching goal was to enhance the effective management and treatment of bronchiectasis patients, providing a theoretical foundation for healthcare professionals. A retrospective survey was conducted on 67 bronchiectasis patients admitted to The First Hospital of Jiaxing from October 2019 to March 2023. Clinical baseline...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Dongfeng Shen Xiaodong Lv Hui Zhang Chunyuan Fei Jing Feng Jiaqi Zhou Linfeng Cao Ying Ying Na Li Xiaolong Ma Source Type: research

Biocontrol and Growth Promotion Potential of < em > Bacillus subtilis < /em > CTXW 7-6-2 against < em > Rhizoctonia solani < /em > that Causes Tobacco Target Spot Disease
In conclusion, B. subtilis CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.PMID:38437465 | PMC:PMC10911660 | DOI:10.33073/pjm-2024-004 (Source: Polish Journal of Microbiology)
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Ning Huang Xin Jin Jin-Tao Wen Yi-Fei Zhang Xu Yang Guang-Yu Wei Yi-Kun Wang Min Qin Source Type: research

Molecular and metabolic characterization of petroleum hydrocarbons degrading < em > Bacillus cereus < /em >
Pol J Microbiol. 2024 Mar 4;73(1):107-120. doi: 10.33073/pjm-2024-012. eCollection 2024 Mar 1.ABSTRACTHydrocarbon constituents of petroleum are persistent, bioaccumulated, and bio-magnified in living tissues, transported to longer distances, and exert hazardous effects on human health and the ecosystem. Bioaugmentation with microorganisms like bacteria is an emerging approach that can mitigate the toxins from environmental sources. The present study was initiated to target the petroleum-contaminated soil of gasoline stations situated in Lahore. Petroleum degrading bacteria were isolated by serial dilution method followed b...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Nadia Hussain Fatima Muccee Muhammad Hammad Farhan Mohiuddin Saboor Muarij Bunny Aansa Shahab Source Type: research

Molecular Detection of a Pathogenic < em > Entamoeba < /em > among Symptomatic Children in Eastern Kurdistan of Iraq
Pol J Microbiol. 2024 Mar 4;73(1):99-105. doi: 10.33073/pjm-2024-010. eCollection 2024 Mar 1.ABSTRACTEntamoeba histolytica infects the large intestine of humans, causing a spectrum of clinical appearances ranging from asymptomatic colonization to severe intestinal and extra-intestinal disease. The parasite is identical microscopically to commensal nonpathogenic amoeba. To detect the pathogenic Entamoeba and estimate the precise prevalence of the parasite among the symptomatic pediatric population using molecular techniques. 323 fecal samples were collected from symptomatic children admitted to Sulaimani Pediatric Teaching ...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Sham Jamil Abdullah Shahnaz Abdulkader Ali Source Type: research

A Mendelian Randomization Study: Roles of Gut Microbiota in Sepsis - Who is the Angle?
This study implied at different taxa levels Actinobacteria and Bifidobacteriaceae, Tyzzerella genus, and Gastranaerophilales order have a causal relationship with sepsis, indicating that they are protective factors for the incidence of sepsis.PMID:38437468 | PMC:PMC10911657 | DOI:10.33073/pjm-2024-006 (Source: Polish Journal of Microbiology)
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Yeping Bian Jian Xu Xiaojing Deng Suming Zhou Source Type: research

Increased Proteolytic Activity of < em > Serratia marcescens < /em > Clinical Isolate HU1848 Is Associated with Higher < em > eepR < /em > Expression
Pol J Microbiol. 2024 Mar 4;73(1):11-20. doi: 10.33073/pjm-2024-002. eCollection 2024 Mar 1.ABSTRACTSerratia marcescens is a global opportunistic pathogen. In vitro cytotoxicity of this bacterium is mainly related to metalloprotease serralysin (PrtS) activity. Proteolytic capability varies among the different isolates. Here, we characterized protease production and transcriptional regulators at 37°C of two S. marcescens isolates from bronchial expectorations, HU1848 and SmUNAM836. As a reference strain the insect pathogen S. marcescens Db10 was included. Zymography of supernatant cultures revealed a single (SmUNAM836) or ...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Karla L De Anda-Mora Faviola Tavares-Carre ón Carlos Alvarez Samantha Barahona Miguel A Becerril-Garc ía Rogelio J Trevi ño-Rangel Rodolfo Garc ía-Contreras Angel Andrade Source Type: research

Establishment of RPA-Cas12a-Based Fluorescence Assay for Rapid Detection of Feline Parvovirus
In this study, we incorporated recombinase polymerase amplification (RPA) and Cas12a-mediated detection and developed an RPA-Cas12a-based real-time or end-point fluorescence detection method to identify the NS1 gene of FPV. The total time of RPA-Cas12a-based fluorescence assay is approximately 25 min. The assay presented a limit of detection (LOD) of 1 copies/μl (25 copies/per reaction), with no cross-reactivity with several feline pathogens. The clinical performance of the assay was examined using total genomic DNA purified from 60 clinical specimens and then compared to results obtained with qPCR detection of FPV with 9...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Ting Wang Hao Zeng Qiming Liu Weidong Qian Yongdong Li Jian Liu Rong Xu Source Type: research

Isolation, Identification, Antimicrobial Resistance, Genotyping, and Whole-Genome Sequencing Analysis of < em > Salmonella < /em > Enteritidis Isolated from a Food-Poisoning Incident
Pol J Microbiol. 2024 Mar 4;73(1):69-89. doi: 10.33073/pjm-2024-008. eCollection 2024 Mar 1.ABSTRACTSalmonella enterica is a common pathogen in humans and animals that causes food poisoning and infection, threatening public health safety. We aimed to investigate the genome structure, drug resistance, virulence characteristics, and genetic relationship of a Salmonella strain isolated from patients with food poisoning. The pathogen strain 21A was collected from the feces of patients with food poisoning, and its minimum inhibitory concentration against commonly used antibiotics was determined using the strip test and Kirby-Ba...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Zhuru Hou Benjin Xu Ling Liu Rongrong Yan Jinjing Zhang Source Type: research

Sugar Utilization-Associated Food-Grade Selection Markers in Lactic Acid Bacteria and Yeast
Pol J Microbiol. 2024 Mar 4;73(1):3-10. doi: 10.33073/pjm-2024-011. eCollection 2024 Mar 1.ABSTRACTThis comprehensive review explores the development of food-grade selection markers in lactic acid bacteria and yeast; some of their strains are precisely defined as safe microorganisms and are crucial in the food industry. Lactic acid bacteria, known for their ability to ferment carbohydrates into lactic acid, provide essential nutrients and contribute to immune responses. With its strong fermentation capabilities and rich nutritional profile, yeast finds use in various food products. Genetic engineering in these microorganis...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Zhiwen Liang Ke Zheng Guifeng Xie Xiongsheng Luo Huangjin Li Source Type: research

Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a
Pol J Microbiol. 2024 Mar 4;73(1):89-97. doi: 10.33073/pjm-2024-009. eCollection 2024 Mar 1.ABSTRACTNorovirus is highly infectious and rapidly transmissible and represents a major pathogen of sporadic cases and outbreaks of acute gastroenteritis worldwide, causing a substantial disease burden. Recent years have witnessed a dramatic increase in norovirus outbreaks in China, significantly higher than in previous years, among which GII norovirus is the predominant prevalent strain. Therefore, rapid norovirus diagnosis is critical for clinical treatment and transmission control. Hence, we developed a molecular assay based on R...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Xinyi Hu Pei He Tong Jiang Jilu Shen Source Type: research

Antimicrobial Resistance and the Prevalence of the Panton-Valentine Leukocidin Gene among Clinical Isolates of < em > Staphylococcus aureus < /em > in Lithuania
This study aimed to determine resistance to antimicrobials of Staphylococcus aureus strains isolated from clinical specimens in Lithuanian hospitals and to identify the genes conferring resistance and virulence. The study was carried out from June 2019 to September 2021. S. aureus strains were isolated from skin, soft tissues, blood, lower respiratory tract, urine and other specimens. Antibiotic susceptibility testing was performed using the disc diffusion method according to EUCAST guidelines. All isolates were analyzed for detection of the ermA, ermC, mecA, mecC, tetK, tetM, and lukF-PV genes by multiplex real-time PCR. ...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Agn ė Kirkliauskienė Jonas Kri ščiūnas Jolanta Miciulevi čienė Daiva Radzi šauskienė Tomas Ka čergius Maksim Bratchikov Lina Kaplerien ė Source Type: research

Association between Clinical Characteristics and Microbiota in Bronchiectasis Patients Based on Metagenomic Next-Generation Sequencing Technology
This study aimed to investigate the disparities between metagenomic next-generation sequencing (mNGS) and conventional culture results in patients with bronchiectasis. Additionally, we sought to investigate the correlation between the clinical characteristics of patients and their microbiome profiles. The overarching goal was to enhance the effective management and treatment of bronchiectasis patients, providing a theoretical foundation for healthcare professionals. A retrospective survey was conducted on 67 bronchiectasis patients admitted to The First Hospital of Jiaxing from October 2019 to March 2023. Clinical baseline...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Dongfeng Shen Xiaodong Lv Hui Zhang Chunyuan Fei Jing Feng Jiaqi Zhou Linfeng Cao Ying Ying Na Li Xiaolong Ma Source Type: research

Biocontrol and Growth Promotion Potential of < em > Bacillus subtilis < /em > CTXW 7-6-2 against < em > Rhizoctonia solani < /em > that Causes Tobacco Target Spot Disease
In conclusion, B. subtilis CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.PMID:38437465 | PMC:PMC10911660 | DOI:10.33073/pjm-2024-004 (Source: Polish Journal of Microbiology)
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Ning Huang Xin Jin Jin-Tao Wen Yi-Fei Zhang Xu Yang Guang-Yu Wei Yi-Kun Wang Min Qin Source Type: research

Molecular and metabolic characterization of petroleum hydrocarbons degrading < em > Bacillus cereus < /em >
Pol J Microbiol. 2024 Mar 4;73(1):107-120. doi: 10.33073/pjm-2024-012. eCollection 2024 Mar 1.ABSTRACTHydrocarbon constituents of petroleum are persistent, bioaccumulated, and bio-magnified in living tissues, transported to longer distances, and exert hazardous effects on human health and the ecosystem. Bioaugmentation with microorganisms like bacteria is an emerging approach that can mitigate the toxins from environmental sources. The present study was initiated to target the petroleum-contaminated soil of gasoline stations situated in Lahore. Petroleum degrading bacteria were isolated by serial dilution method followed b...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Nadia Hussain Fatima Muccee Muhammad Hammad Farhan Mohiuddin Saboor Muarij Bunny Aansa Shahab Source Type: research

Molecular Detection of a Pathogenic < em > Entamoeba < /em > among Symptomatic Children in Eastern Kurdistan of Iraq
Pol J Microbiol. 2024 Mar 4;73(1):99-105. doi: 10.33073/pjm-2024-010. eCollection 2024 Mar 1.ABSTRACTEntamoeba histolytica infects the large intestine of humans, causing a spectrum of clinical appearances ranging from asymptomatic colonization to severe intestinal and extra-intestinal disease. The parasite is identical microscopically to commensal nonpathogenic amoeba. To detect the pathogenic Entamoeba and estimate the precise prevalence of the parasite among the symptomatic pediatric population using molecular techniques. 323 fecal samples were collected from symptomatic children admitted to Sulaimani Pediatric Teaching ...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Sham Jamil Abdullah Shahnaz Abdulkader Ali Source Type: research